Blood samples were processed with a LH ELISA, as previously reported (33 (link)). Capture antibody (monoclonal antibody, anti-bovine LHβ subunit, AB_2665514) was purchased from Department of Animal Science at the University of California, Davis. Mouse LH standard (AFP-5306A) and primary antibody (polyclonal antibody, rabbit LH antiserum, AB_2665533) were obtained from Harbour-UCLA (California, USA). Secondary antibody (Horseradish-Peroxidase (HRP)-linked donkey anti-rabbit IgG polyclonal antibody, AB_772206) was purchased from VWR International (Leicestershire, UK). Inter-assay and intra-assay variations were 4.6% and 10.2%, respectively and the assay sensitivity was 0.0015 ng/mL. ODs of the standards were plotted against the log of the standard concentrations, non-linear regression to fit the points and parameters were extracted to calculate the concentration of LH (ng/ml) in blood samples, as previously described (33 (link)). The LH concentration at every time point of blood collection was plotted as a line and scatter graph using Igor Pro 7, Wavemetrics, Lake Oswego, OR, USA. DynPeak algorithm was used for the detection of LH pulses (34 (link)).
Ab 772206
Manufactured by Avantor
Sourced in United Kingdom
The AB_772206 is a laboratory equipment designed for general laboratory use. It is a multipurpose device that can perform a variety of tasks. The core function of this product is to assist in the execution of various experimental procedures and analyses within a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using ab 772206
1
Quantifying Luteinizing Hormone Dynamics
2
Quantification of Luteinizing Hormone
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Processing of blood samples was performed using an LH ELISA as previously reported (32) .
The capture antibody (monoclonal antibody, anti-bovine LHβ subunit, AB_2665514) was purchased from Department of Animal Science at the University of California, Davis. The mouse LH standard (AFP-5306A) and primary antibody (polyclonal antibody, rabbit LH antiserum, AB_2665533) were obtained from Harbour-UCLA (California, USA). The secondary antibody (Horseradish-Peroxidase (HRP)-linked donkey anti-rabbit IgG polyclonal antibody, AB_772206) was purchased from VWR International (Leicestershire, UK). Intraassay and inter-assay variations were 4.6% and 10.2%, respectively. The ODs of the standards were plotted against the log of the standard concentrations. Non-linear regression was used to fit the points and various parameters were extracted to calculate the concentration of LH (ng/ml) in the blood samples, as previously described (32) . The concentration of LH at every time point of blood collection was plotted as a line and scatter graph using Igor Pro 7, Wavemetrics, Lake Oswego, OR, USA. DynPeak algorithm was used for the detection of LH pulses (33) .
The capture antibody (monoclonal antibody, anti-bovine LHβ subunit, AB_2665514) was purchased from Department of Animal Science at the University of California, Davis. The mouse LH standard (AFP-5306A) and primary antibody (polyclonal antibody, rabbit LH antiserum, AB_2665533) were obtained from Harbour-UCLA (California, USA). The secondary antibody (Horseradish-Peroxidase (HRP)-linked donkey anti-rabbit IgG polyclonal antibody, AB_772206) was purchased from VWR International (Leicestershire, UK). Intraassay and inter-assay variations were 4.6% and 10.2%, respectively. The ODs of the standards were plotted against the log of the standard concentrations. Non-linear regression was used to fit the points and various parameters were extracted to calculate the concentration of LH (ng/ml) in the blood samples, as previously described (32) . The concentration of LH at every time point of blood collection was plotted as a line and scatter graph using Igor Pro 7, Wavemetrics, Lake Oswego, OR, USA. DynPeak algorithm was used for the detection of LH pulses (33) .
3
Quantification of Luteinizing Hormone
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were processed with a LH ELISA, as previously reported (33) . Capture antibody (monoclonal antibody, anti-bovine LHβ subunit, AB_2665514) was purchased from Department of Animal Science at the University of California, Davis. Mouse LH standard (AFP-5306A) and primary antibody (polyclonal antibody, rabbit LH antiserum, AB_2665533)
were obtained from Harbour-UCLA (California, USA). Secondary antibody (Horseradish-Peroxidase (HRP)-linked donkey anti-rabbit IgG polyclonal antibody, AB_772206) was purchased from VWR International (Leicestershire, UK). Inter-assay and intra-assay variations were 4.6% and 10.2%, respectively and the assay sensitivity was 0.0015 ng/mL.
ODs of the standards were plotted against the log of the standard concentrations, non-linear regression to fit the points and parameters were extracted to calculate the concentration of LH (ng/ml) in blood samples, as previously described (33) . The LH concentration at every time point of blood collection was plotted as a line and scatter graph using Igor Pro 7, Wavemetrics, Lake Oswego, OR, USA. DynPeak algorithm was used for the detection of LH pulses (34) .
were obtained from Harbour-UCLA (California, USA). Secondary antibody (Horseradish-Peroxidase (HRP)-linked donkey anti-rabbit IgG polyclonal antibody, AB_772206) was purchased from VWR International (Leicestershire, UK). Inter-assay and intra-assay variations were 4.6% and 10.2%, respectively and the assay sensitivity was 0.0015 ng/mL.
ODs of the standards were plotted against the log of the standard concentrations, non-linear regression to fit the points and parameters were extracted to calculate the concentration of LH (ng/ml) in blood samples, as previously described (33) . The LH concentration at every time point of blood collection was plotted as a line and scatter graph using Igor Pro 7, Wavemetrics, Lake Oswego, OR, USA. DynPeak algorithm was used for the detection of LH pulses (34) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!