IS potassium salt and isotope‐labeled IS potassium salt (13C6, 99%) as internal standard were purchased from Sigma Aldrich (Zwijndrecht, The Netherlands) and Cambridge Isotope Laboratory (Tewksbury, Massachusetts, USA) (Merck, Schiphol‐Rijk, The Netherlands), respectively. Water (ULC–MS grade), acetonitrile (ACN) (HPLC‐S grade), and methanol (HPLC grade) were purchased from Biosolve (Valkenswaard, The Netherlands). Formic acid (analytical grade) was obtained from Merck (Darmstadt, Germany). Ultrapure water was produced by a Milli‐Q® Advantage A10 Water Purification Systems (Merck, The Netherlands). Bovine serum albumin (BSA) was obtained from Roche (Mannheim, Germany).
Methanol hplc grade
Manufactured by Biosolve
Sourced in Netherlands, France
Methanol (HPLC grade) is a high-purity solvent used in various analytical and chromatographic applications, particularly in high-performance liquid chromatography (HPLC). It is a colorless, volatile, and flammable liquid with a low boiling point.
Automatically generated - may contain errors
Lab products found in correlation
Milli q advantage a10 water purification system, by Merck Group (3 mentions)
Formic acid, by Merck Group (3 mentions)
Is potassium salt, by Merck Group (2 mentions)
Acetonitrile, by Biosolve (2 mentions)
Bovine serum albumin (bsa), by Roche (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Red phosphorus, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Merck Group (1 mentions)
Acetone, by Biosolve (1 mentions)
Bms345541, by Axon Medchem (1 mentions)
Cell culture plates, by Greiner (1 mentions)
Hydrochloric acid solution, by Merck Group (1 mentions)
Millipore milli q system, by Merck Group (1 mentions)
Lactose, by Merck Group (1 mentions)
Ammonia solution, by Merck Group (1 mentions)
Ethanol, by Biosolve (1 mentions)
Hplc grade acetonitrile, by Biosolve (1 mentions)
Milli q unit, by Merck Group (1 mentions)
Stevioside, by ChromaDex (1 mentions)
Milli q water 18.2 mω cm, by Merck Group (1 mentions)
14 protocols using methanol hplc grade
1
Quantification of Potassium Isotopes
2
Quantification of Potassium Isotopes
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IS potassium salt and isotope-labeled IS potassium salt (13C6, 99%) as internal standard were purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands) and Cambridge Isotope Laboratory (Tewksbury, Massachusetts, USA), respectively. Water (U°LC-MS grade), acetonitrile (HPLC-S grade), and methanol (HPLC grade) were purchased from Biosolve (Valkenswaard, The Netherlands). Formic acid (analytical grade) was obtained from Merck (Darmstadt, Germany). Ultrapure water was produced by a Milli-Q® Advantage A10 Water Purification Systems (Merck, Amsterdam, The Netherlands).
3
Quantification of Pazopanib in Plasma
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Pazopanib (as hydrochloride) and stable isotopically labeled internal standard (IS) 13 C, 2 H 3 -pazopanib, with purities (as free base) of 92.3% and 92.6% were supplied by GlaxoSmithKline (Zeist, The Netherlands). Dimethyl sulfoxide (DMSO) was purchased from Merck (Darmstadt, Germany), ammonia (Empure 25%), and methanol (HPLC grade) from BioSolve Ltd (Valkenswaard, The Netherlands). Control human EDTA-plasma was obtained from healthy volunteers and used for the preparation of quality control (QC) samples, calibrators, and matrix blanks.
4
Mass Spectrometry Lipid Analysis
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Acetone and methanol (HPLC
grade) were obtained from Biosolve (Valkenswaard, Netherlands). Trifluoroacetic
acid (TFA, 99%), α-cyano-4-hydroxycinnamic acid (α-HCCA,
purity 97%), and red phosphorus (>97%) were purchased from Sigma-Aldrich
(Taufkirchen, Germany). The internal standard SPLASH LipidoMIX containing
deuterated lipids from different families was purchased from Avanti
Polar Lipids (Alabaster, Alabama) via Sigma-Aldrich.
grade) were obtained from Biosolve (Valkenswaard, Netherlands). Trifluoroacetic
acid (TFA, 99%), α-cyano-4-hydroxycinnamic acid (α-HCCA,
purity 97%), and red phosphorus (>97%) were purchased from Sigma-Aldrich
(Taufkirchen, Germany). The internal standard SPLASH LipidoMIX containing
deuterated lipids from different families was purchased from Avanti
Polar Lipids (Alabaster, Alabama) via Sigma-Aldrich.
