Eif4g ser1108

Manufactured by Cell Signaling Technology

Sourced in United States

EIF4G (Ser1108) is a primary antibody that recognizes the phosphorylation of eIF4G at serine 1108. eIF4G is a component of the eIF4F complex, which is involved in the initiation of protein translation.

Automatically generated - may contain errors

Lab products found in correlation

Supersignal west femto maximum sensitivity substrate kit, by Thermo Fisher Scientific (2 mentions) Molecular imager chemidoc xrs system, by Bio-Rad (2 mentions) Rvfv mp12 antibody, by IBT Bioservices (2 mentions) P70 s6 kinase thr389, by Cell Signaling Technology (2 mentions) P90rsk ser380, by Cell Signaling Technology (1 mentions) Ab49900, by Abcam (1 mentions)

2 protocols using eif4g ser1108

Protein Expression Analysis by Western Blot

Cited 1 time

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Protein lysates were collected and analyzed by western blot as previously described [42 (link)]. In brief, primary antibodies against p90RSK (Ser380) (Cell Signaling Technology, Danvers, MA, USA, 9341), p70 S6 Kinase (Thr389) (Cell Signaling Technology 9205), S6 ribosomal protein (Ser235/236) (Cell Signaling Technology, 4856), eIF4G (Ser1108) (Cell Signaling Technology, 2441), RVFV MP12 Antibody (IBT Bioservices, Rockville, MD, USA, 04-0001), or HRP-conjugated actin (Abcam, ab49900) were diluted 1:1000 in 5% bovine serum albumin in 1× TBS with 0.1% Tween-20 solution followed by the addition of the appropriate secondary antibody. The western blots were visualized by chemiluminescence using SuperSignal West Femto Maximum Sensitivity Substrate kit (ThermoScientific, Waltham, MA, USA) and a Bio Rad Molecular Imager ChemiDoc XRS system (Bio-Rad, Hercules, CA, USA) [41 (link),42 (link)].

Bell T.M., Espina V., Lundberg L., Pinkham C., Brahms A., Carey B.D., Lin S.C., Dahal B., Woodson C., de la Fuente C., Liotta L.A., Bailey C.L, & Kehn-Hall K. (2018). Combination Kinase Inhibitor Treatment Suppresses Rift Valley Fever Virus Replication. Viruses, 10(4), 191.

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Western Blot Analysis of Signaling Proteins

Cited 1 time

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Protein lysates were collected and analyzed by western blot as previously described (Austin et al., 2012 (link)). In brief, primary antibodies against p70 S6 Kinase (Thr389) (Cell Signaling Technology 9205), S6 ribosomal protein (Ser235/236) (Cell Signaling Technology, 4856), eIF4G (Ser1108) (Cell Signaling Technology, 2441), RVFV MP12 Antibody (IBT Bioservices 04-0001), or HRP-conjugated actin (Abcam, ab49900) were diluted 1:1000 in 5% bovine serum albumin in 1× TBS with 0.1% Tween-20 solution followed by the addition of the appropriate secondary antibody. The western blots were visualized by chemiluminescence using SuperSignal West Femto Maximum Sensitivity Substrate kit (ThermoScientific) and a Bio Rad Molecular Imager ChemiDoc XRS system (Bio-Rad) (Austin et al., 2012 (link); Baer et al., 2012 (link)).

Bell T.M., Espina V., Senina S., Woodson C., Brahms A., Carey B., Lin S.C., Lundberg L., Pinkham C., Baer A., Mueller C., Chlipala E.A., Sharman F., de la Fuente C., Liotta L, & Kehn-Hall K. (2017). Rapamycin modulation of p70 S6 kinase signaling inhibits Rift Valley fever virus pathogenesis. Antiviral research, 143, 162-175.

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