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2 protocols using polysorbate 80

1

Docetaxel-Based Tumor-Targeted Nanoparticles

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Docetaxel, 3-maleimidopropionic acid, 4-(dimethylamino) pyridine (DMAP), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDCI) were obtained from Sigma–Aldrich (Burlington, MA, USA). Deuterium chloride, dimethyl sulfoxide (DMSO) and dichloromethane (DCM) were purchased from Innochem (Beijing, China). Cy5.5 dye, pyrene, glutaraldehyde, polysorbate 80 and phosphotungstic acid were provided by Macklin (Shanghai, China). APC anti-mouse CD133, FITC anti-mouse CD44, anti-β-tubulin antibody were purchased from Biolegend (San Diego, CA, USA). Hoechst 33,342 and DAPI were purchased from Solarbio (Beijing, China). TRITC-phalloidin was provided by Lablead (Beijing, China). Cell counting kit-8 (CCK-8) was provided by Dojindo Laboratories (Kumamoto, Japan). FITC-gelatin was purchased from AnaSpec (Fremont, CA, USA). RPMI-1640 medium and FBS were acquired from Wisent (Nanjing, China).
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2

Synthesis and Characterization of Multifunctional Nanoparticles

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PVP K90 was purchased from BASF (Germany). Methotrexate (MTX, Mw = 454.44 Da) (purity >98%) was obtained from Heowns OPDE Biochemical Technology Co. Ltd. (Tianjin, China). HA was purchased from Huaxi Biotechnology Co. Ltd. (Jinan, China). Dextran (Dex) was provided by Thermo Fisher Scientific (USA). Bacillus Calmette-Guerin was purchased from the Chengdu Institute of Biological Products Co., Ltd (Sichuan,China). Trypan Blue, stearic acid, and stearyl alcohol were provided by Sinopharm Chemical Reagent Co., Ltd (China). The reagents of acetonitrile (chromatographic grade), anhydrous sodium hydrogen phosphate, liquid paraffin, Span 80, polysorbate 80, glycerinum, and sorbic acid were provided by Macklin (Shanghai, China). Citric acid was from Biotechnology Co. Ltd. (Tianjin, China).
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