All cell lines were purchased from ATCC including HeLa (ATCC CCL-2), Raw264.7 (ATCC TIB-71), MDA-MB-231 (ATCC HTB-26), MDA-MB-468(ATCC HTB-132), MCF7 (ATCC HTB-22), 3T3-L1 MBX (ATCC CRL-3242), U-87 MG (ATCC HTB-14). Azido-palmitic acid (1346) and L-Azidohomoalanine (1066) were purchased from Click chemistry tools. D-glucose (1,2,3,4,5,6,6-D7, 97–98%, DLM-2062), algal amino acid mixture (U-13C, 97–99%, CLM-1548), 2-deoxy-D-glucose (U-13C6, 99%, CLM-10466) were purchased from Cambridge isotope laboratories. Deuterium oxide (151882), Taxol (T7402) and Gemcitabine (G6423) were purchased from Sigma-Aldrich. DMEM medium (11965), FBS (10082), penicillin/streptomycin (1514), DMEM medium without L-methionine, L-cysteine and L-glutamine (21013), DMEM without glucose (11966) and proteinase K (EO0491) were purchased from ThermoFisher Scientific. CaF2 substrates (CAFP25–1, CAFP13–1 and CAFP-76–26-1U) were purchased from Crystran.
Dlm 2062
Manufactured by Cambridge Isotopes
The DLM-2062 is a high-performance liquid chromatography (HPLC) system designed for precise chemical analysis. It features a dual-channel detector capable of monitoring multiple wavelengths simultaneously, enabling efficient separation and identification of complex samples. The DLM-2062 is a versatile instrument suitable for a wide range of analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Azido palmitic acid 1346, by Vector Laboratories (2 mentions)
Algal amino acid mixture u 13c, by Cambridge Isotopes (2 mentions)
2 deoxy d glucose u 13c6, by Cambridge Isotopes (2 mentions)
Penicillin streptomycin 1514, by Thermo Fisher Scientific (2 mentions)
Dmem medium without l methionine, by Thermo Fisher Scientific (2 mentions)
L cysteine and l glutamine 21013, by Thermo Fisher Scientific (2 mentions)
Proteinase k eo0491, by Thermo Fisher Scientific (2 mentions)
Raw264, by American Type Culture Collection (2 mentions)
3t3 l1 mbx, by American Type Culture Collection (2 mentions)
Gemcitabine g6423, by Merck Group (2 mentions)
Dmem without glucose, by Thermo Fisher Scientific (2 mentions)
L azidohomoalanine, by Vector Laboratories (2 mentions)
D glucose, by Cambridge Isotopes (2 mentions)
Mda mb 468, by American Type Culture Collection (2 mentions)
Mcf 7, by American Type Culture Collection (2 mentions)
Mda mb 231, by American Type Culture Collection (2 mentions)
U87mg, by American Type Culture Collection (2 mentions)
3 protocols using dlm 2062
1
Multiomics Analysis of Cell Lines
2
Multiomics Analysis of Cell Lines
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All cell lines were purchased from ATCC including HeLa (ATCC CCL-2), Raw264.7 (ATCC TIB-71), MDA-MB-231 (ATCC HTB-26), MDA-MB-468(ATCC HTB-132), MCF7 (ATCC HTB-22), 3T3-L1 MBX (ATCC CRL-3242), U-87 MG (ATCC HTB-14). Azido-palmitic acid (1346) and L-Azidohomoalanine (1066) were purchased from Click chemistry tools. D-glucose (1,2,3,4,5,6,6-D7, 97–98%, DLM-2062), algal amino acid mixture (U-13C, 97–99%, CLM-1548), 2-deoxy-D-glucose (U-13C6, 99%, CLM-10466) were purchased from Cambridge isotope laboratories. Deuterium oxide (151882), Taxol (T7402) and Gemcitabine (G6423) were purchased from Sigma-Aldrich. DMEM medium (11965), FBS (10082), penicillin/streptomycin (1514), DMEM medium without L-methionine, L-cysteine and L-glutamine (21013), DMEM without glucose (11966) and proteinase K (EO0491) were purchased from ThermoFisher Scientific. CaF2 substrates (CAFP25–1, CAFP13–1 and CAFP-76–26-1U) were purchased from Crystran.
3
Glucose Cycling in Beta Cell Lines
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Glucose cycling in βTC3 and INS1-832/13 cells was measured using our previously defined approach (8 (link)). Briefly, 8 to 10 million cells were incubated for 24 or 72 h at 37 °C in RPMI 1640 medium containing 5 mM or 11 mM glucose in 10-cm dishes. Cells were incubated in either naturally labeled glucose or [1,2,3,4,5,6,6-2H7]glucose (Cambridge Isotope Laboratories; Cat no. DLM-2062). Following the 24- or 72-h incubation, the supernatant was collected for glucose derivatization and GC-MS analysis (see Extraction of metabolites and GC-MS analyses ). The glucose MID was quantified, and absolute glucose concentration was determined through comparison to a standard curve. Glucose cycling and glucose uptake rates were calculated as described previously (8 (link)).
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