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Phrodo green staphylococcus aureus bioparticles

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The PHrodo™ Green Staphylococcus aureus Bioparticles are fluorogenic probes designed to detect the phagocytosis of Staphylococcus aureus by cells. The bioparticles are non-fluorescent at neutral pH and become fluorescent upon acidification, such as within phagosomes or lysosomes.

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PHrodo Staphylococcus aureus Bioparticles Staphylococcus aureus BioParticles Staphylococcus aureus PHrodo green

2 protocols using phrodo green staphylococcus aureus bioparticles

1

Neutrophil Phagocytic Capacity and Priming Assessment

Cited 3 times
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Neutrophil phagocytic capacity was assessed using two independent methods: a light microscopic analysis of isolated neutrophils using zymosan particles pre-opsonized using autologous serum [10 (link), 11 (link)], and a flow cytometric analysis of whole blood using pHrodo™ Green Staphylococcus aureus Bioparticles® (Thermofisher, MA, USA) [12 (link)]. Neutrophil priming was assessed by quantifying expression of the cell surface markers CD11b and CD62L using a whole blood flow cytometric method as previously described [4 (link), 13 (link)].
Funston W., Ruchaud-Sparagano M.H., Scott J., Powell J., Cooles F.A., Shelmerdine L., McDowell C., O’Leary D., Booth K.L., Clark S.C., Ledingham S.J., Rostron A.J., Dark J.H, & Simpson A.J. (2022). A human model of bilateral pulmonary vein sampling to assess the effects of one-lung ventilation on neutrophil function. PLoS ONE, 17(7), e0271958.
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2

Neutrophil Phagocytosis and Killing

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Phagocytosis by mucus-or blood-derived neutrophils was estimated using flow cytometric quantification of pHrodo® Green Staphylococcus aureus Bioparticles® (Thermofisher, MA, USA) taken up by neutrophils (see online supplement for greater detail and representative flow cytometry plots) (31). Neutrophils were also incubated with live Pseudomonas aeruginosa and bacterial killing estimated as a percentage of P. aeruginosa colony forming units (CFU), determined by serial dilution and incubation on Lennox B agar plates, compared to a no neutrophil control (see online supplement) (32).
Powell J., Garnett J.P., Mather M.W., Cooles F.A.H., Nelson A., Verdon B., Scott J., Jiwa K., Ruchaud-Sparagano M.H., Cummings S.P., Perry J.D., Wright S.E., Wilson J.A., Pearson J., Ward C, & Simpson A.J. (2018). Excess Mucin Impairs Subglottic Epithelial Host Defense in Mechanically Ventilated Patients. American journal of respiratory and critical care medicine, 198(3).
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