Fitc annexin v 7aad apoptosis detection kit

Manufactured by BioLegend

Sourced in United States

The FITC Annexin-V/7AAD Apoptosis Detection Kit is a laboratory product used to detect and quantify apoptosis in cell populations. It utilizes Annexin-V, which binds to phosphatidylserine, and 7-AAD, which stains DNA, to identify cells in early and late stages of apoptosis.

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Lab products found in correlation

Facscanto 2 cytometer, by BD (1 mentions) Fc block, by BioLegend (1 mentions) West pico chemiluminescent kit, by Thermo Fisher Scientific (1 mentions) Pe conjugated mouse anti human cxcr6 antibody, by R&D Systems (1 mentions) Pe conjugated igg2b, by R&D Systems (1 mentions) Apc conjugated igg2a, by R&D Systems (1 mentions) Cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Sybr green supermix, by Bio-Rad (1 mentions)

Close spelling variants of this product

Apoptosis detection kit (47 citations) Annexin V/7AAD (16 citations)

2 protocols using fitc annexin v 7aad apoptosis detection kit

Apoptosis detection in SUM-149 cells

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SUM-149 cells (5 × 10⁵) were seeded and treated either with vehicle (0.1% DMSO) or with 20 μM of EP or with puromycin (200 ng/mL) as positive control for 48 h. After treatment floating cells were collected, and all cells were harvested and counted. Treated cells were analyzed for cell death by using the FITC Annexin-V/7AAD Apoptosis detection kit (product #640922, Biolegend, San Diego, CA, United States) according to the manufacturer’s instructions. Briefly, cells were resuspended in Annexin Binding Buffer, stained with Annexin V-FITC and/or 7-AAD, and incubated for 30 min at 4°C. Data were acquired on a BD FACS Canto II cytometer (BD Biosciences, San José, CA, United States) and analyzed by using FlowJo software (V10, FlowJo, LLC, Ashland, OR, United States).

Martínez-Montemayor M.M., Ling T., Suárez-Arroyo I.J., Ortiz-Soto G., Santiago-Negrón C.L., Lacourt-Ventura M.Y., Valentín-Acevedo A., Lang W.H, & Rivas F. (2019). Identification of Biologically Active Ganoderma lucidum Compounds and Synthesis of Improved Derivatives That Confer Anti-cancer Activities in vitro. Frontiers in Pharmacology, 10, 115.

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CXCR6/CXCL16-Mediated Signaling Pathways

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DTX was purchased from Sigma Aldrich. Human CXCL16 was procured from Peprotech. PE-conjugated mouse anti-human CXCR6 antibody, APC-conjugated rat anti-human CXCL16, respective isotype controls (PE-conjugated IgG2b, APC-conjugated IgG2a), anti-phospho-RelA/NF-κB-p65^(Ser-529), anti-ADAM10, and CXCL16 Quantikine ELISA kit were purchased from R&D Systems. Phospho-NF-κB-p65^(Ser-536), phospho-Erk1/2^{(Thr-202/Tyr-204)}, phospho-GSKβ^(Ser-9), and anti-survivin antibodies were purchased from CST. FITC-Annexin V/7-AAD apoptosis detection kit and Fc-Block were procured from BioLegend. cDNA Synthesis kit, bicinconic acid kit, and West Pico Chemiluminescent kit were obtained from ThermoFisher Scientific. SYBR-Green Supermix and PVDF membranes were purchased from Bio-Rad. Phosphorylation-specific antibody microarray slides were obtained from Fullmoon Biosystems Inc.

Kapur N., Mir H., Sonpavde G., Jain S., Bae S., Lillard JW J.r, & Singh S. (2019). Prostate cancer cells hyper-activate CXCR6 signaling by cleaving CXCL16 to overcome effect of docetaxel. Cancer letters, 454, 1-13.

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