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2 protocols using tetramethrin

1

Pyrethroids and Ion Channel Modulation

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Tetramethrin, DM, and PM were obtained from Sigma-Aldrich (Madrid, Spain) and initially diluted in DMSO at 100 mM. For in vitro and in vivo experiments, the stock solution prepared in a glass container was subsequently diluted in the appropriate medium and used at the stated concentrations (0.2 to 100 μM). The final concentration of DMSO was 0.1% or below. Pyrethroids were added to the recording chamber with a pipette to avoid possible sticking of these highly lipophilic compounds to the plastic tubing. Menthol (100 μM), allyl isothiocyanate (AITC; 100 μM) and capsaicin (1 μM), tetraethylammonium (TEA; 1 mM), 4-aminopyridine (4-AP; 1 mM), CdCl2 (100 μM) and CsCl (1 mM), iberiotoxin (50 nM), arachidonic acid (10 μM), cloxyquin (100 μM), thapsigargin (1 μM), and A-803467 (1 μM) were also purchased from Sigma (Madrid, Spain). Tetrodotoxin (1 or 7 μM) was obtained from Alomone Labs (Jerusalem, Israel).
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2

Pesticide Residue Analysis Protocol

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Pesticide reference standards including malathion, diazinon, profenofos, chlorpyrifos, chlorpyrifos-methyl, omethoate, dimethoate, cypermethrin, deltamethrin, fenpropathrin, permethrin, tetramethrin, cyfluthrin, fenvlarate, lamdacyhalothrin , lindane, heptachlor, aldrin, heptachlor epoxide, mixture endosulfan, dieldrin, endrin, o,P-DDT, carbaryl, carbofuran, bendiocarb, atrazine, imidacloprid, thiomethoxam and pendimethalin were purchased from Sigma Aldrich GmbH (Augsburg, Germany), Supelco and Bayer Crop Science , with certified purity ranging from 95% to 99%. Acetonitrile, acetone, sodium chloride, toluene, acetic acid and anhydrous magnesium sulfate were obtained from Scharlau (Barcelona, Spain). QuEChERS Finisterre micro centrifuge tube containing two or more of primary secondary amine (PSA), anhydrous magnesium sulfate (MgSO4) and graphed carbon black (GCB) with details of TR-Q2035 (150mg MgSO4, 25mg PSA, 2.5mg GCB) and TR-Q2015 (150mg MgSO4, 25mg PSA) were obtained from Teknokroma (Barcelona, Spain). Piperonyl Butoxide (PPB) with certified purity 95% used as internal standard was obtained from Sigma Aldrich GmbH.
All the organic solvents used were high performance liquid chromatography (HPLC) grade.
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