Blood chemistry was determined by an OPTI® CCA‐TS2 blood gas analyser using an E‐Cl Type cassette (OPTIMedical). Blood, urine, gut sac and intestinal content clinical chemistry were performed using commercially available assays that were modified to work with small volumes.48 , 49 The concentration of hormones was determined using the following commercially available assays: PTH (1‐84; Quidel Corporation), intact FGF‐23 (Quidel Corporation). Urinary creatinine was determined using Infinity™ Creatinine Liquid Stable Reagent (Thermo Fisher Scientific). Glucose and Pi concentrations were determined using commercially available assays (Thermo Fisher Scientific Glucose Hexokinase Reagent and Pointe Scientific Phosphorus Reagent, respectively). Na+ was measured by flame photometry (BWB Technologies Ltd.).
Pth 1 84
Manufactured by Quidel
The PTH (1-84) is a lab equipment product that measures the concentration of parathyroid hormone (PTH) in the blood. PTH is a hormone produced by the parathyroid glands that helps regulate calcium and phosphate levels in the body. The PTH (1-84) assay is used to assess parathyroid gland function and diagnose conditions related to calcium and bone metabolism.
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Lab products found in correlation
2 protocols using pth 1 84
1
Comprehensive Blood and Tissue Analysis
2
Serum Biomarker Measurement in Mice
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Blood was collected from anesthetized mice maintained at 2% isoflurane via cardiac puncture and collected in serum separation tubes incubated for 30 min at room temperature. Serum was isolated by centrifugation and subsequent chemistry analysis performed using enzyme-linked immunosorbent assays. Serum intact PTH 1-84 (Quidel Corporation, San Diego, CA) and hepcidin (Elabscience, Houston, TX), levels were measured using a BioTek Synergy HTX Multi-Mode Reader (BioTek Instruments, Inc., Highland Park, MO) at wavelengths specified by manufacturer protocol. Serum phosphate and iron levels were analyzed by the Animal Histopathology and Laboratory Medicine Core, University of North Carolina-Chapel Hill, Chapel Hill, NC. Serum creatinine levels were performed by the UAB O’Brien Center using liquid chromatography-tandem mass spectrometry (UAB-UCSD O’Brien Center Bioanalytical Resource Core, Birmingham, AL).
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