Antibodies used for immunoblotting: rabbit anti-ErbB2 (29D8, Cell Signaling), mouse anti-ErbB2 (Ab-17, Thermo Fisher Scientific), rabbit anti-ErbB3 (Ab-1328, Sigma Aldrich, MO), rabbit anti-Ebp50 (SLC9A3R1, Sigma Aldrich), rabbit anti-Ebp50 (A310, Cell Signaling), mouse anti-ezrin (E8897, Sigma Aldrich), rabbit anti-radixin (HPA000763, Sigma Aldrich), rabbit anti-pERM (3149, Cell Signaling), mouse anti-GAPDH (Ab-9484, Abcam), mouse anti-pErk1/2 (9106, Cell Signaling), rabbit anti-pAkt (4058, Cell Signaling), mouse anti-Ubiquitin (P4G7, Covance). Antibodies used for microscopical analysis: rabbit anti-ErbB2 (29D8, Cell Signaling), mouse anti-ErbB2 (9G6, Santa Cruz), rabbit anti-pERM (3149, Cell Signaling), rabbit anti-ezrin (3145, Cell Signaling), mouse anti-ezrin (E8897, Sigma Aldrich), rabbit anti-radixin (HPA000763, Sigma Aldrich), mouse anti-radixin (1E12, Novus Biologicals), rabbit anti-Ebp50 (SLC9A3R1, Sigma Aldrich), mouse anti-Hsp90 (sc-13119, Santa Cruz), mouse anti-c-Cbl (Upstate). HEPES, bovine serum albumin, geldanamycin, lactacystin, chloroquine and n-octylglucopyranoside were purchased from Sigma-Aldrich. The small molecule inhibitor NSC668394 was purchased from Calbiochem, and the dynamin-inhibitor Dyngo4a was purchased from Fisher Scientific. The plasmids encoding GFP-ErbB2 and CFP-ErbB3 were kind gifts from Dr. B. van Deurs (University of Copenhagen, Denmark).
Asp N., Kvalvaag A., Sandvig K, & Pust S. (2016). Regulation of ErbB2 localization and function in breast cancer cells by ERM proteins. Oncotarget, 7(18), 25443-25460.
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