Following the manufacture's protocols of the Yeast maker™ Yeast Transformation System 2 kit (Cat. No.630439, Clontech, USA), the transformants of pSTU2-APP plasmids were used as a positive control. The pPR3-N plasmid was used as a negative control. The pBT3-N-ORF047, pSTU2-APP, pPR3-N plasmids were transformed into NMY51, respectively. Transformants were grown on SD/-Leu, SD/-Trp agar plates at 30 °C for 3–5 d. To check the ORF047 bait plasmid expression in NMY51, one colony from the SD/-Trp plate was inoculated into SD/-Trp broth and grown to 0.6 OD600 at 30 °C, 250 rpm. The total proteins were subsequently extracted from the centrifuged pelleted cells by the Y-PER yeast protein extraction reagent (Thermo, RF-236781). The extracted proteins were separated by 12% SDS-PAGE and electro-blotted onto PVDF membrane (Millipore, Billerica, Massachusetts, USA) for western blot analysis. The ORF047 bait expression was detected with anti-Lex A mouse McAb (Cat. No.SC-390386, Santa Cruz, USA), followed by m-IgGk BP-HRP second antibody (Cat. No.sc-516102 Santa Cruz, USA), with positive signals revealed, all the membranes were imaged using the ChemiDoc XRS + system (Bio-Rad).
M iggk bp hrp second antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The M-IgGκ BP-HRP second antibody is a detection reagent used in various immunoassay techniques. It binds to the constant region of mouse immunoglobulin G kappa (M-IgGκ) and is conjugated to horseradish peroxidase (HRP), which serves as a reporter enzyme. This antibody can be utilized as a secondary detection reagent in applications where the primary antibody is of mouse IgGκ isotype.
Automatically generated - may contain errors
4 protocols using m iggk bp hrp second antibody
1
Yeast Transformation and Protein Expression
2
Yeast Transformation and Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Following the manufacture's protocols of the Yeast maker™ Yeast Transformation System 2 kit (Cat.
No.630439, Clontech, USA), the transformants of pSTU2-APP plasmids were used as a positive control. The pPR3-N plasmid was used as a negative control. The pBT3-N-ORF047, pSTU2-APP, pPR3-N plasmids were transformed into NMY51, respectively. Transformants were grown on SD/-Leu, SD/-Trp agar plates at 30 °C for 3-5 d. To check the expression of ORF047 bait plasmid in NMY51, one colony from the SD/-Trp plate was inoculated into SD/-Trp broth and grown to 0.6 of OD600 at 30 °C, 250 rpm. The total proteins were subsequently extracted from the centrifuged pelleted cells by the Y-PER yeast protein extraction reagent (Thermo, RF-236781). The extracted proteins were separated by 12% SDS-PAGE and electro-blotted onto PVDF membrane (Millipore, Billerica, Massachusetts, USA) for western blot analysis.
The ORF047 bait expression was detected with anti-Lex A mouse McAb (Cat. No.SC-390386, Santa Cruz, USA), followed by m-IgGk BP-HRP second antibody (Cat. No.sc-516102 Santa Cruz, USA), with positive signals revealed, all the membranes were imaged using the ChemiDoc XRS + system (Bio-Rad).
No.630439, Clontech, USA), the transformants of pSTU2-APP plasmids were used as a positive control. The pPR3-N plasmid was used as a negative control. The pBT3-N-ORF047, pSTU2-APP, pPR3-N plasmids were transformed into NMY51, respectively. Transformants were grown on SD/-Leu, SD/-Trp agar plates at 30 °C for 3-5 d. To check the expression of ORF047 bait plasmid in NMY51, one colony from the SD/-Trp plate was inoculated into SD/-Trp broth and grown to 0.6 of OD600 at 30 °C, 250 rpm. The total proteins were subsequently extracted from the centrifuged pelleted cells by the Y-PER yeast protein extraction reagent (Thermo, RF-236781). The extracted proteins were separated by 12% SDS-PAGE and electro-blotted onto PVDF membrane (Millipore, Billerica, Massachusetts, USA) for western blot analysis.
The ORF047 bait expression was detected with anti-Lex A mouse McAb (Cat. No.SC-390386, Santa Cruz, USA), followed by m-IgGk BP-HRP second antibody (Cat. No.sc-516102 Santa Cruz, USA), with positive signals revealed, all the membranes were imaged using the ChemiDoc XRS + system (Bio-Rad).
3
Yeast Transformation and Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following the manufacture's protocols of the Yeast maker™ Yeast Transformation System 2 kit (Cat. No.630439, Clontech, USA), the transformants of pSTU2-APP plasmids were used as a positive control.
