Monoclonal mouse anti-gB (Abcam, ab6499) was biotinylated with an EZ-Link sulfo-NHS-biotinylation kit (Thermo Scientific). Avidin-decorated nanoparticles (100 μL, 72 nM) were incubated with 10 μL of biotinlylated anti-gB for 1 h. After washing three times with PBST, 1 μL of the nanoparticle suspension (140 nM) was spotted on the paper reservoir modified with 30% PSS. The capture antibody (mouse monoclonal anti-gB Abcam ab54023) was spotted on the receiving paper substrate (2.5 μL, diluted 1:100 in PBS) and left to dry at room temperature. Control biosensors were prepared in the same way with anti-Escherichia coli (Invitrogen PA1-7213), which does not recognize gB This website uses cookies to improve your user experience. By continuing to use the site, you are accepting our use of cookies. Read the ACS privacy policy.
Ab6499
Manufactured by Abcam
Ab6499 is a laboratory product. It is a protein-based reagent.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 710, by Zeiss (2 mentions)
Ez link sulfo nhs biotinylation kit, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Leica (1 mentions)
Prolong gold containing dapi, by Thermo Fisher Scientific (1 mentions)
Las x core offline version, by Leica (1 mentions)
Ab31624, by Abcam (1 mentions)
3 protocols using ab6499
1
Viral Protein Detection Using Biotinylated Antibodies
2
Subcellular Localization of HCMV Proteins
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MRC5 cells were grown on 12 mm glass coverslips (no. 1.5, Menzel) in 24-well plates. At the indicated timepoints cells were fixed, permeabilized and blocked as for ‘Infectivity assay of gradient fractions’. Mouse anti-HCMV UL99 (Clone 10B4 (21)), mouse anti-HCMV gB, (Abcam ab6499), rabbit anti-GM130 (Abcam ab52649), mouse anti-GM130 (BD Biosciences 610822) and rabbit anti-VAMP3 (Abcam ab200657) primary antibodies were diluted 1:500 in block solution, and coverslips incubated for 60 min at RT, washed three times in PBST, and corresponding anti-mouse or anti-rabbit Alexa Fluor 568 or 633 (Invitrogen) secondary antibodies (1:1000) added for 30 min at RT. Coverslips were washed, and mounted on slides with ProLong Gold containing DAPI (Molecular Probes). Samples were stored at 4°C and protected from light until imaging. Confocal images were acquired using an inverted Leica SP5 using 63x UV corrected oil immersion objective and sequential scan settings of individual channels (Fluor 633, Fluor 568, eGFP and DAPI) with a line average of 4 and frame average 1. Images were viewed and analyzed in LAS X core offline version (Leica).
3
HCMV Protein Localization in HTMCs
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HTMCs were infected with HCMV at MOI of 5 and stained for HCMV proteins with mouse anti-HCMV monoclonal antibody against IE-1 (MAB8129, Millipore) or pp65 (ab31624, Abcam), or glycoprotein B (gB; ab6499, Abcam). IE1, pp65, and gB were made visible by incubation with Alexa 488 conjugated-secondary antibody and stained for actin cytoskeleton using phalloidin. Images were photographed with a confocal microscope (LSM710, Zeiss).
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