5
Evaluation of Uremic Toxins on Inflammation
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All reagents were purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands) unless stated otherwise. In the present study, lipopolysaccharide (LPS) from Escherichia coli 0127:B8 was used. The toxins, p-cresyl glucuronide (pCG) and p-cresyl sulfate (pCS), were synthesized by the Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands, as previously described [36 (link)]. IκB kinase (IKK) inhibitor BMS-345541 was obtained from Axon Medchem (Groningen, The Netherlands). Water (LC-MS grade), acetonitrile (ACN; HPLC-S grade) and methanol (HPLC grade) were obtained from Biosolve (Valkenswaard, The Netherlands). Formic acid (analytical grade) was purchased at Merck (Darmstadt, Germany). Ultrapure water was produced by a Milli-Q® Advantage A10 Water Purification Systems (Merck, Schiphol-Rijk, The Netherlands). Cell culture plates were obtained from Greiner Bio-One (Monroe, NC, USA).
6
Characterization of Adulterated Botanical Supplements
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Reference material for Aristolochia fanghi, Ilex paraguariensis, Hoodia gordonii, and Garcinia cambogia was obtained from the American Herbal Pharmacopoeia (Scotts Valley, CA, USA), which authenticated the plant material through different macro- and microscopic techniques and provided a validation certificate confirming its identity.
Reagents such as methanol (HPLC grade), acetonitrile (HPLC grade), and ethanol (96% v/v) were obtained from Biosolve (Valkenswaard, The Netherlands). Formic acid (99.7%), hydrochloric acid solution (37 w/w%), and ammonia solution (25 w/w%) were all purchased from Merck (Darmstadt, Germany). A Millipore—MilliQ® system (Billerica, MA, USA) was used to dispense milli-Q water. Lactose was also procured from Merck (Darmstadt, Germany).
All botanical supplements used for triturations and samples were selected from the samples seized by the Federal Agency for the Safety of the Food Chain (FASFC) (Brussels, Belgium) and sent to the laboratory to be tested for chemical adulteration. A choice for the botanical matrices to be used for triturations was made such that no concerned plant in this paper was listed on the label, and the supplement itself did not mention slimming as an indication. On the other hand, 12 samples were chosen, out of which 9 (samples 1–9) were slimming aids while 3 (samples 10–12) were potency enhancers.
Reagents such as methanol (HPLC grade), acetonitrile (HPLC grade), and ethanol (96% v/v) were obtained from Biosolve (Valkenswaard, The Netherlands). Formic acid (99.7%), hydrochloric acid solution (37 w/w%), and ammonia solution (25 w/w%) were all purchased from Merck (Darmstadt, Germany). A Millipore—MilliQ® system (Billerica, MA, USA) was used to dispense milli-Q water. Lactose was also procured from Merck (Darmstadt, Germany).
All botanical supplements used for triturations and samples were selected from the samples seized by the Federal Agency for the Safety of the Food Chain (FASFC) (Brussels, Belgium) and sent to the laboratory to be tested for chemical adulteration. A choice for the botanical matrices to be used for triturations was made such that no concerned plant in this paper was listed on the label, and the supplement itself did not mention slimming as an indication. On the other hand, 12 samples were chosen, out of which 9 (samples 1–9) were slimming aids while 3 (samples 10–12) were potency enhancers.
7
VAMS Microsampling of Miltefosine
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VAMS devices (brand name Mitra TM ) were purchased from Neoteryx, LLC (Torrance, CA, USA) (Phenomenex, Utrecht, the Netherlands). Miltefosine (Fig. 2) was supplied by Sigma-Aldrich (Zwijndrecht, the Netherlands) and deuterated miltefosine (miltefosine-D4, Fig. 2) was purchased from Alsachim (Illkirch Graffenstaden, France). Methanol (HPLC grade) and water (HPLC grade) were purchased from Biosolve Ltd. (Valkenswaard, the Netherlands). Ammonia (25%) was purchased from Merck (Amsterdam, the Netherlands).
Whole blood was collected from healthy volunteers in K 2 EDTA BD Vacutainers ® and adjusted to mimic typical Hct values of VL patients (between 23 and 37% [6] (link)) by addition of blood plasma of the same volunteer obtained after whole blood centrifugation. Hct levels were determined with either the Cell Dyn Hematology analyser (Abbot Diagnostics, Lake Forest, IL, USA) or the XN-3000 Hematology analyser (Sysmex, Kobe, Japan). Whole blood was stored at 2-8 • C no longer than one week before the preparation of validation samples.
Whole blood was collected from healthy volunteers in K 2 EDTA BD Vacutainers ® and adjusted to mimic typical Hct values of VL patients (between 23 and 37% [6] (link)) by addition of blood plasma of the same volunteer obtained after whole blood centrifugation. Hct levels were determined with either the Cell Dyn Hematology analyser (Abbot Diagnostics, Lake Forest, IL, USA) or the XN-3000 Hematology analyser (Sysmex, Kobe, Japan). Whole blood was stored at 2-8 • C no longer than one week before the preparation of validation samples.
