K2edta plasma tubes

Manufactured by BD

Sourced in United Kingdom

K2EDTA plasma tubes are used for the collection and preservation of blood plasma samples. They contain the anticoagulant K2EDTA, which prevents blood clotting during the sample collection process.

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K2EDTA (197 citations) K2EDTA tubes (66 citations)

4 protocols using k2edta plasma tubes

Erythrocyte PBUT Levels in HD

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Blood samples from 6 HD patients were collected predialysis to determine the presence of PBUTs in erythrocytes, while blood was sampled from 8 healthy controls and another 6 HD patients (predialysis) to be used in the loading and unloading experiments, respectively. All blood was sampled in K₂EDTA plasma tubes (Becton Dickinson, Plymouth, UK).
This study was conducted according to the Declarations of Helsinki, was approved by the Ethics Committee of Ghent University Hospital (2017/0162), and all participants gave their written informed consent.

Deltombe O., Glorieux G., Marzouki S., Masereeuw R., Schneditz D, & Eloot S. (2019). Selective Transport of Protein-Bound Uremic Toxins in Erythrocytes. Toxins, 11(7), 385.

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Plasma Collection for Biobank Research

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All blood samples were collected just prior to colonoscopy according to a validated Standard Operating Procedure, by trained employees to minimize of preanalytical variation. Blood draw was performed from an antecubital vein into 10 mL K2 EDTA plasma tubes (Becton-Dickinson Vacutainer, NJ). The tubes were centrifuged at 3,000 xg for 10 min at 21°C, leaving plasma, buffy coats and erythrocytes separated. Plasma were transferred to a 50 mL Nunc centrifugation tube and re-centrifuged at 3,000 g for additional 10 min at 21°C. Subsequently, plasma was transferred to MAXXLINE cryo tubes with preprinted unique barcodes (Dacos A/S, Esbjerg, Denmark) and frozen at -80°C. All samples were marked with unique barcodes for easy storage identification, which was performed using FreezerWorks PC-based tracking system (FreezerWorks, Seattle, WA). The entire procedure was completed within 2 hours.

Petersen M.M., Kleif J., Jørgensen L.N., Hendel J.W., Seidelin J.B., Madsen M.R., Vilandt J., Brandsborg S., Rasmussen J.S., Andersen L.M., Khalid A., Ferm L., Gawel S.H., Martens F., Andersen B., Rasmussen M., Davis G.J., Christensen I.J, & Therkildsen C. (2023). Optimizing Screening for Colorectal Cancer: An Algorithm Combining Fecal Immunochemical Test, Blood-Based Cancer-Associated Proteins and Demographics to Reduce Colonoscopy Burden. Clinical colorectal cancer, 22(2).

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TMAO Measurement in Blood Samples

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Blood was collected from 20 donors into both NMR LipoProfile^® test serum separator tubes (Lipotube, Greiner Bio-One, Monroe, NC) and K₂EDTA plasma tubes (BD Diagnostics, Durham, NC). Blood in Lipotubes (serum) was allowed to clot at room temperature for 30 min. Blood in K₂EDTA tubes (plasma) was centrifuged within minutes after multiple gentle tube inversion. The tubes were spun at 3,000 RPM for 15 min at room temperature to separate the serum or plasma. For each tube type, 2 donor specimens (out of 20) were spiked with 20 and 40 μM TMAO to cover points in a broad concentration range. Specimens were aliquoted into 2 mL vials and promptly stored at −80°C until further analysis. TMAO measurements were performed in duplicate for all specimens.

Garcia E., Wolak-Dinsmore J., Wang Z., Li X.S., Bennett D.W., Connelly M.A., Otvos J.D., Hazen S.L, & Jeyarajah E.J. (2017). NMR Quantification of Trimethylamine-N-oxide in Human Serum and Plasma in the Clinical Laboratory Setting. Clinical biochemistry, 50(16-17), 947-955.

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Plasma Isolation and Cryopreservation

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Blood was collected by venipuncture into K2EDTA plasma tubes (BD, Franklin Lakes, NJ), and centrifuged for 10 min at 1,500 x g at room temperature. Plasma was aliquoted in 500 µl volumes into cryotubes, snap frozen using liquid nitrogen within 2 h of blood collection, and stored at -80 °C. Before downstream processing was performed, samples were thawed briefly in a RT water bath with intermittent periods of cooling on ice to prevent sample warming above 0–4 °C. Thawed samples were centrifuged for 10 min at 12,000 x g at 4 °C, aliquoted into smaller volumes (40–100 µl), snap frozen using liquid nitrogen, and stored at -80 °C until used.

Beer L.A., Yin X., Ding J., Senapati S., Sammel M.D., Barnhart K.T., Liu Q., Speicher D.W, & Goldman A.R. (2023). Identification and verification of plasma protein biomarkers that accurately identify an ectopic pregnancy. Clinical Proteomics, 20, 37.

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