The pPR3-N plasmid was used as a negative control. The pBT3-N-ORF047, pSTU2-APP, pPR3-N plasmids were transformed into NMY51, respectively. Transformants were grown on SD/-Leu, SD/-Trp agar plates at 30 °C for 3-5 d. To check the ORF047 bait plasmid expression in NMY51, one colony from the SD/-Trp plate was inoculated into SD/-Trp broth and grown to 0.6 OD600 at 30 °C, 250 rpm. The total proteins were subsequently extracted from the centrifuged pelleted cells by the Y-PER yeast protein extraction reagent (Thermo, RF-236781). The extracted proteins were separated by 12% SDS-PAGE and electroblotted onto PVDF membrane (Millipore, Billerica, Massachusetts, USA) for western blot analysis.
The ORF047 bait expression was detected with anti-Lex A mouse McAb (Cat. No.SC-390386, Santa Cruz, USA), followed by m-IgGk BP-HRP second antibody (Cat. No.sc-516102 Santa Cruz, USA), with positive signals revealed, all the membranes were imaged using the ChemiDoc XRS + system (Bio-Rad).
The pPR3-N plasmid was used as a negative control. The pBT3-N-ORF047, pSTU2-APP, pPR3-N plasmids were transformed into NMY51, respectively. Transformants were grown on SD/-Leu, SD/-Trp agar plates at 30 °C for 3-5 d. To check the ORF047 bait plasmid expression in NMY51, one colony from the SD/-Trp plate was inoculated into SD/-Trp broth and grown to 0.6 OD600 at 30 °C, 250 rpm. The total proteins were subsequently extracted from the centrifuged pelleted cells by the Y-PER yeast protein extraction reagent (Thermo, RF-236781). The extracted proteins were separated by 12% SDS-PAGE and electroblotted onto PVDF membrane (Millipore, Billerica, Massachusetts, USA) for western blot analysis.
The ORF047 bait expression was detected with anti-Lex A mouse McAb (Cat. No.SC-390386, Santa Cruz, USA), followed by m-IgGk BP-HRP second antibody (Cat. No.sc-516102 Santa Cruz, USA), with positive signals revealed, all the membranes were imaged using the ChemiDoc XRS + system (Bio-Rad).
4
Yeast Transformation and Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following the manufacture's protocols of the Yeast maker™ Yeast Transformation System 2 kit (Cat.
No.630439, Clontech, USA),the transformants of pSTU2-APP plasmids were used as a positive control. ThepPR3-N plasmid was used as a negative control. The pBT3-N-ORF047,pSTU2-APP, pPR3-N plasmids were transformed into NMY51, respectively. Transformants were grown on SD/-Leu, SD/-Trp agar plates at 30°C for 3-5 d. To check the ORF047 bait plasmid expression in NMY51, one colony from the SD/-Trp plate was inoculated into SD/-Trp broth and grown to 0.6 OD600 at 30°C, 250 rpm. The total proteins were subsequently extracted from the centrifuged pelleted cells by the Y-PER yeast protein extraction reagent(Thermo,RF-236781). The extracted proteins were separated by 12% SDS-PAGE and electro-blotted onto PVDF membrane (Millipore, Billerica, Massachusetts, USA) for western blot analysis. The ORF047bait expressionwas detected with anti-Lex A mouse McAb (Cat. No.SC-390386, SantaCruz, USA),followed by m-IgGk BP-HRP second antibody (Cat. No.sc-516102 SantaCruz, USA), with positive signals revealed, all the membranes were imaged using the ChemiDoc XRS + system (Bio-Rad).
No.630439, Clontech, USA),the transformants of pSTU2-APP plasmids were used as a positive control. ThepPR3-N plasmid was used as a negative control. The pBT3-N-ORF047,pSTU2-APP, pPR3-N plasmids were transformed into NMY51, respectively. Transformants were grown on SD/-Leu, SD/-Trp agar plates at 30°C for 3-5 d. To check the ORF047 bait plasmid expression in NMY51, one colony from the SD/-Trp plate was inoculated into SD/-Trp broth and grown to 0.6 OD600 at 30°C, 250 rpm. The total proteins were subsequently extracted from the centrifuged pelleted cells by the Y-PER yeast protein extraction reagent(Thermo,RF-236781). The extracted proteins were separated by 12% SDS-PAGE and electro-blotted onto PVDF membrane (Millipore, Billerica, Massachusetts, USA) for western blot analysis. The ORF047bait expressionwas detected with anti-Lex A mouse McAb (Cat. No.SC-390386, SantaCruz, USA),followed by m-IgGk BP-HRP second antibody (Cat. No.sc-516102 SantaCruz, USA), with positive signals revealed, all the membranes were imaged using the ChemiDoc XRS + system (Bio-Rad).
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