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Rabbit anti ccdc170

Manufactured by GeneTex
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Rabbit anti-CCDC170 is a primary antibody that recognizes the Coiled-Coil Domain Containing 170 protein. It is produced in rabbit and can be used for various research applications, such as Western blotting, immunohistochemistry, and immunofluorescence.

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Lab products found in correlation

17β estradiol e2, by Merck Group (2 mentions) Cyclin d1, by Thermo Fisher Scientific (2 mentions) Mouse anti cmet and rabbit anti gab1, by Cell Signaling Technology (2 mentions) 4 oh tamoxifen tam, by Merck Group (2 mentions)

2 protocols using rabbit anti ccdc170

1

Protein Detection in Serum-Depleted Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein samples were separated in SDS-PAGE gel and transferred either onto 0.2μm PVDF membrane for detection of the E2-E10 protein product, or 0.2μm nitrocellulose membrane for other proteins. The dilutions of primary antibodies used were 1:250-1:1000 for rabbit anti-CCDC170 (GeneTex), 1:1000 for mouse anti-cMet and rabbit anti-Gab1 (Cell Signaling) and other antibodies. Antibodies were obtained from Santa Cruz (cyclin D1), Thermo Fisher (ERα), Abcam (PAK1), Millipore (Src), and Cell Signaling (all other molecules). To study the fusion-driven signaling in the condition of serum withdrawal and endocrine treatment, cells were maintained in phenol red-free medium for 48h, serum-starved for 24h, and then treated for 20 minutes with vehicle (Ethanol), 17β-estradiol (E2) (1nM) or Tam (100nM). 4-OH tamoxifen (Tam) and 17β-estradiol (E2) were obtained from Sigma-Aldrich.
Veeraraghavan J., Tan Y., Cao X.X., Kim J.A., Wang X., Chamness G.C., Maiti S.N., Cooper L.J., Edwards D.P., Contreras A., Hilsenbeck S.G., Chang E.C., Schiff R, & Wang X.S. (2014). Recurrent ESR1-CCDC170 rearrangements in an aggressive subset of estrogen-receptor positive breast cancers. Nature communications, 5, 4577.
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2

Protein Detection in Serum-Depleted Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein samples were separated in SDS-PAGE gel and transferred either onto 0.2μm PVDF membrane for detection of the E2-E10 protein product, or 0.2μm nitrocellulose membrane for other proteins. The dilutions of primary antibodies used were 1:250-1:1000 for rabbit anti-CCDC170 (GeneTex), 1:1000 for mouse anti-cMet and rabbit anti-Gab1 (Cell Signaling) and other antibodies. Antibodies were obtained from Santa Cruz (cyclin D1), Thermo Fisher (ERα), Abcam (PAK1), Millipore (Src), and Cell Signaling (all other molecules). To study the fusion-driven signaling in the condition of serum withdrawal and endocrine treatment, cells were maintained in phenol red-free medium for 48h, serum-starved for 24h, and then treated for 20 minutes with vehicle (Ethanol), 17β-estradiol (E2) (1nM) or Tam (100nM). 4-OH tamoxifen (Tam) and 17β-estradiol (E2) were obtained from Sigma-Aldrich.
Veeraraghavan J., Tan Y., Cao X.X., Kim J.A., Wang X., Chamness G.C., Maiti S.N., Cooper L.J., Edwards D.P., Contreras A., Hilsenbeck S.G., Chang E.C., Schiff R, & Wang X.S. (2014). Recurrent ESR1-CCDC170 rearrangements in an aggressive subset of estrogen-receptor positive breast cancers. Nature communications, 5, 4577.
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