Dba 2

Manufactured by Japan SLC

Sourced in Japan

The DBA/2 is a laboratory equipment product. It is designed for conducting scientific experiments and analyses. The core function of the DBA/2 is to provide a stable and controlled environment for various research and testing activities.

Automatically generated - may contain errors

Lab products found in correlation

C3h hej, by Japan SLC (2 mentions) Dba 1, by Japan SLC (2 mentions) C57bl 6j, by Japan SLC (2 mentions) Balb c, by Japan SLC (2 mentions) Icr mice, by Japan SLC (1 mentions) C57bl 6 mice, by Japan SLC (1 mentions) C3h he mice, by Japan SLC (1 mentions)

6 protocols using dba 2

Thymectomy-Induced Autoimmune Gastritis in Mice

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Male and female BALB/c, BALB/c-nu/nu and DBA/2 mice were originally
purchased from Japan SLC (Hamamatsu, Japan) and maintained on a commercial diet (CE-2,
Nihon CLEA, ) and mated in our animal facility. All mice had free access to food and tap
water and housed on sterilized chaw-chips in 0.5µm filter-cleaned air,
but not under specific pathogen free conditions, in a temperature-controlled room (25 ±
1°C) with 12 h of light per day. Both sexes of mice were used, because there was no sex
difference in the susceptibility for induction of AIG [26 (link)]. The day of birth was taken as day 0 of age. Three-day-old mice were
thymectomized as previously described [10 (link)].
Briefly, the sternum of infants was cut vertically from the salivary glands to the third
rib. Thymic lobes were removed by vacuum suction, and the chest cavity was closed. The
chest cavities of all animals were analyzed when the mice were sacrificed, and mice having
residual thymi were excluded from the experiments. The diagnosis of AIG was made
serologically and histologically when sacrificed, generally at twelve weeks of age for NTx
mice, when the disease development is almost completed (data not shown). All animal
experiments were performed according to the Guidance for Animal Experiments of Niigata
University.

Fujii M., Suzuki K., Suenaga S., Wakatsuki M., Kushida Y., Touma M, & Hosono M. (2014). A Dominant Trait Linked to Chromosome 1 in DBA/2 Mice for the Resistance to Autoimmune Gastritis Appears in Bone Marrow Cells. Experimental Animals, 63(2), 155-167.

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Maintenance of Specific Pathogen-Free Mice

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Male DBA/2 and ICR mice were purchased from Japan SLC. Mice were maintained under specific pathogen-free (SPF) conditions at 23 ± 1ºC with a constant humidity of 55 ± 5% under a 12-h light/dark cycle, and had free access to food (CE-2) and tap water in accordance with the Guidelines for Experimental Animal Care issued by the Prime Minister' s Office of Japan. Mice were used in the present study at 5 weeks of age. All animal experiments followed the guidelines for laboratory animal experiments by Tokyo University of Pharmacy and Life Sciences (TUPLS), and each of the experimental protocols was approved by the Committee of Laboratory Animal Experiments in TUPLS (P15-42).

Tanaka H., Yanai C., Miura N.N., Ishibashi K.I., Yamanaka D., Ohnishi H., Ohno N, & Adachi Y. (2020). Coronary Vasculitis Induced in Mice by Cell Wall Mannoprotein Fractions of Clinically Isolated Candida Species. Medical mycology journal, 61(3).

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Murine Strain Comparison Study

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Six‐week‐old male C57BL/6J, A/J, BALB/c, C3H/HeJ, FVB, 129/SvJ, DBA/1, DBA/2, and ICR mice were purchased from Japan SLC, Inc. (Shizuoka, Japan). The animals were housed in a specific pathogen‐free animal facility at 23°C with 60% humidity with a 12 hr/12 hr light/dark cycle and had ad libitum access to food and tap water. The handling and care of the animals conformed to guidelines established in compliance with current international laws and policies (NIH Guide for the Care and Use of Laboratory Animals, NIH Publication No. 85‐23, 1985, revised 2011) and were approved by the Institutional Animal Care and Use Committee (IACUC) of Seoul National University (SNU‐120913‐1‐2). All experiments were conducted with an effort to minimize the number of animals used and the suffering caused by the procedures used in the study. All mice of each strain (n = 5 per strain) were sacrificed at 12 weeks and processed for tissue preparation.

