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2 protocols using phosphor tbk1

1

Emodin and APAP Hepatoprotective Mechanisms

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Emodin (purity > 98%) and APAP (purity > 98%) were obtained from Yuanye Biotech Co., LTD (Shanghai, China). The alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), alkaline phosphatase (ALP), GSH, malondialdehyde (MDA), superoxide Dismutase (SOD), assay kits were purchased from Jiancheng Bioengineering Institute (Nanjing, China). The enzyme-linked immunosorbent assay (ELISA) kits for detecting tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10, and IL-1β were purchased from Bioswamp Biotech Co., LTD (Wuhan, China). transferase-mediated nucleotide nick-end labeling (TUNEL) kit was purchased from Beyotime Biotech Co., LTD (Shanghai, China). Primary antibodies against nuclear factor erythroid 2-related factor 2, (Nrf2, #12721, 1:1000), hemeoxygenase-1 (HO-1, #86806, 1:1000), NLRP3 (#15101, 1:1000), Caspase1 (#24232, 1:1000), IL-1β, Bcl-2 (#3498, 1:1000), Bcl-2-associated X protein (Bax) (#2772, 1:1000), phosphor (P)-TBK1 (#5483, 1:1000), P-IRF3 (#29047, 1:1000), cGAS, STING (#31659, 1:1000), GAPDH (#5174, 1:1000) were bought from Cell Signaling Technology (Danvers, MA, USA). Primary antibodies against CYP2E1 (#19937-1-AP 1: 2000), NAD(P)H quinone dehydrogenase 1 (NQO1, #67240-1-Ig, 1: 2000), was purchased from Protenintech Biotech Co., LTD (Wuhan, China).
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2

Emodin and APAP Hepatoprotective Mechanisms

Check if the same lab product or an alternative is used in the 5 most similar protocols
Emodin (purity > 98%) and APAP (purity > 98%) were obtained from Yuanye Biotech Co., LTD (Shanghai, China). The alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), alkaline phosphatase (ALP), GSH, malondialdehyde (MDA), superoxide Dismutase (SOD), assay kits were purchased from Jiancheng Bioengineering Institute (Nanjing, China). The enzyme-linked immunosorbent assay (ELISA) kits for detecting tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10, and IL-1β were purchased from Bioswamp Biotech Co., LTD (Wuhan, China). transferase-mediated nucleotide nick-end labeling (TUNEL) kit was purchased from Beyotime Biotech Co., LTD (Shanghai, China). Primary antibodies against nuclear factor erythroid 2-related factor 2, (Nrf2, #12721, 1:1000), hemeoxygenase-1 (HO-1, #86806, 1:1000), NLRP3 (#15101, 1:1000), Caspase1 (#24232, 1:1000), IL-1β, Bcl-2 (#3498, 1:1000), Bcl-2-associated X protein (Bax) (#2772, 1:1000), phosphor (P)-TBK1 (#5483, 1:1000), P-IRF3 (#29047, 1:1000), cGAS, STING (#31659, 1:1000), GAPDH (#5174, 1:1000) were bought from Cell Signaling Technology (Danvers, MA, USA). Primary antibodies against CYP2E1 (#19937-1-AP 1: 2000), NAD(P)H quinone dehydrogenase 1 (NQO1, #67240-1-Ig, 1: 2000), was purchased from Protenintech Biotech Co., LTD (Wuhan, China).
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