1 25 oh 2d 125i ria kit

Fasting blood samples of the proband were stored at room temperature for 30 min and centrifuged at 3 000 r·min⁻¹ for 10 min to separate the serum for analysis. Age and sex appropriate reference ranges were obtained from the central laboratory of PUMCH. The levels of serum phosphate (Pi), calcium (Ca), alkaline phosphatase (ALP), and other biochemical parameters were analyzed spectrophotometrically using routine assays available at the central laboratory of PUMCH. Serum 25-hydroxyvitamin D [25(OH)D] and intact parathyroid hormone were determined by an automated Roche electrochemiluminescence system (E170; Roche Diagnostics, Basel, Switzerland), whereas serum 1,25(OH)₂D level was determined by a 1,25(OH)₂D ¹²⁵I RIA kit (DiaSorin, Stillwater, Minnesota, USA) at the central laboratory of PUMCH.

Fasted blood samples were collected and placed at room temperature for 30 min, and then centrifuged at 3000 rpm for 10 min to separate the serum for analysis. Twenty-four-hour urine calcium and the blood biochemical parameters including serum calcium (Ca), serum phosphate (P), serum total ALP were measured spectrophotometrically using routine assays in the central laboratory of PUMCH. Serum intact parathyroid hormone (iPTH) and serum 25-hydroxyvitamin D (25(OH)D) were analyzed by an automated Roche electrochemiluminescence system (E170 Roche Diagnostics, Basel, Switzerland). Serum 1,25-dihydroxyvitamin D [1,25(OH)₂D] level was determined with 1,25(OH)₂D ¹²⁵I RIA Kit (Diasorin Inc., Stillwater, MN, U.S.A.).