Serum levels of alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were measured with assay kits for ALT and ALP (Catachem, Bridgeport, CT), respectively. Hepatic contents of triacylglycerol (TG) and non-esterified fatty acids (NEFA) were quantified using Wako TG kit and NEFA kit (Wako Chemicals USA Inc.) as described elsewhere [7 (link)–10 (link)]. Hepatic levels of PC, DG, and malondialdehyde (MDA) were measured by Phosphatidylcholine assay kit, Diacylglycerol assay kit, and OxiSelect™ TBARS assay kit, respectively (Cell Biolabs, Inc., San Diego, CA). Hepatic phospholipase D (PLD) activity was determined by use of a colorimetric assay kit purchased from BioVision (Milpitas, CA). Mitochondial fraction was isolated by differential centrifugation of sucrose and hydrogen peroxide (H2O2) and glutathione (GSH) measured using PeroxiDetect kit and glutathione assay kit, respectively (Sigma-Aldrich, Inc., St. Louis, MO) [9 (link)]. Measurement of protein concentrations was carried out using BCA™ protein assay kit (Thermo Scientific, Rockford, IL).
Diacylglycerol assay kit
Manufactured by Cell Biolabs
The Diacylglycerol assay kit is a quantitative colorimetric assay designed to measure diacylglycerol levels in biological samples. The kit provides a direct and sensitive method for the detection of diacylglycerol.
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Lab products found in correlation
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Colorimetric assay kit, by Abcam (1 mentions)
Phosphatidylcholine assay kit, by Cell Biolabs (1 mentions)
Nefa kit, by Fujifilm (1 mentions)
Glutathione assay kit, by Merck Group (1 mentions)
Wako tg kit, by Fujifilm (1 mentions)
Peroxidetect kit, by Merck Group (1 mentions)
Oxiselect tbars assay kit, by Cell Biolabs (1 mentions)
2 protocols using diacylglycerol assay kit
1
Hepatic Lipid and Oxidative Marker Assays
2
Diacylglycerol Quantification in Organoids
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Diacylglycerol assay kit (Cell Biolabs, cat. no. MET-5028) was used to measure diacylglycerol content according to manufacturer's introduction. Organoids were harvested and sonicated on ice to extract diacylglycerol. Then samples were transferred to a 96-well microtiter plate. Reagents were added and incubated at room temperature for 10 min. Absorbance was measured by fluorescence plate reader at the excitation of 530/25 nm and emission of 590/35 nm. The final results were normalized to total protein concentration of the organoids.
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