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Caspase12 ab62484

Manufactured by Abcam
Sourced in United Kingdom

Caspase12 (ab62484) is a protein that belongs to the caspase family. Caspases are a group of enzymes involved in programmed cell death or apoptosis. Caspase12 (ab62484) is a specific antibody that can be used to detect the expression of the Caspase12 protein in various samples.

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2 protocols using caspase12 ab62484

1

Trx1 Protein and Oxidative Stress Assays

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Human recombinant Trx1 protein (TXN) was purchased from OriGene Technologies, Inc. (TP308876, Rockville, MD, USA), PX-12 was purchased from APExBIO Technology LLC (A4509, Houston, TX, USA), Dihydroethidium (DHE) assay kit was purchased from Bestbio (BB47051, Shanghai, China), ROS assay kit (S0033S) and TUNEL assay kit for cells (C1090) were purchased from Beyotime Tiotechnology (Shanghai, China), In Situ Cell Death Detection kit was purchased from Roche Applied Science (11684795910, Basel, Switzerland), CHOP (#2895) and Trx1 (#2429) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), GRP78 antibody was purchased from GeneTex Inc (GTX113340, Irvine, CA, USA), TXNIP (ab188865), JNK (ab179461), Phosoho-JNK (ab124956) and Caspase12 (ab62484) antibodies were purchased from Abcam (Cambridge, UK), Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Los Angeles, CA, USA).
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2

Neuroprotective Effects of Bioactive Compounds

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CAT and verbascoside (VER) were provided by (MedChemExpress United States) with a purity of 98.04% and 99.83%, leonuride (LEO) was provided by Topscience (China) with a purity of 99.15%, and the product was tested and complied with the given specifications. Dulbecco’s modified Eagle’s medium and fetal bovine serum (FBS) were supplied by Invitrogen (Carlsbad, CA, United States). Primary antibodies including NeuN (ab104224), CHOP (ab11419), GRP78 (ab21685), GAP43 (ab78810), Caspase-12 (ab62484), and MAP-2 (ab5392) were supplied by Abcam (Cambridge, United Kingdom). Bax (R22708), Bcl-2 (250198), Caspase3 (383315), and Cleaved-Caspase3 (341034) were supplied by Zenbio (Chengdu, China). The second antibody antirabbit IgG-conjugated Alexa Fluor488 (ab150061), Alexa Fluor647 (ab150063), Alexa Fluor595 (ab150062), and second antibody antimouse IgG-conjugated Alexa Fluor488 (ab150109) were supplied by Abcam (Cambridge, United Kingdom). An enhanced chemiluminescence kit was supplied by Bio-Rad (Hercules, CA, United States).
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