Blood samples were obtained after a minimum of 8 hours of fasting, and all laboratory examinations were performed in the Neodin Medical Institute, Seoul, Korea. Serum ferritin and 25-hydroxyvitamin D3(25[OH]D3) were measured using an immunoradiometric assay with an RIA kit (DiaSorinInc., Stillwater, MN) using a 1470 WIZARD γ-counter (PerkinElmer, Turku, Finland). Blood hemoglobin was measured using an XE-2100D (Sysmex, Tokyo, Japan).
Xe 2100d
Manufactured by Sysmex
Sourced in Japan
The XE-2100D is a hematology analyzer developed by Sysmex. It is designed to perform complete blood count (CBC) and white blood cell differential analysis. The XE-2100D utilizes flow cytometry and electrical impedance technology to provide accurate and reliable results for a variety of blood parameters.
Automatically generated - may contain errors
Lab products found in correlation
Automatic analyzer 7600, by Hitachi (4 mentions)
1470 wizard gamma counter, by PerkinElmer (3 mentions)
Xn 9000, by Sysmex (2 mentions)
Cobas 8000, by Roche (2 mentions)
Ria kit, by DiaSorin (1 mentions)
Human free bdnf elisa kit, by R&D Systems (1 mentions)
Labospect 008as, by Hitachi (1 mentions)
25 hydroxy vitamin d 125i ria kit, by DiaSorin (1 mentions)
1470 wizard, by PerkinElmer (1 mentions)
Au5800 analyzer, by Beckman Coulter (1 mentions)
Advia 1650, by Siemens (1 mentions)
Advia 120, by Siemens (1 mentions)
Hlc 723g7, by Tosoh (1 mentions)
Cobas 8000 e801, by Roche (1 mentions)
Stadiometer, by Seca (1 mentions)
Automatic analyzer 7600 210, by Hitachi (1 mentions)
Fuji drichem 4000i, by Fujifilm (1 mentions)
Cobas 6000, by Roche (1 mentions)
Liaison xl, by DiaSorin (1 mentions)
Dri chem 4000i, by Fujifilm (1 mentions)
29 protocols using xe 2100d
1
Measurement of Serum Biomarkers
2
Hematological Parameter Analysis
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Hematological parameters were determined from blood samples collected in standard vacutainer-K3EDTA tubes and analyzed in a clinical laboratory (Diagnostyka, Rzeszów, Poland) within 2 h of collection, using the Sysmex XE-2100D.
3
Anthropometric and Blood Measurements Protocol
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Anthropometric measurements of individuals that participated in the present study were taken by the trained staff members. Participants wore light, indoor clothing without shoes for the measurement of body weight and height, as previously described (11 (link)). The narrowest point between the iliac crest and the lower border of the rib cage was used to determine waist circumference. The following formula of weight/height2 (kg/m2) was used to calculate body mass index. A standard mercury sphygmomanometer (Baumanometer; W.A. Baum Co., Inc., Copiague, NY, USA) was used to measure systolic and diastolic blood pressure in the right arm. Blood pressure measurements were performed twice with a 5 min interval and the average of the two measurements was used for analysis. Blood samples were collected from the antecubital vein of each participant to measure the white blood cell count. The white blood cell count was measured using an automated hematology analyzer (XE-2100D; Sysmex Corporation, Kobe, Japan).
4
Biomarker Measurement Protocol
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Following blood collection by trained staff members, samples were transported to the Central Testing Institute (NeoDin Medical Institute) for further analysis. White blood cell (WBC) counts were decided using laser flow cytometry (Sysmex XE-2100D). Serum cholesterol was measured enzymatically using chemical analyser (Hitachi 7600; Hitachi, Ltd.). BP was measured three times at 5-min intervals using a standard mercury sphygmomanometer (Baumanometer, WA Baum Co.) Body mass index (BMI) was determined through dividing body weight (kg) by squared height (m2).
5
Measuring Serum BDNF and Platelet Counts
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We measured serum and not plasma BDNF concentrations as serum measurements have been used more frequently than plasma measurements in previous studies, which improves comparability of our results (serum concentrations combine blood-borne bound and unbound BDNF, and plasma concentrations display only the unbound proportion; Dinoff et al., 2017 (link)). BDNF was measured in participants with at least four of six successful visits in serum by the human free BDNF ELISA kit (R&D Systems, Minneapolis, MN, United States) according to the instructions of the manufacturer with a dilution factor of 1:3 (standard and sample). All standards, samples, and controls were run once. A sigmoid curve was fitted on the resulting data, and the sample concentrations were calculated by the Dynex DS2 software.
Platelet counts were measured with Sysmex fluorescence flow cytometry (XE-2100D, Sysmex Xtra 2/2008) according to the instructions of the manufacturer and in line with the ICSH reference methods (International Council for Standardization in Hematology).
In a second step, results of serum BDNF concentrations and platelet count were corrected according to the method of Dill and Costill for dehydration (Dill and Costill, 1974 (link); Alis et al., 2015 (link)).
Platelet counts were measured with Sysmex fluorescence flow cytometry (XE-2100D, Sysmex Xtra 2/2008) according to the instructions of the manufacturer and in line with the ICSH reference methods (International Council for Standardization in Hematology).
