Log In Sign up
The largest database of trusted experimental protocols

Jai mmd 030n

Manufactured by Adipogen
Visit Supplier

The JAI-MMD-030N is a high-resolution camera system designed for industrial, scientific, and medical applications. It features a compact and robust design, and it is capable of capturing images with a resolution of up to 3.2 megapixels. The camera is equipped with a global shutter sensor and can operate at frame rates of up to 60 frames per second.

Automatically generated - may contain errors

Lab products found in correlation

Dab peroxidase substrate kit, by Vector Laboratories (2 mentions) Vectastain elite abc kit, by Vector Laboratories (2 mentions) Bx43 microscope, by Olympus (2 mentions) Ab46545, by Abcam (2 mentions) G1101, by Wuhan Servicebio Technology (1 mentions)

3 protocols using jai mmd 030n

1

Histological Analysis of Kidney Damage

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fresh kidney tissues were fixed in 10% neutral buffered formalin overnight, washed once with PBS, and stored in 70% ethanol at 4°C. The tissues were dehydrated and embedded in paraffin by Research Histology Core Laboratory (MD Anderson Cancer Center) according to standard protocols. Embedded tissues were sectioned at a thickness of 5 μm for hematoxylin & eosin (H&E), 4-HNE and MDA staining. H&E stained kidney sections were analyzed using an Olympus BX43 microscope. Organ damage on the renal cortex was evaluated by the following parameters: tubular dilatation, tubule blush border loss, flattened epithelial cells, and sloughing of cells. The primary antibodies, 4-HNE (ab46545, Abcam, 1:200) or MDA (JAI-MMD-030N, Adipogen, 1:100), were incubated overnight at 4°C. Staining was performed using the Vectastain elite ABC kit and DAB peroxidase substrate kit (Vector laboratories). Images were randomly taken from the renal cortex (10 images per mouse) at 200 X magnification using an Olympus BX43 microscope, and the percentage of 4-HNE positive tubular cells per image was analyzed.
Lee H., Zandkarimi F., Zhang Y., Meena J.K., Kim J., Zhuang L., Tyagi S., Ma L., Westbrook T.F., Steinberg G.R., Nakada D., Stockwell B.R, & Gan B. (2020). Energy stress-mediated AMPK activation inhibits ferroptosis. Nature cell biology, 22(2), 225-234.
+ Open protocol
+ Expand
2

Immunostaining Xenograft Tissue Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Xenograft tissue samples were collected and immediately fixed in neutral paraformaldehyde fixing solution (G1101; Servicebio) overnight and then submitted to the Servicebio company for embedding and hematoxylin and eosin staining. For IF and immunohistochemical (IHC) staining, tissue sections were processed according to the instructions of Servicebio. Briefly, tissue sections were deparaffinized, rehydrated, and incubated with the corresponding primary and secondary antibodies after antigen repair. The cell nuclei of the tissue sections were restained with DAPI, sealed with blocker, and then photographed by microscopy. The antibodies used for IF were anti-SLC7A11 and anti-ZRANB1, and the antibody used for IHC was anti-MDA (JAI-MMD-030 N; Adipogen, 1:100, Mouse IgG).
Huang S., Zhang Q., Zhao M., Wang X., Zhang Y., Gan B, & Zhang P. (2023). The deubiquitinase ZRANB1 is an E3 ubiquitin ligase for SLC7A11 and regulates ferroptotic resistance. The Journal of Cell Biology, 222(11), e202212072.
+ Open protocol
+ Expand
3

Histological Analysis of Kidney Damage

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fresh kidney tissues were fixed in 10% neutral buffered formalin overnight, washed once with PBS, and stored in 70% ethanol at 4°C. The tissues were dehydrated and embedded in paraffin by Research Histology Core Laboratory (MD Anderson Cancer Center) according to standard protocols. Embedded tissues were sectioned at a thickness of 5 μm for hematoxylin & eosin (H&E), 4-HNE and MDA staining. H&E stained kidney sections were analyzed using an Olympus BX43 microscope. Organ damage on the renal cortex was evaluated by the following parameters: tubular dilatation, tubule blush border loss, flattened epithelial cells, and sloughing of cells. The primary antibodies, 4-HNE (ab46545, Abcam, 1:200) or MDA (JAI-MMD-030N, Adipogen, 1:100), were incubated overnight at 4°C. Staining was performed using the Vectastain elite ABC kit and DAB peroxidase substrate kit (Vector laboratories). Images were randomly taken from the renal cortex (10 images per mouse) at 200 X magnification using an Olympus BX43 microscope, and the percentage of 4-HNE positive tubular cells per image was analyzed.
Lee H., Zandkarimi F., Zhang Y., Meena J.K., Kim J., Zhuang L., Tyagi S., Ma L., Westbrook T.F., Steinberg G.R., Nakada D., Stockwell B.R, & Gan B. (2020). Energy stress-mediated AMPK activation inhibits ferroptosis. Nature cell biology, 22(2), 225-234.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!