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The SNU-719 is a laboratory equipment used for cell culture applications. It provides a controlled environment for the growth and maintenance of cell lines. The core function of the SNU-719 is to maintain the optimal temperature, humidity, and gas composition required for the culture of cells.

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Lab products found in correlation

Hgc 27, by National Collection of Authenticated Cell Cultures (2 mentions) Sgc 7901, by National Collection of Authenticated Cell Cultures (1 mentions) Hek293, by National Collection of Authenticated Cell Cultures (1 mentions) Bgc 803, by National Collection of Authenticated Cell Cultures (1 mentions) Rpmi 1640 medium, by Wisent (1 mentions) Mkn 45, by National Collection of Authenticated Cell Cultures (1 mentions) Snu 1, by National Collection of Authenticated Cell Cultures (1 mentions) Mkn28, by National Collection of Authenticated Cell Cultures (1 mentions)

2 protocols using snu 719

1

Cell Line Cultivation Protocols

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The cell lines SGC-7901, BGC-803, HGC-27, AGS, SNU-719, and HEK293, as well as NK-92, were acquired from the Cell Bank of the Chinese Academy of Sciences in Shanghai, China. The cells were grown in medium with 10% fetal bovine serum (FBS), which was maintained at 37 °C with 5% CO2.
Chen X., Mao D., Li D., Li W., Wei H., Deng C., Chen H, & Zhang C. (2023). Identification and validation of a PD-L1-related signature from mass spectrometry in gastric cancer. Journal of Cancer Research and Clinical Oncology, 149(9), 5871-5884.
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2

Establishment of Stable Gastric Cancer-Associated Fibroblasts

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The human gastric mucosal epithelial cell GES-1 and GC cell lines SNU-1, HGC27, SNU-719, MKN28, MKN-45 and AGS were purchased from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). They were cultured in the condition of 5% CO2 and 37 °C, with RPMI-1640 medium (Wisent, 350-000-CL) contained 10% FBS. Primary GC cells, CAFs and NFs were cultured in DMEM contained 10% FBS.
In order to establish stable CAFs that knockdown SULF1, shRNA (GenePharma, China) sequences against SULF1 (shSULF1#1 and shSULF1#2) were packaged in lentivirus vectors. Sequences used for RNAi were listed in supplementary Table S1.
Fang X., Chen D., Yang X., Cao X., Cheng Q., Liu K., Xu P., Wang Y., Xu J., Zhao S, & Yan Z. (2024). Cancer associated fibroblasts-derived SULF1 promotes gastric cancer metastasis and CDDP resistance through the TGFBR3-mediated TGF-β signaling pathway. Cell Death Discovery, 10, 111.
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