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Real envision detection system peroxidase dab kit

Manufactured by Agilent Technologies

The REALTM EnVisionTM Detection System Peroxidase/DAB + kit is a lab equipment product designed for immunohistochemical (IHC) staining. It provides a ready-to-use solution for the detection of target antigens in biological samples using a peroxidase-based chromogenic detection method with 3,3'-diaminobenzidine (DAB) as the chromogen.

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2 protocols using real envision detection system peroxidase dab kit

1

Immunohistochemical Analysis of Obese Tissues

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Serial 5-µm paraffin-embedded sections of obese tissues from WT lean and db/db obese mice were incubated with PTX3 or NPY antibodies (Abcam) and developed using the REALTM EnVisionTM Detection System Peroxidase/DAB + kit (Dako) in accordance with the manufacturer’s protocols as described previously (34 (link)). Tissues were photographed with an Olympus BX51 microscope outfitted with an Olympus DP72 digital camera (Olympus).
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2

Immunohistochemical Analysis of DARC in Mice

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Serial 5-µm-thick sections were cut through tissues from chow diet- or HFD-fed mice and immunohistochemical staining analysis with DARC antibodies (Abcam) was performed utilizing the REALTM EnVisionTM Detection System Peroxidase/DAB+ kit (Dako) according to the manufacturer’s instructions. Tissues were examined and photographed with an Olympus BX51 microscope (Olympus).
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