8
Sweetener Profiling in Diverse Beverages
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Food samples were purchased from the local markets for food safety inspection by the Public Health Bureau, Pingtung County Government, Pingtung, China from January 2012 to May 2012. The samples included 11 nonalcoholic beverages (i.e., 1 lemon tea, 2 sports drink, 2 soft drinks, and 6 juices), 16 alcoholic beverages (i.e., 1 cocktail, 3 plum flavored wine, 8 grape wines, and 4 flavored beers), and 15 preserved fruits and vegetables (i.e., 1 pickled pepper, 11 preserved fruits, and 3 candies).
ASP (99.9%), sodium CYC (99.9%), and sodium SAC (99.9%) were purchased from Supelco Co. (Bellefonte, PA, USA), whereas ACS-K (99%) and SCL (98%) were obtained from Fluka Chemie (Buchs, Switzerland). Stevioside (STV; 98.4%) and GA (98.9%) were purchased from ChromaDex Inc. (Irvine, CA, USA). NEO (99%) and NHDC (99.9%) were obtained from Sigma-Aldrich (Munich, Germany). DUL (99.8%) was obtained from Kanto Chemical Co., Inc. (Tokyo, Japan). All chemicals were of analytical grade. Methanol (HPLC-grade) was obtained from Biosolve (Valkenswaard, The Netherlands), whereas purified deionized water (R = 18 MΩ cm) was produced by a MilliQ unit (Millipore, Billerica, MA, USA). The chemical structures of all 10 sweeteners are shown inFig. 1 . Formic acid and acetonitrile were of HPLC grade and purchased from J.T. Baker Co., Ltd. (Philipsburg, NJ, USA). All other reagents were of analytical grade.
ASP (99.9%), sodium CYC (99.9%), and sodium SAC (99.9%) were purchased from Supelco Co. (Bellefonte, PA, USA), whereas ACS-K (99%) and SCL (98%) were obtained from Fluka Chemie (Buchs, Switzerland). Stevioside (STV; 98.4%) and GA (98.9%) were purchased from ChromaDex Inc. (Irvine, CA, USA). NEO (99%) and NHDC (99.9%) were obtained from Sigma-Aldrich (Munich, Germany). DUL (99.8%) was obtained from Kanto Chemical Co., Inc. (Tokyo, Japan). All chemicals were of analytical grade. Methanol (HPLC-grade) was obtained from Biosolve (Valkenswaard, The Netherlands), whereas purified deionized water (R = 18 MΩ cm) was produced by a MilliQ unit (Millipore, Billerica, MA, USA). The chemical structures of all 10 sweeteners are shown in
9
Quantification of Ponatinib and Metabolite
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Ponatinib (>98%) was supplied by Sequoia Research Products (Pangbourne, United Kingdom), DMP (97.0%), ponatinib-d 8 (99.5%, isotopic purity >99%, Fig. 1C) and DMP-d 8 (96.8%, isotopic purity >99%, Fig. 1D) were obtained from Alsachim (Illkirch Graffenstaden, France). Water (LC-MS grade) and methanol (HPLC grade) were obtained from Biosolve (Valkenswaard, The Netherlands). Water not used as eluent was home purified by reversed osmosis on a multi-laboratory scale. Ammonium hydroxide (ACS reagent grade) originated from Sigma-Aldrich (Steinheim, Germany) and tert-butyl methyl ether (tBME, Lichrosolve quality) from Merck (Darmstadt, Germany). Pooled mouse lithium heparin plasma was supplied by Seralab Laboratories International (Haywards Heath, UK).
10
LCXLC-MS Analyte Profiling Protocol
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Acetonitrile (ACN, MS grade), methanol (HPLC grade) and water (MS grade) were purchased from Biosolve Chimie (Dieuze, France). Milli-Q water (18.2 MΩ cm) was obtained from a purification system (Millipore, Bedford, MA, USA). Ammonium acetate (AcNH4, analytical grade; 98%) and acetic acid (analytical grade; 99%) were purchased from Sigma-Aldrich (Darmstadt, Germany). The "Perfluorinated Native Compound Standard" mixture (PFC-24-10X) adopted as the analyte training set for method development, was purchased by AccuStandard (New Haven, CT, USA), and its details and composition are reported in The equipment used for the modulation valve of the LC × LC system consisted of a capillary (0.12 × 120 mm, Agilent Technologies, Waldbronn, Germany) and two C18 guard columns (SecurityGuard Ultra AJ0-87822.1 × 2 mm and sub-2 μm particle, Phenomenex, Utrecht, NL) used for analyte trapping.
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