C57BL/6 mice, Pmel transgenic mice carrying TCR gene specific for the mouse homolog (Pmel-17) of human gp100 (28 (link)), parental FVB mice and FVBN202 transgenic mice were purchased from the Jackson Laboratory (Bar Harbor, ME). Melanoma cell line B16-gp100 (8 (link)), mammary tumor MMC line (4 ), and DC1.2 DC line were maintained in DMEM media supplemented with 10% heat-inactivated FBS (Life Technologies, Grand Island, NY), 2 mM L-glutamine, 100 U/ml penicillin, and 100 mg/ml streptomycin. All experimental procedures were conducted according to the protocols approved by the Virginia Commonwealth University Institutional Animal Care and Use Committee (Richmond, VA).
Parental fvb mice
Manufactured by Jackson ImmunoResearch
Sourced in Montenegro
Parental FVB mice are a strain of laboratory mice commonly used in genetic research. They serve as the original stock from which other transgenic or genetically modified mouse lines are derived. The core function of these mice is to provide a baseline or control group for comparison in experiments involving genetic modifications.
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Lab products found in correlation
2 protocols using parental fvb mice
1
Murine Melanoma and Breast Cancer Models
2
Murine Models for Tumor Immunology
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C57BL/6, T bet knock-out B6.129S6-Tbx21 tm1Glm (T-bet -), female Balb/c and parental FVB mice were purchased from Jackson Laboratories ((Bar Harbor, ME)). Female Her2/neu transgenic FVBN202 mice were obtained from Charles River Laboratories (Wilmington, MA). FVBN202 mice over-express a non-activated rat neu transgene under the regulation of the mouse mammary tumor virus promoter (28) (link). T-bet -mice are lack of T-box transcription factor, TBX21 (29) (link). C57BL/6 and T-bet -mice were used to study ex vivo and in vivo proliferation of cytotoxic CD8 T cells. Female FVB and FVB202 mice were used to establish MMC tumor model whereas female Balb/c mice were utilized to establish 4T1-luc orthotopic breast tumor model.
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