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Bfgf fgf2

Manufactured by ReproCELL
Sourced in Japan

BFGF (FGF2) is a growth factor protein that plays a crucial role in various cellular processes, including cell proliferation, differentiation, and survival. It is a recombinant human fibroblast growth factor 2 (FGF2) produced in E. coli. BFGF (FGF2) is a versatile tool used in cell culture and tissue engineering applications.

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2 protocols using bfgf fgf2

1

Hepatic Maturation of hiPSC-Derived Endoderm

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hiPSC-derived endoderm was further treated with 1% Penicillin-Streptomycin Solution (×100) (16823191: FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 20 ng/mL Recombinant Human HGF Protein (294-HG-005: R&D SYSTEMS, Minneapolis, MN), 20 ng/mL Bone Morphogenetic Protein 4 (truncated) (BMP-4) (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 10 ng/mL bFGF (FGF2) (ReproCELL Inc., Kanagawa, Japan) in RPMI 1640 with L-Gln, liquid medium (30264–85: Nacalai Tesque, Kyoto, Japan), supplemented with 2% KSR (10828028: ThermoFisher Scientific, Tokyo, Japan) for 5 days in initial stage of hepatic maturation.
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2

Hepatocyte Induction from hiPSC-Derived Endoderm

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hiPSC-derived endoderm was further treated with 1% Penicillin-Streptomycin Solution (×100) (16823191: FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 20 ng/mL Bone Morphogenetic Protein 4 (truncated) (BMP-4) (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 200 ng/mL Recombinant Human Sonic Hedgehog/Shh Protein, High Activity (8908-SH-005: R&D SYSTEMS, Minneapolis, MN), 10 ng/mL bFGF (FGF2) (ReproCELL Inc., Kanagawa, Japan) in RPMI 1640 with L-Gln, liquid medium (30264–85: Nacalai Tesque, Kyoto, Japan), supplemented with 2% KSR (10828028: ThermoFisher Scientific, Tokyo, Japan) for 5 days for the induction of hepatocytes.
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