Hplc grade dichloromethane dcm

Poly(L-lactide-co-ɛ-caprolactone) (PLC, molar ratio 70/30, Mw = 202,000 g/mol) was purchased from Corbion Purac, The Netherlands. Levofloxacin and Moxifloxacin were purchased from Molekula Limited, UK. Phosphate buffer saline tablets (PBS) were purchased from Sigma-Aldrich, Singapore. HPLC grade dichloromethane (DCM) and tetrahydrofuran (THF) were obtained from Tedia, USA.

PLGA 50/50, indicating a lactic/glycolic acid composition of 50%/50% (mol), consists of two different IV: 1.18 dL g⁻¹ from Bioinvigor and 1.01 dL g⁻¹ from Purac.
Two types of poly(vinyl alcohol) (PVA) were used to create the particles: PVA-403 was purchased from Kuraray, with a hydrolysis rate of 78% − 82% and MW ∼ 15 000 Da according to the manufacturer’s specification, and PVA 30000 was purchased from Sigma-Aldrich, with a hydrolysis rate of 87% − 90% and MW of 30000 − 70000 Da.
Bovine serum albumin (BSA) and the polyelectrolytes used: PAH (MW ∼ 56000 Da), PSS (MW ∼ 70000 Da), PLL hydrobromide (MW 30000 − 70000 Da), and DES (MW 36000 − 50000 Da), were purchased from Sigma-Aldrich. HPLC-grade dichloromethane (DCM) and dimethyl sulfoxide (DMSO) were purchased from Tedia, with Millipore water being used for all the experiments. All salts for buffer preparations—phosphate buffer saline (PBS) and carbonate buffer—were of analytical grade, purchased from Sigma, except NaCl and KCl, from VWR, and Na₂HPO₄, from Alfa Aesar.