Rat aortic SMC (A7r5 clonal cell line; American Type Culture Collection) were grown in DMEM supplemented with 10% FCS and 1% penicillin/streptomycin, at 37°C in 5% CO2, and used at passages 2 to 12. SMCs were serum-starved before treatment with recombinant rat IFN-γ in the presence or absence of AS-252424 (Sigma-Aldrich) for 48 h.
As 252424
Manufactured by Merck Group
AS-252424 is a laboratory equipment product. It is designed for use in various scientific and research applications. The core function of this product is to facilitate specific experimental procedures or analyses within a controlled laboratory environment.
Automatically generated - may contain errors
Lab products found in correlation
Phospho p38 thr180 tyr182, by Cell Signaling Technology (1 mentions)
Percoll, by GE Healthcare (1 mentions)
Wortmannin, by Merck Group (1 mentions)
Bvd 523, by Selleck Chemicals (1 mentions)
Az628, by Merck Group (1 mentions)
Czc24832, by Merck Group (1 mentions)
β actin, by Abcam (1 mentions)
Pf 3758309, by Selleck Chemicals (1 mentions)
Fr180204, by Selleck Chemicals (1 mentions)
Azd6244, by Selleck Chemicals (1 mentions)
Ipa 3, by Selleck Chemicals (1 mentions)
Phospho mek1 2 ser217 221, by Cell Signaling Technology (1 mentions)
2 protocols using as 252424
1
Rat aortic SMC response to IFN-γ
2
Signaling Pathway Inhibition Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Unless indicated otherwise, all reagents were obtained from Sigma-Aldrich. All reagents were of the highest available grade and lowest possible endotoxin level. Tissue culture media and buffers were obtained from Life Technologies, and dextran and Percoll were from GE Healthcare. The antibodies used were as follows: β-actin (rabbit polyclonal) from Abcam; Bax (clone B-9) and Mcl1 (rabbit polyclonal) from Santa Cruz Biotechnology; phospho-Akt Thr 308 (clone L32A4), phospho-Akt Ser 473 (clone D9E biotinylated), phospho-Erk Thr 202/Tyr 204 (clone E10), phospho-Mek1/2 Ser 217/221 (rabbit polyclonal), phospho-p38 Thr 180/Tyr 182 (rabbit polyclonal), and phospho-Pak1 Ser 144 (rabbit polyclonal) from Cell Signaling Technology; and CD62L-PC5 conjugate from Immunotech.
The inhibitors and final concentrations used were as follows: pan-PI3K, wortmannin (50 nM), and LY2940002 (10 μM, used for prolonged inhibition because wortmannin has a very short half-life in aqueous solutions); PI3Kα and A66 (10 μM); PI3Kβ and TGX221 (40 nM); PI3Kδ and IC87114 (1 μM); and PI3Kγ, AS252424 (30 μM), and CZC24832 (10 μM) (all from Sigma); the Raf inhibitor AZ628 (5 μM); the Mek inhibitors AZD6244 (1 μM) and trametinib (1 μM); the Pak inhibitors PF3758309 (5 μM) and IPA3 (10 μM); the Erk inhibitor FR180204 (10 μM); and BVD523 (5 μM) (all from Selleckchem).
The inhibitors and final concentrations used were as follows: pan-PI3K, wortmannin (50 nM), and LY2940002 (10 μM, used for prolonged inhibition because wortmannin has a very short half-life in aqueous solutions); PI3Kα and A66 (10 μM); PI3Kβ and TGX221 (40 nM); PI3Kδ and IC87114 (1 μM); and PI3Kγ, AS252424 (30 μM), and CZC24832 (10 μM) (all from Sigma); the Raf inhibitor AZ628 (5 μM); the Mek inhibitors AZD6244 (1 μM) and trametinib (1 μM); the Pak inhibitors PF3758309 (5 μM) and IPA3 (10 μM); the Erk inhibitor FR180204 (10 μM); and BVD523 (5 μM) (all from Selleckchem).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!