Hsc 3

Manufactured by American Type Culture Collection

Sourced in United States, China

The HSC-3 is a high-speed centrifuge designed for various laboratory applications. It is capable of reaching speeds up to 30,000 rpm, providing efficient separation of samples. The HSC-3 features a compact and durable construction, making it a versatile option for diverse laboratory settings.

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Lab products found in correlation

64 protocols using hsc 3

Oral Cancer Cell Lines and Control

Cited 2 times

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CAL 27, HSC-3 (ATCC; Manassas, VA, USA), and Ca9-22 (RIKEN BioResource Research Center; Tsukuba, Ibaraki, Japan) oral cancer cell lines were included. The OC-2 [23 (link)] and OECM-1 [24 (link)] oral cancer cell lines were provided by Dr. Wan-Chi Tsai (Kaohsiung Medical University, Kaohsiung, Taiwan). They were established from gingival (Ca9-22 and OCEM-1), tongue (CAL 27 and HSC-3), and buccal mucosa (OC-2) oral squamous cell carcinoma (OSCC). A non-malignant normal gingival epithelial Smulow–Glickman (S–G) cell line [25 (link),26 ], commonly used for examining the safety of anti-oral cancer drugs [27 (link)], was chosen as the control. Cells were maintained in a 3:2 medium mixture of DMEM and F12 (Gibco, Grand Island, NY, USA), supplemented with 10% fetal bovine serum and P/S antibiotics [28 (link)]. For all flow cytometry experiments, cells were seeded at a density of 4 × 10⁴/well/12-well plate and incubated overnight before drug treatment.

Shiau J.P., Chuang Y.T., Yang K.H., Chang F.R., Sheu J.H., Hou M.F., Jeng J.H., Tang J.Y, & Chang H.W. (2022). Brown Algae-Derived Fucoidan Exerts Oxidative Stress-Dependent Antiproliferation on Oral Cancer Cells. Antioxidants, 11(5), 841.

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Culturing Cancer Cells and Neurons

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Cancer cells: The human oral squamous cell carcinoma cell lines, HSC-3 and Hela-O3, were cultured in Dulbecco’s Modification of Eagle’s Medium (DMEM) with 4.5 g/L glucose, l-glutamine and sodium pyruvate, supplemented with 10% fetal bovine serum (FBS), and cultured at 37 °C in 5% CO₂. HSC-3 was purchased from ATCC and used fewer than 6 months after resuscitation. Hela-O3 was obtained from Dr. Roberto Weigert at the National Cancer Institute and tested for Mycoplasma by PCR (ATCC) prior to use.
Neurons: Mouse trigeminal ganglia were harvested and cultured as previously described^{26 (link)}. Trigeminal ganglia were isolated, transferred into Hank’s Balanced Salt Solution (HBSS) and enzyme-digested by incubation with papain (Worthington), collagenase type II (Worthington), and dispase type II (MB). Dissociated neurons were plated on glass coverslips coated with poly-d-lysine and laminin and maintained at 37 °C at 5% CO₂/95% air in F12 media (Life Technologies) with 5% FBS.

Dang D., Ye Y., Aouizerat B.E., Patel Y.K., Viet D.T., Chan K.C., Ono K., Doan C., Figueroa J.D., Yu G, & Viet C.T. (2020). Targeting the endothelin axis as a therapeutic strategy for oral cancer metastasis and pain. Scientific Reports, 10, 20832.

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Culturing OSCC Cell Lines

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OSCC cell lines (Cal 27 and Hsc-3) were obtained from ATCC (American type culture collection, Manassas, VA, USA). Both cells were grown in DMEM/F12 media with 10% FBS.

Hou Y., Xue F., Fu Y., Feng G., Wang R, & Yuan H. (2021). CLPTM1L Is a Novel Putative Oncogene Promoting Tumorigenesis in Oral Squamous Cell Carcinoma. Cell Transplantation, 30, 09636897211045970.

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Oral Cancer Cell Line Characterization

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Five oral cancer cell lines (Ca9-22, SCC-9, CAL 27, OC-2, and HSC-3) and one non-malignant oral cell line (HGF-1) were purchased from ATCC (Manassas, VA, USA) and Health Science Research Resources Bank (HSRRB) (Osaka, Japan). Oral cancer cell line OECM-1 was kindly provided by Dr. Wan-Chi Tsai (Kaohsiung Medical University, Kaohsiung, Taiwan) [55 (link)]. These cell lines were derived from the gingival (Ca9-22, OCEM-1, and HGF-1), tongue (SCC-9, CAL 27, and HSC-3), and buccal (OC-2) locations. The cultural conditions were as described in [56 (link)]. CellTiter 96 Aqueous One Solution, a mitochondrial enzyme reacting kit, was applied to determine cell viability (Promega, Madison, WI, USA) [55 (link),57 (link)].

Yang K.H., Lin Y.S., Wang S.C., Lee M.Y., Tang J.Y., Chang F.R., Chuang Y.T., Sheu J.H, & Chang H.W. (2021). Soft Coral-Derived Dihydrosinularin Exhibits Antiproliferative Effects Associated with Apoptosis and DNA Damage in Oral Cancer Cells. Pharmaceuticals, 14(10), 994.

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Culturing Human Oral Cancer Cell Lines

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Human oral squamous carcinoma cell lines (FaDu and HSC-3) were received from ATCC (Manas, VA, USA). In addition, Ca9-22 cell line was obtained from Japanese Collection of Research Bioresources Cell Bank (JCRB, Shinjuku, Japan) and cultured in the in Dulbecco’s Modified Eagle Medium (DMEM; Life Technologies, Grand Island, NY, USA): Ham’s F12 Nutrient Mixture (Life Technologies, Grand Island, NY, USA) supplemented with 10% FBS (Invitrogen, Waltham, MA, USA) [28 (link)]. The cells were cultured in 5% CO₂ at 37 °C.