In a second step, results of serum BDNF concentrations and platelet count were corrected according to the method of Dill and Costill for dehydration (Dill and Costill, 1974 (link); Alis et al., 2015 (link)).
6
Biomarkers for Childhood Chronic Rhinosinusitis
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To find a disease-specific marker, we assessed serum total IgE, aeroallergen specific IgE, total peripheral blood eosinophil counts (TEC), and serum eosinophil cationic protein (ECP) levels in children with CRS at initial presentation. The number of peripheral blood eosinophils was counted with blood samples containing EDTA using an automated hematology analyzer (Model: XE2100 D, Sysmex, Japan). Serum total IgE level was measured using ImmumoCAP tests (Phadia AB, Uppsala, Sweden) according to the manufacturer’s instructions. Serum ECP level was measured using a commercially available fluoroimmunoassay kit (Phadia AB, Uppsala, Sweden), which had a detection limit of less than 2.0 μg/L. Blood sample collection, serum preparation, and serum ECP measurement were performed at the beginning of the study according to the manufacturer’s instructions.
7
Hemoglobin-Based Anemia Assessment
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Blood analysis, including Hb level measurements, was performed and used as an index to determine the level of anemia. Hemoglobin levels were measured by the cyanide-free sodium lauryl sulfate method using XE-2100D (Sysmex, Kobe, Japan). Anemia was defined by the World Health Organization (WHO) as hemoglobin levels of <13 g/dL in men and <12 g/dL in women [31 ].
8
Hematocrit as a CVD Risk Predictor
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The outcome of this study was HCT level (%) retrieved from the health check-up file as a predictor of CVD risk23 (link), 24 (link), 25 (link), 26 (link)). Venous blood samples were collected by professional medical technologist from 9 am to 4 pm. HCT levels were measured by the Flow Cytometry method using semiconductor laser (XE-2100D, Sysmex, Kobe, Japan).
9
Broiler Antioxidant and Blood Profiling
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All broilers had 2 mL of blood drawn from the wing veins prior to slaughter,
which was collected in vacuum tubes containing K3EDTA and a tube free
of heparin for serum analysis. The collected blood samples were centrifuged for
20 minutes at 12,500×g at 4°C. An automatic hematology analyzer
(XE2100D, Sysmex, Kobe, Japan) was used to examine red blood cell (RBC), white
blood cell (WBC), heterophil, and lymphocyte samples. For total antioxidant
status (TAS) analysis, the serum samples obtained by centrifugation of the blood
were measured using a total antioxidant capacity assay kit, such as the ELISA
kit (EK780137, AFG Scientific, Northbrook, IL, USA). The uric acid levels were
measured using the Qualigent UA (Qualigent UA, SEKISUI Medical, Tokyo, Japan)
reagent through an enzymatic assay method (Labospect008AS, Hitachi, Tokyo,
Japan).
which was collected in vacuum tubes containing K3EDTA and a tube free
of heparin for serum analysis. The collected blood samples were centrifuged for
20 minutes at 12,500×g at 4°C. An automatic hematology analyzer
(XE2100D, Sysmex, Kobe, Japan) was used to examine red blood cell (RBC), white
blood cell (WBC), heterophil, and lymphocyte samples. For total antioxidant
status (TAS) analysis, the serum samples obtained by centrifugation of the blood
were measured using a total antioxidant capacity assay kit, such as the ELISA
kit (EK780137, AFG Scientific, Northbrook, IL, USA). The uric acid levels were
measured using the Qualigent UA (Qualigent UA, SEKISUI Medical, Tokyo, Japan)
reagent through an enzymatic assay method (Labospect008AS, Hitachi, Tokyo,
Japan).
10
Broiler blood analysis protocol
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Blood samples were collected from the brachial wing vein at 2 and 4 weeks
(before slaughter), 8 broilers per treatment. Blood samples were collected
into vacuum tubes containing K3EDTA for completed blood count
analysis and nonheparinized tubes for serum analysis, respectively. After
collection, serum samples were centrifuged at 12,500×g at 4°C
for 20 min. Red blood cell (RBC), white blood cell (WBC), and lymphocyte
were analyzed using an automatic hematology analyzer (XE2100D, Sysmex, Kobe,
Japan). Total protein (TP) level was measured using a colorimetric method,
and blood urea nitrogen (BUN) level was analyzed using the urease glutamate
dehydrogenase method. The TP and BUN in blood were measured using a fully
automated chemistry analyzer (Cobas C702, Hofmann-La Roche,
Switzerland).
(before slaughter), 8 broilers per treatment. Blood samples were collected
into vacuum tubes containing K3EDTA for completed blood count
analysis and nonheparinized tubes for serum analysis, respectively. After
collection, serum samples were centrifuged at 12,500×g at 4°C
for 20 min. Red blood cell (RBC), white blood cell (WBC), and lymphocyte
were analyzed using an automatic hematology analyzer (XE2100D, Sysmex, Kobe,
Japan). Total protein (TP) level was measured using a colorimetric method,
and blood urea nitrogen (BUN) level was analyzed using the urease glutamate
dehydrogenase method. The TP and BUN in blood were measured using a fully
automated chemistry analyzer (Cobas C702, Hofmann-La Roche,
Switzerland).
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