Western blot and immunoprecipitations were performed by standard method using the following antibodies: monoclonal mouse anti-human β-actin (Cell Signaling, Danvers, MA, USA; 4967), HP1γ (sc-365085) antibodies, polyclonal rabbit anti-human PIM-1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA; sc-28777), Ras (Santa Cruz Biotechnology; sc-29), and p16 (Santa Cruz Biotechnology; sc-468) antibodies, polyclonal rabbit anti-human p53 (Cell Signaling; 9282), p21 (Cell Signaling; 2947), p-Rb (Cell Signaling; 9308), Rb (Cell Signaling; 9313), HP1γ(Cell Signaling; 2619), p-STAT3 (Cell Signaling; 9145), STAT3 (Cell Signaling; 4904), HMGA2 (Cell Signaling; 8179), pHP1γS93. Phosphor-specific HP1γ at Ser 93 (pHP1γS93) antibody was prepared as described previously (Lomberk et al., 2006 (link)).
Monoclonal mouse anti human β actin
Manufactured by Cell Signaling Technology
Sourced in United States
Monoclonal mouse anti-human β-actin is a laboratory reagent used to detect the presence and quantity of the β-actin protein in biological samples. β-actin is a ubiquitous cytoskeletal protein found in all eukaryotic cells and is commonly used as a housekeeping gene or loading control in various assays.
Automatically generated - may contain errors
Lab products found in correlation
P stat3, by Cell Signaling Technology (1 mentions)
Anti human p53, by Cell Signaling Technology (1 mentions)
Stat3, by Cell Signaling Technology (1 mentions)
Hmga2, by Cell Signaling Technology (1 mentions)
Cell lytic m, by Merck Group (1 mentions)
Odyssey imaging system, by LI COR (1 mentions)
Irdye 800cw goat anti mouse, by LI COR (1 mentions)
Image studio lite software, by LI COR (1 mentions)
2 protocols using monoclonal mouse anti human β actin
1
Immunoblotting and Immunoprecipitation Protocols
2
Quantifying Src Phosphorylation in BD Cells
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BD cells were treated with either vehicle (0.1%) or dasatinib for 4 h. Cells were lysed using CellLytic M (Sigma-Aldrich) supplemented with 1% protease inhibitor and phosphatase cocktail inhibitor (P8340/P5726 – Sigma-Aldrich). A total of 40 μg of protein per lane were separated by Novex NuPAGE SDS-PAGE (4–12%), and transferred to polyvinylidene difluoride membrane. Membranes were blocked with 5% BSA in TBS buffer, and probed overnight with monoclonal rabbit anti-human antibodies against phospho-SRC (44-660G, Thermo Fisher) or SRC (2109, Cell Signaling), and monoclonal mouse anti-human β-actin (8H10D10, Cell Signaling). Secondary antibodies include IRDye 800CW goat anti-mouse and 680RD goat anti-rabbit (LI-COR). Infrared fluorescence was detected using Odyssey Imaging System (LI-COR), and analyzed using Image Studio™ Lite software (LI-COR).
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