A LC‐30AD UPLC chromatography system (Shimadzu Corporation) coupled with TripleTOF 5600 (SCIEX) was used for injection and analysis of lipids. AnalystR TF 1.6 and MultiQuantTM data acquisition software (AB SCIEX), PeakView 2.0 (AB SCIEX) and LipidView 2.0 (AB SCIEX) data analysis software were used in experiment and data analysis. Two mobile phases A and B solution were prepared according to the formula water:methanol:acetonitrile:300 mM ammonium acetate = 20:20:20:1 (v/v/v/v) and isopropanol:methanol:300 mM ammonium acetate = 180:20:3 (v/v/v), respectively. Mobile phases A and B were sonicated for 30 min using an ultrasonicator before use. All reagents were HPLC grade, and the water was obtained from the Milli‐Q machine (Millipore).
Milli q machine
Manufactured by Merck Group
Sourced in Japan, China, Germany
The Milli-Q machine is a water purification system designed to produce high-quality ultrapure water. It uses a multi-stage filtration process to remove impurities, ions, and contaminants from the input water, resulting in water that meets the highest purity standards.
Automatically generated - may contain errors
Lab products found in correlation
Tripletof 5600, by AB Sciex (1 mentions)
Peakview 2, by AB Sciex (1 mentions)
Microspin g 25 column, by GE Healthcare (1 mentions)
Talon metal affinity resin, by Takara Bio (1 mentions)
Wizard sv gel and pcr clean up system, by Promega (1 mentions)
Restriction endonuclease, by New England Biolabs (1 mentions)
Luria bertani medium, by BD (1 mentions)
Talon disposable gravity column, by Takara Bio (1 mentions)
Pureyield plasmid miniprep system, by Promega (1 mentions)
In fusion hd cloning kit, by Takara Bio (1 mentions)
Pssm 8 peptide synthesizer, by Shimadzu (1 mentions)
Hen egg white lysozyme, by Merck Group (1 mentions)
Toyopearl mx trp 650m, by Tosoh (1 mentions)
Milli q water, by Merck Group (1 mentions)
Sarcosine, by Merck Group (1 mentions)
5 protocols using milli q machine
1
Lipid Profiling by LC-MS/MS
2
Cloning and Purification of scFv Antibody
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Restriction endonuclease was from New England Biolabs Japan (Tokyo, Japan). The In-Fusion HD cloning kit, pGro7 chaperonine plasmid, Talon metal affinity resin, Talon disposable gravity column, and a Brevibacillus In Vivo Cloning (BIC) system were from Takara Bio (Otsu, Japan). The KOD Plus mutagenesis kit was from Toyobo Biochemicals (Osaka, Japan). The PureYield Plasmid Miniprep System and Wizard SV Gel and PCR Clean-Up System were from Promega (Tokyo, Japan). MicroSpin G-25 Columns were from GE Healthcare (Amersham, UK). The Luria-Bertani medium was from Beckton-Dickinson (Tokyo, Japan). Immunoblock was from KAC (Hyogo, Japan). 5-TAMRA C6 maleimide was from AAT Bioquest (Sunnyvale, CA, USA). Oligonucleotides and the gene for 3D5 scFv were synthesized by Eurofins Genomics (Tokyo, Japan). The sequence of primers used is summarized in Table S1 . Ultrapure water was prepared using a Milli-Q machine (Millipore Japan, Tokyo, Japan). Biotinylated His6 peptide was synthesized using a PSSM-8 peptide synthesizer (Shimadzu, Kyoto, Japan). Other chemicals and reagents, unless otherwise indicated, were from Sigma-Aldrich (St. Louis, MO, USA) or Fujifilm-Wako pure chemicals (Osaka, Japan).
3
Chitosan-based Gold Nanoparticle Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chitosan (CS, deacetylation > 95%) with low/medium/high (l-CS, m-CS, h-CS) average viscosity (50–100 mPa·s, 200–400 mPa·s, and >400 mPa·s, respectively) were purchased from Rhawn Reagent Co., Ltd., Shanghai, China. Acetic acid and chloroauric acid trihydrate (HAuCl4·3H2O, > 99.9%) were purchased from Aladdin Reagent Co., Ltd., Shanghai, China. Deionized water (18.2 MΩ·cm−1) was produced by a Millipore Milli-Q machine.
4
Lysozyme Purification and Salt Effects
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All materials used in the present work were the same as used in Kreusser et al. [36 ]. Hen egg white lysozyme (Lys, M = 14.3 kDa) with a purity over 90% was obtained from Sigma‐Aldrich and the mixed‐mode resin Toyopearl MX‐Trp‐650M, a methacrylic polymer‐based resin with a tryptophan ligand and an ion exchange capacity of 0.08–0.15 eq/L as specified by the supplier [42 ], was obtained from Tosoh Bioscience. The salts for the preparation of the buffers, namely, monosodium phosphate (NaH2PO4·2H2O) and disodium phosphate (Na2HPO4·2H2O), and the salts that were studied, namely, sodium chloride (NaCl), sodium sulfate (Na2SO4), ammonium chloride (NH4Cl), and ammonium sulfate ((NH4)2SO4), were of analytical grade. All salts were obtained from Carl Roth, except for ammonium chloride which was obtained from Bernd Kraft. For the adjustment of the pH value of the buffer and salt solutions, 1 N sodium hydroxide (NaOH) and 1 N hydrochloric acid (HCl) were used, which were obtained from Carl Roth. The high‐purity water used as solvent was produced with a Milli‐Q machine from Merck Millipore.
5
Isotopic Glyphosate Labeling for Metabolism
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
2-13C,15N-glyphosate (99 atom% 13C, 98 atom% 15N) and unlabeled glyphosate (> 99% purity) were purchased from Sigma-Aldrich (St. Louis, USA) and used for the incubation experiment. AMPA and sarcosine (purity > 98%) were both obtained from Sigma-Aldrich, and glycine (J.T.Baker™) was obtained from Avantor (Deventer, Netherland). All other chemicals were of analytical grade and purchased from Sigma-Aldrich. MilliQ water (18 MΩ) was produced from a Milli-Q machine (Merck, Darmstadt, Germany). Chemicals used for LC-MS analysis were listed in Text S1, S 1.1.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!