In this study, we assessed the following ten serological assays. The Abbott SARS-CoV-2 IgG (N) (Abbott, Chicago, IL, USA; used at National Health Service [NHS] Lothian, UK) and SARS-CoV-2 IgGII Quant (Abbott; used at NHS Greater Glasgow and Clyde, UK) assays are two-step chemiluminescent microparticle immunoassays (CMIA) designed to detect IgG antibodies against nucleocapsid and the receptor binding domain (RBD), respectively. The LIASON SARS-CoV-2 S1/S2 IgG (DiaSorin, Saluggia, Italy; used at NHS Lothian), LIASON Trimeric S IgG (DiaSorin; used at NHS Highland, UK), and sCOVG RBD IgG (Siemens, Erlangen, Germany; used at NHS Tayside, UK) assays are also two-step CMIA designed to detect IgG antibodies. The Euroimmun SARS-CoV-2 IgG assay (Euroimmun, Lübeck, Germany; used at Scottish National Blood Transfusion Service, UK) is an indirect ELISA that uses the S1 domain of the spike protein as the antigen. The Anti-SARS-CoV-2 N and S assays (Roche, Basel, Switzerland; used at NHS Lanarkshire, UK) and the COV2T assay (Siemens; used at NHS Tayside) are two-step bridging electrochemiluminescent immunoassays that use nucleocapsid or the RBD of the spike protein as antigens. The cPass assay (Genscript, Piscataway, NJ, USA) detects antibodies that block binding of a soluble RBD to an immobilised cellular receptor protein.
Cov2t assay
Manufactured by Siemens
Sourced in United Kingdom
The COV2T assay is a laboratory equipment product designed for the detection of the SARS-CoV-2 virus. It is a diagnostic tool used to identify the presence of the COVID-19 virus in patient samples. The core function of the COV2T assay is to provide accurate and reliable results to aid in the diagnosis of COVID-19 infection.
Automatically generated - may contain errors
2 protocols using cov2t assay
1
Comparative Analysis of SARS-CoV-2 Serological Assays
2
SARS-CoV-2 Serological Antibody Detection
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The presence of SARS-Cov-2 serological markers was determined using the chemiluminescence methodology with a cridine ester (COV2T assay, Siemens Healthineers nr. 11206711, Munich, Germany). The COV2T test is an automated 1-step antigen chemiluminescent sandwich immunoassay for total antibodies against the SARS-CoV-2 virus. The COV2T assay is designed as a qualitative assay and detects the presence of total antibodies against SARS-Cov-2, not differentiating between IgM and IgG. The performance of this assay was assessed on a Atellica IM1300 analyzer (Siemens Healthineers, Erlangen, Germany).
For the method comparison, the anti-SARS-CoV-2 ELISA was used. The sensitivity of the COV2T assay increased gradually with disease progression, reaching 100% (95%CI, 89.7-100.0%) 14 days after PCR positivity. After validation by testing serum samples from patients with current or previous diagnosis of COVID-19, this method demonstrated greater sensitivity and clinical specificity when compared to tests that detect IgM and IgG alone, with 99.8% specificity and 100% sensitivity (23, 24) , after 14 days of positivity with RT-PCR.
For the method comparison, the anti-SARS-CoV-2 ELISA was used. The sensitivity of the COV2T assay increased gradually with disease progression, reaching 100% (95%CI, 89.7-100.0%) 14 days after PCR positivity. After validation by testing serum samples from patients with current or previous diagnosis of COVID-19, this method demonstrated greater sensitivity and clinical specificity when compared to tests that detect IgM and IgG alone, with 99.8% specificity and 100% sensitivity (23, 24) , after 14 days of positivity with RT-PCR.
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