The human papillary thyroid (B-CPAP) cancer cells and the human normal thyroid cells (Nthy-ori 3-1) were from the National Collection of Authenticated Cell Cultures (Shanghai, China). The medium was fresh RPMI1640 (Gibco, China) with 10% fetal bovine serum and 1% penicillin and streptomycin (Invitrogen, Carlsbad, CA, United States).
Nthy ori3 1
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
The Nthy-ori3-1 cell line is a human thyroid follicular epithelial cell line derived from normal thyroid tissue. It is maintained in cell culture and serves as a model system for studying thyroid cell biology and function. The core function of the Nthy-ori3-1 cell line is to provide a well-characterized, non-transformed cell line for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions)
Tpc 1, by National Collection of Authenticated Cell Cultures (7 mentions)
Bcpap, by National Collection of Authenticated Cell Cultures (5 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Khm 5m, by National Collection of Authenticated Cell Cultures (2 mentions)
Cal 62, by National Collection of Authenticated Cell Cultures (2 mentions)
Dmem medium, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Merck Group (2 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Mda t41, by American Type Culture Collection (1 mentions)
Pen strep, by Thermo Fisher Scientific (1 mentions)
Stem cell culture medium, by Cyagen (1 mentions)
Trem2 sirna, by GenePharma (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Bht 101, by Thermo Fisher Scientific (1 mentions)
Nthy ori 3 1, by Thermo Fisher Scientific (1 mentions)
Bcpap, by Thermo Fisher Scientific (1 mentions)
Cal 62, by Thermo Fisher Scientific (1 mentions)
14 protocols using nthy ori3 1
1
Culturing human thyroid cell lines
2
Thyroid Cancer Cell Line Cultivation
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Thyroid cancer cell lines, including BCPAP, KTC-1, KHM-5M, HTh7, ACT-1, CAL-62, C643, WRO, and TTA-1, as well as normal human primary thyroid follicular epithelial cell Nthy-ori 3-1, were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). The human thyroid cancer cell line MDA-T41 was purchased from the American Type Culture Collection (Manassas, USA). BCPAP, KHM-5M, MDA-T41, C643, and Nthy-ori 3-1 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS). KTC-1 and WRO cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium supplemented with 10% FBS, 1% nonessential amino acids, and 1% sodium pyruvate. CAL-62, HTh7, ACT-1, and TTA-1 were cultured in Dulbecco's Modified Eagle Medium (DMEM) medium supplemented with 10% FBS. All cells were incubated at 37°C in a 5% CO 2 (carbon dioxide) environment.
3
Culturing Cancer and Stem Cells
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W3, TPC-1 and Nthy-ori 3–1 were purchased from Cell Bank of Chinese Academy of Sciences and were cultured in DMEM medium (Gibco, Carlsbad, CA) containing 10% FBS (Gibco) and 1% pen/strep (Gibco) in a humidified atmosphere at 37 °C with 5% CO2. HUCMSCs were isolated from fresh umbilical cord samples as previously described [17 (link), 18 (link)] and maintained in stem cell culture medium (Cyagen, Guangzhou, China).
4
Culturing Thyroid and PDTC Cell Lines
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One normal thyroid epithelial cell line, Nthy-ori 3-1, was obtained from Cell Bank of Chinese Academy of Sciences (Shanghai, China). Two PDTC cell lines, CAL-62 and BHT-101, were obtained from National Infrastructure of Cell Line Resource (Beijing, China). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, NY, USA) with 10% FBS at 37°C.
5
Transfection of Thyroid Cancer Cells
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Human thyroid cancer SW579 and KTC-1 cells and human normal thyroid epithelial cells Nthy-ori 3-1 were bought from Cell Bank of Chinese Academy of Sciences (Shanghai). Cells were cultivated in a sterile incubating device under 37°C, 5% CO2, and 5% v/v. TREM2 siRNA and its negative control (NC) were constructed by GenePharma (Shanghai, PRC). Blank plasmids were used as controls. SW579 cells at logarithm growth phase were inoculated in 6-well dishes and transfected with control, NC, and TREM2 siRNAs at 70%–80% cell fusion after following the instruction manual of Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.). After 48 h of transfection, total RNA was obtained, and RT-PCR was conducted to demonstrate the efficiency of transfection.
6
Thyroid Cancer Cell Lines and Tissues
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Human thyroid cancer cell lines CAL-62, KHM-5M, BHT-101, B-CPAP, and normal thyroid cell lines Nthy-ori-3-1 were purchased from the China Center for Type Culture Collection (CCTCC, China). The CAL-62 and BHT-101 cells were cultured in DMEM medium supplemented with 10% and 20% fetal bovine serum (Gibco, USA) at 37°C in 5% CO2, respectively. KHM-5M, B-CPAP and Nthy-ori-3-1 were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (Gibco, USA) at 37°C in 5% CO2. A total 15 of DTC patients who underwent radical thyroidectomy in Ruijin Hospital of Shanghai Jiaotong University Medical College and were confirmed as DTC by postoperative histopathological examination were included in this study. All samples were obtained with the patients’ informed consent, and the samples were histologically confirmed by at least 2 pathologists independently in a double-blinded fashion.
7
Culture of Thyroid Cell Lines
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Thyroid gland epithelial cell line Nthy-ori3-1 and PTC cell lines TPC-1, IHH-4 and BCPAP were purchased from CCTCC (China Center for Type Culture Collection, Shanghai, China). These cells were cultured with RPMI 1640 medium (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) at 37°C in an atmosphere of humidified air containing 5% CO2.
8
Thyroid Cell Lines Cultivation Protocol
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Thyroid cancer cell lines (KAT5, Hth74, TPC-1 and IHH4) and human normal thyroid follicular epithelial cell line Nthy-ori3-1 were bought from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 100 mg/mL streptomycin, 100 U/mL penicillin and 10% fetal bovine serum (FBS; Gibco) in a humidified incubator containing 5% CO 2 at 37°C.
9
Culturing Human PTC Cell Lines
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Three human PTC cell lines (TPC-1, K1 and IHH-4) and a human thyroid epithelial cell line Nthy-ori3-1 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Although K1 cells are contaminated with GLAG-66 (11 (link)), the resulting phenotypic and genotypic differences between K1 and GLAG-66 cells were considered unlikely to affect our study. The PTC cells were supplemented with Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS; both Gibco; Thermo Fisher Scientific Inc., Waltham, MA, USA). Cells were cultured at 37°C in humidified air with 5% CO2.
10
Cell Culture of Thyroid Cancer Lines
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Human PTC cell lines (TPC-1 and K-1) and human thyroid epithelial cell line Nthy-ori3-1 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All cells were maintained in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS (HyClone, Logan, UT, USA) and 100 U/mL penicillin/ streptomycin (Sigma-Aldrich, St Louis, MO, USA) at 37°C in a humidified incubator with 5% CO2.
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