HT-1080 cells were collected and washed in cold 1X PBS and nuclear extract was isolated using nuclear extract kit (Cell Extract from Sigma) as per manufacturer protocol. For immunoprecipitation experiments 500 µg of nuclear extract was incubated for 4 hours at 4 °C with 5 µg of anti-TRF2 antibody (Novus Biological) immunoprecipitation was performed using Catch and Release co-immunoprecipitation kit (Millipore) as per manufacturer’s protocol using anti-LSD1 antibody (Cell Signalling Technology) and anti-RAP1 antibody (Santacruz biotechnology). For reverse CoIP, 5 µg of LSD1 was used for immunoprecipitation.
Anti rap1 antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Anti-RAP1 antibody is a protein-based research tool designed to detect the presence and distribution of the RAP1 protein in biological samples. RAP1 is a critical component of the telomere complex, playing a key role in regulating telomere length and function. This antibody can be utilized in various immunoassay techniques, such as Western blotting, immunohistochemistry, and immunofluorescence, to study the expression and localization of RAP1 in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Anti lsd1 antibody, by Cell Signaling Technology (1 mentions)
Catch and release co immunoprecipitation kit, by Merck Group (1 mentions)
Anti trf2 antibody, by Novus Biologicals (1 mentions)
Histofine simple stain dab solution, by Nichirei Biosciences (1 mentions)
Histofine simple stain max po multi, by Nichirei Biosciences (1 mentions)
2 protocols using anti rap1 antibody
1
TRF2, LSD1, and RAP1 Interactions
2
Immunohistochemistry of Placental Rap1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Placental tissues were obtained from three women undergoing legal miscarriages at 6–10 weeks of pregnancy. The use of these tissues was approved by the Clinical Research Ethics Committee of Tokyo University of Pharmacy and Life Sciences (#1512), and informed consent was provided by all participants (n = 3). Placental tissues were embedded in paraffin and sectioned. For immunohistochemistry experiments, the paraffin‐embedded sections were deparaffinized and rehydrated. After boiling with 10 mM citrate buffer (pH 6.0) for antigen retrieval, the sections were soaked in 3% H2O2 for 30 min and then blocked with 10% normal goat serum diluted in phosphate‐buffered saline. Subsequently, the sections were incubated overnight at 4°C with an anti‐Rap1 antibody (1:200 dilution; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) or normal rabbit IgG as a negative control. The following morning, the sections were incubated with antirabbit IgG Fab labeled with horseradish peroxidase (Histofine Simple Stain MAX‐PO MULTI; Nichirei, Tokyo, Japan) and developed with Histofine Simple Stain DAB solution (Nichirei). The nuclei were counterstained with methyl green.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!