Blood glucose and lactate were measured immediately after extraction by means of handheld meters: Contour® USB (Bayer HealthCare, UK) and LactatePro™ 2 (Arkray Europe, The Netherlands), respectively. Firstly, an appropriate test strip was inserted into each device (Contour test strips and Lactate Pro 2 test strips, respectively). A small volume of whole blood (∼1 μl) was then extracted from the aliquot and applied to each test strips. After a few seconds, the measurement was displayed on each device screen and was recorded. New test strips were used for each fish. These had been previously validated for Atlantic salmon samples using enzymatic-colorimetric commercial test kits: Glucose (GO) Assay Kit (Sigma) and Lactate Dry-Fast (Sentinel Diagnostics, Italy) [25 (link)].
Contour usb
Manufactured by Bayer
Sourced in United Kingdom
The Contour® USB is a compact and portable blood glucose monitoring device that connects directly to a computer via a USB port. It allows users to easily upload and manage their blood glucose data on a computer.
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Lab products found in correlation
2 protocols using contour usb
1
Rapid Measurement of Blood Glucose and Lactate
2
Validating Biochemical Assays for Atlantic Salmon
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Blood glucose and lactate were measured immediately after extraction by means of handheld meters: Contour® usb (Bayer HealthCare, UK) and LactatePro™ 2 (Arkray Europe, The Netherlands). In addition, to validate these methods for Atlantic salmon, a total of 20 samples were further assayed using enzymatic-colorimetric commercial test kits: Glucose (GO) Assay Kit (Sigma) and Lactate Dry-Fast (Sentinel Diagnostics, Italy). The spectrophotometric method and handheld metre for glucose and lactate analysis were compared by assessing the strength (coefficient of determination; r 2 ) and direction (slope) of the linear relationship between pairs of assay results, for which a regression analysis was performed. Plasma cortisol levels were measured with a commercial ELISA kit (Calbiotech, USA) that was also validated for Atlantic salmon. In all cases, serial dilution of pooled salmon plasma was used to obtain an inhibition curve that, following logit-log transformation, was found to be linear and parallel to the standard curves, indicating that sample and standard glucose, lactate and cortisol were comparable (Soper, 2015) .
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