Velmurugan B.K., Lin J.T., Mahalakshmi B., Chuang Y.C., Lin C.C., Lo Y.S., Hsieh M.J, & Chen M.K. (2020). Luteolin-7-O-Glucoside Inhibits Oral Cancer Cell Migration and Invasion by Regulating Matrix Metalloproteinase-2 Expression and Extracellular Signal-Regulated Kinase Pathway. Biomolecules, 10(4), 502.

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Oral Squamous Cell Carcinoma Cell Culture

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The HOK cell line (Sciencell) was cultured in Oral Keratinocyte Medium (ScienCell). Three cell lines SCC9, SCC4, and SCC25 were obtained from (ATCC) and maintained in a 1:1 mixture of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 medium (ATCC) supplemented with 400 ng/ml hydrocortisone [Sigma–Aldrich (SA)], 10% fetal bovine serum (Thermo), and 1% penicillin–streptomycin (SA). Three cell lines UM1 (JCRB), HSC3 (JCRB), and CAL27 (ATCC) were maintained in DMEM (ATCC) supplemented with 10% fetal bovine serum (Thermo) and 1% penicillin–streptomycin (SA). The growth environment of these cells was in an incubator set at 37°C with 5% carbon dioxide.

Su Z., Pan C., Xie H., Ning Y., Li S, & Xiao H. (2022). Downregulation of circLPAR3 inhibits tumor progression and glycolysis by liberating miR‐144‐3p and upregulating LPCAT1 in oral squamous cell carcinoma. Laryngoscope Investigative Otolaryngology, 7(2), 425-436.

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Cell Culture Conditions for Oral Cancer

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The dysplastic oral leukoplakia cell line (DOK) and oral cavity cancer cell lines (Ca9-22, HSC3, OC3 and OECM1; ATCC, Manassas, VA, USA) were used in this study. Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) and DMEM/F12 medium with 10% fetal bovine serum (Hyclone Laboratories Inc., South Logan, UT, USA) and antibiotics, at 37 °C in 5% CO₂.

Wang H.C., Chan L.P., Wu C.C., Chang S.J., Moi S.H., Luo C.W, & Pan M.R. (2021). Silencing DNA Polymerase β Induces Aneuploidy as a Biomarker of Poor Prognosis in Oral Squamous Cell Cancer. International Journal of Molecular Sciences, 22(5), 2402.

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Cultivation of Human Oral SCC Cell Line

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The human oral SCC cell line, HSC-3 (ATCC, Manassas, VA) was cultivated in Dulbecco’s Modification of Eagle’s Medium (DMEM) with 4.5 g/L glucose, l-glutamine and sodium pyruvate, supplemented with 10% fetal bovine serum, 25 μg/mL fungizone, 100 μg/mL streptomycin sulfate, and 100 U/mL penicillin G.

Ye Y., Bae S.S., Viet C.T., Troob S., Bernabé D, & Schmidt B.L. (2014). IB4(+) and TRPV1(+) sensory neurons mediate pain but not proliferation in a mouse model of squamous cell carcinoma. Behavioral and Brain Functions : BBF, 10, 5.

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Culturing Human Oral Cancer Cell Lines

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Human OSCC cell line HSC3, SCC15, SCC25, CAL27, SCC9, and normal oral epithelial cell line CGHNK2 were purchased from ATCC. Cells were cultured in RPMI‐1640 medium with 10% fetal bovine serum (Gibco) and 1% penicillin‐streptomycin (Gibco) with 5% CO₂ at 37°C.

Qiu B., Sun Y., Nie W., Yang Q, & Guo X. (2023). FBXW7 promotes autophagy and inhibits proliferation of oral squamous cell carcinoma. Immunity, Inflammation and Disease, 11(5), e845.

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Establishment of OSCC Cell Line Models

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Human OSCC cell lines CAL27 and SCC4 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). OSCC cell line HSC3 was purchased from the Japanese Collection of Research Bioresources Cell Bank (Ibaraki City, Japan) via Sekisui XenoTech (Kansas City, KS, USA). CAL27 and HSC3 were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, ATCC) supplemented with 10% fetal bovine serum (FBS, Corning Inc., Corning, NY, USA) and 1% penicillin-streptomycin (PS, Thermo Fisher Scientific, Waltham, MA, USA). SCC4 was cultured in DMEM supplemented with 10% FBS, 1% PS, and 400 ng/mL hydrocortisone (Sigma-Aldrich, St. Louis, MO, USA). Stable green fluorescent protein (GFP)- and firefly luciferase-expressing cell lines were generated by transfecting cells with CMV-Luciferase-2A-GFP lentivirus (Amsbio, Cambridge, MA, USA) followed by fluorescence-activated cell sorting for GFP expression. CAL27-GFP, SCC4-GFP, and HSC3-GFP cell lines were cultured in the complete medium of the respective parent cell line. All cells were incubated at 37°C in 5% CO₂.

Hall R.C., Ayat N.R., Qiao P.L., Vaidya A.M., Ma D., Aminoshariae A., Stojanov I, & Lu Z.R. (2020). Preclinical assessment of the effectiveness of magnetic resonance molecular imaging of extradomain-B fibronectin for detection and characterization of oral cancer. Molecular imaging and biology, 22(6), 1532-1542.

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