As described previously11 (link). WB analysis was performed by using the whole-cell lysates (30 μg per well) of the colonic mucosal stripping of TgM9 and their WT littermates’ mice with and without CAC. The primary antibodies used were anti-MMP9 (Cell Signaling, Beverly, MA, USA), anti-γH2AX (Abcam), anti-MDC1 (Novus Biologicals, Littleton, CO), anti-MLH1 (Abcam), anti-MSH2 (Abcam), and anti-PCNA (Abcam). Goat anti-mouse secondary antibody (Bio-Rad, Hercules, CA) or goat anti-rabbit secondary antibody (Abcam) were used. Anti-GAPDH (Abcam) or anti-β actin (Sigma) antibodies were used as the WB loading controls. Bio-Rad Quantity One software was used for densitometry analysis.
Anti mlh1
Manufactured by Abcam
Sourced in United States
Anti-MLH1 is a primary antibody that targets the MLH1 (MutL Homolog 1) protein. MLH1 is a key component of the DNA mismatch repair (MMR) system, which is responsible for recognizing and repairing errors that occur during DNA replication. The Anti-MLH1 antibody can be used to detect and analyze the expression or localization of the MLH1 protein in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti msh2, by Abcam (2 mentions)
Goat anti rabbit secondary antibody, by Abcam (1 mentions)
Goat anti mouse secondary antibody, by Bio-Rad (1 mentions)
Anti mdc1, by Novus Biologicals (1 mentions)
Anti mmp9, by Cell Signaling Technology (1 mentions)
Anti γh2ax, by Abcam (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Quantity one software, by Bio-Rad (1 mentions)
Anti gapdh, by Abcam (1 mentions)
Anti pcna, by Abcam (1 mentions)
2 protocols using anti mlh1
1
Colon Cancer Biomarker Analysis Protocol
2
Immunohistochemical Protein Expression Analysis
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IHC for MLH1, MSH2, XRCC5 (Ku80), Polκ, p53 and ki67 was carried out according to MacDonald et al. (22) . The sections were incubated with the following primary antibodies, all purchased from Abcam: anti-MLH1 (1:100), anti-MSH2 (1:200), anti-XRCC5 (1:200), anti-DNA Polymerase Kappa (1:300), anti-p53 (1:250) and anti-Ki67 (1:100) and then incubated with appropriate secondary antibodies (Spring). Diaminobenzidine (DAB) was used as chromogen and the sections were counterstained with haematoxylin. Five hot spot elds containing at least 200 cells were captured and the positive cells were counted using ImageJ software (National Institutes of Health, Bethesda, MD). Protein expression was evaluated using QuickScore (QS) and two observers scored all samples independently and blinded (22) .
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