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2 protocols using anti caspase 11 antibody

1

Immunoblotting Analysis of Inflammatory Pathways

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Anti-cleaved caspase-3 antibody, anti-caspase-3 antibody, anti-β-actin antibody, anti-RIP1 (receptor-interacting protein 1) antibody, anti-p-RIP1 (phospho-RIP1) antibody, cell lysis buffer (10×), and protease/phosphatase inhibitor cocktail were all from Cell Signaling Technology (Danvers, MA). Anti-caspase-11 antibody, anti-pro caspase-1+p10+p12 antibody, anti-GSDMD antibody, anti-GSDME antibody, and propidium iodide were from Abcam (Cambridge, UK). Anti-GFP antibody, Hoechst 33342, MitoTracker Deep Red FM, and LysoTracker Deep Red were from Invitrogen. Anti-F4/80 antibody was from BD Biosciences (Franklin Lakes, NJ). TMR red kit was from Roche (Basel, Switzerland). Lipofectamine 3000 Transfection Reagent was from Thermo Fisher Scientific.
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2

Caspase-11 Protein Detection

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Cell lysates were separated by SDS-PAGE, transferred to PVDF membranes, and probed with anti-Caspase-11 antibody (Abcam, ab180673), anti-β-Actin antibody (Cell signaling, 4967), anti-Cyclophilin B antibody (Abcam, ab178397). HRP-conjugated anti-Rabbit IgG antibody (Sigma, A0545) was used as secondary antibody. Immunoblots were developed using ECL method and exposed to X-ray film.
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