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2 protocols using nahseo3

1

Cytotoxicity Evaluation of Cell Lines

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Dulbecco’s Modified Eagle’s Medium (DMEM) and Fungizone (Amphotericin B) were purchased from Gibco (Waltham, MA, USA). Fetal bovine serum (FBS), trypsin, penicillin/streptomycin, and phosphate-buffered saline (PBS) were purchased from Biosera (Boussens, France). MRS Broth, LAB094 was purchased from LabM (Bury, UK). The Annexin V/PI kit was purchased from BD Biosciences (Franklin Lakes, NJ, USA). Acetic acid, trichloroAcetic acid (TCA), Trizma base, sulforhodamine B (SRB), NaHSeO3 and all other chemicals mentioned were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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2

Cell Apoptosis and Oxidative Stress Assays

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Dulbecco's Modified Eagle's Medium (DMEM) and Fungizone (Amphotericin B) were purchased from Gibco (Gaithersburg, MD, USA). Fetal bovine serum (FBS), trypsin, penicillin/streptomycin, and phosphate-buffered saline (PBS) were purchased from Biosera (Boussens, France). MRS Broth, LAB094 and Agar No. 2 (MC006) were purchased from LabM (UK). The Annexin V-PI kit was purchased from BD Biosciences (USA). The Human Apoptosis Antibody Array kit (ARY 009) from R&D systems (USA) was used. Carboxy-H2DCFDA (C400) was purchased from Invitrogen (Invitrogen, USA). Acetic acid, trichloroAcetic acid (TCA), Trizma base, NaHSeO3, H2O2 and sulforhodamine B (SRB) were purchased from Sigma-Aldrich (Germany).
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