Rac 030

Mice were intragastrically administrated with a single dose of isaridin E (100 mg/kg) or vehicle at 3 h before anesthesia. Whole blood was drawn from the abdominal aorta and collected in K₂EDTA anticoagulant tubes to evaluate the hematologic parameters with an automatic hematology analyzer (BC-2800 vet, Mindray, Shenzhen, China). The citrated blood was centrifuged at 1500× g (25 °C) for 15 min to obtain the platelet-poor plasma (PPP). PPP was then used to measure the coagulation parameters, including ATPP, PT, TT, and FIB, with an automated coagulation analyzer (RAC-030, Rayto, Shenzhen, China).

Mouse coagulation was measured using a previously described method, with modifications^{43 (link)}. WT and Akip1^−/− mice were infected with Ad-VP35 or Ad-null (3 × 10⁹ PFU) via tail vein injection twice at 24 h intervals. Six days later, the mice were injected with LPS (5 mg/kg) or saline via their tail vein. After 4 h, blood prothrombin time (PT) and serum FIB concentrations were determined using an automated blood coagulation analyzer (Rayto, RAC-030) according to the manufacturer’s instructions.