Kim J.W., Nam S.M., Yoo D.Y., Jung H.Y., Hwang I.K., Seong J.K, & Yoon Y.S. (2018). Strain‐specific differential expression of astrocytes and microglia in the mouse hippocampus. Brain and Behavior, 8(5), e00961.

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Mouse Models for Cancer Immunotherapy

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Male or female 6‐ to 10‐week‐old DBA/2 and C57BL/6 mice were purchased from Japan SLC. P1A‐specific T‐cell receptor (TCR)‐transgenic mice12 were kindly provided by Dr Yang Liu, and backcrossed with DBA/2 mice in our animal facility. P815 mastocytoma, 3LL Lewis lung carcinoma, and B16F10 melanoma were transfected to express human CD20 in our laboratory, referred to as P815‐hCD20, 3LL‐hCD20, and B16F10‐hCD20, respectively. Culture medium used for T cells, 3LL, 3LL‐hCD20, and B16F10‐hCD20 was RPMI‐1640 supplemented with 10% FBS, 1% penicillin‐streptomycin, 25 mmol/L HEPES, and 50 mmol/L 2‐mercaptoethanol. Culture medium used for P815 and P815‐hCD20 cells was DMEM supplemented with 10% FBS and 1% penicillin‐streptomycin. Antimouse PD‐1 mAb was isolated from the supernatants of hybridoma by using Protein A column.13 All animal procedures were approved by the Institutional Animal Care and Use Committee of Yamaguchi University.

Nakajima M., Sakoda Y., Adachi K., Nagano H, & Tamada K. (2019). Improved survival of chimeric antigen receptor‐engineered T (CAR‐T) and tumor‐specific T cells caused by anti‐programmed cell death protein 1 single‐chain variable fragment‐producing CAR‐T cells. Cancer Science, 110(10), 3079-3088.

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Inbred Mouse Strain Comparison

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Six-week-old male C57BL/6J, A/J, BALB/c, C3H/HeJ, FVB, 129/SvJ, DBA/1, DBA/2, and ICR mice were purchased from Japan SLC Inc. (Shizuoka, Japan). The animals were housed in a specific pathogen-free animal facility at 23°C with 60% humidity, a 12 h/12 h light/dark cycle, with ad libitum access to food and tap water. The handling and care of the animals conformed to guidelines established in compliance with current international laws and policies (NIH Guide for the Care and Use of Laboratory Animals, NIH Publication number 85-23, 1985, revised 1996) and were approved by the Institutional Animal Care and Use Committee (IACUC) of Seoul National University (SNU-120913-1-2). All experiments were conducted with an effort to minimize the number of animals used and the suffering caused by the procedures used in the study.

Kim J.W., Nam S.M., Yoo D.Y., Jung H.Y., Kim I.Y., Hwang I.K., Seong J.K, & Yoon Y.S. (2017). Comparison of Adult Hippocampal Neurogenesis and Susceptibility to Treadmill Exercise in Nine Mouse Strains. Neural Plasticity, 2017, 5863258.

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Mouse Model Experimentation Protocol

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Female BALB/c nu/nu, DBA/2, and C3H/He mice (5–6 weeks old) were purchased from Japan SLC (Hamamatsu, Shizuoka, Japan). All experimental animal protocols were approved by the Committee for Ethics of Animal Experimentation and were in accordance with the Guideline for Animal Experiments at the University of Tokyo.

Uchihashi T., Nakahara H., Fukuhara H., Iwai M., Ito H., Sugauchi A., Tanaka M., Kogo M, & Todo T. (2021). Oncolytic herpes virus G47Δ injected into tongue cancer swiftly traffics in lymphatics and suppresses metastasis. Molecular Therapy Oncolytics, 22, 388-398.

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