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Safefix

Manufactured by Thermo Fisher Scientific
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SafeFix is a laboratory equipment product designed for fixing and preserving biological samples. It provides a reliable and consistent method for sample preparation and preservation, ensuring the integrity of specimens for further analysis.

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Lab products found in correlation

Anti goat hrp dab cell tissue staining kit, by R&D Systems (1 mentions) Histostain plus kit, by Thermo Fisher Scientific (1 mentions) Alexa 594, by Jackson ImmunoResearch (1 mentions) Alexa 488, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

SafeFix II (19 citations) SafeFix solution (6 citations)

5 protocols using safefix

1

Histological Scoring of Pericarditis

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Hearts were cut transversely, fixed in SafeFix (Thermo Fisher Scientific), embedded in paraffin, cut into 5 μm-thick sections and stained with H&E (Histoserv, MD). The severity of pericarditis was assessed by scoring infiltration of the area of pericardium around right ventricle (RV) on H&E-stained sections based on histopathology score from 0 to 4 using the following criteria for hematopoietic infiltrates: grade 0, no inflammation; grade 1, < 20% of RV is involved and/or mild inflammation; grade 2, 20%–50% of RV is involved and/or intermediate inflammation; grade 3, 50%–80% of RV is involved and/or severe inflammation; grade 4, >80% of RV is involved and/or severe inflammation with adjacent myocardial infiltrates. Scoring was performed by two blinded investigators and averaged.
Choi H.S., Won T., Hou X., Chen G., Bracamonte-Baran W., Talor M.V., Jurčová I., Szárszoi O., Čurnova L., Stříž I., Hooper J.E., Melenovský V, & Čiháková D. (2020). Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis. Cell reports, 30(9), 2989-3003.e6.
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2

Histological Evaluation of Myocarditis

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Mice were most commonly evaluated for the development of EAM at the peak of disease on day 21. Heart tissues were fixed in SafeFix (Thermo Fisher Scientific). Tissues were embedded longitudinally, and 5 μm serial sections were cut and stained with hematoxylin and eosin, or Masson’s Trichrome Blue (HistoServ). Myocarditis severity was evaluated by microscopic estimation of the percent area of myocardium infiltrated with inflammatory cells, fibrosis, and cardiomyocyte necrosis determined from five sections per heart according to the following scoring system: grade 0 – no inflammation; grade 1 – < 10% of the heart section is involved; grade 2 – 10–30%; grade 3 – 30–50%; grade 4 – 50–90%; grade 5 – > 90%. Grading was performed by a minimum of two independent blinded investigators and averaged (Smith, 2005 ).
Barin J.G., Talor M.V., Diny N.L., Ong S., Schaub J.A., Gebremariam E., Bedja D., Chen G., Choi H.S., Hou X., Wu L., Cardamone A.B., Peterson D.A., Rose N.R, & Čiháková D. (2017). Regulation of autoimmune myocarditis by host responses to the microbiome. Experimental and molecular pathology, 103(2), 141-152.
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3

Quantifying Eosinophil Infiltration in Mouse Hearts

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Mouse hearts were cut in half, fixed in SafeFix (Thermo Fisher Scientific), paraffin embedded, and cut into 5-μm-thick sections (Histoserv). Serial sections were stained with 5 μg/mL polyclonal goat anti-mouse CCL11 or CCL24 antibodies (R&D Systems) using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (R&D Systems) and counterstained with Hematoxylin. The number of infiltrating eosinophils was determined by counting eosinophils on 40×images of H&E-stained biopsy sections. The number of eosinophils was averaged over the area observed.
Diny N.L., Hou X., Barin J.G., Chen G., Talor M.V., Schaub J., Russell S.D., Klingel K., Rose N.R, & Čiháková D. (2016). Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart. European journal of immunology, 46(12), 2749-2760.
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4

Histological Scoring of Pericarditis

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Hearts were cut transversely, fixed in SafeFix (Thermo Fisher Scientific), embedded in paraffin, cut into 5 μm-thick sections and stained with H&E (Histoserv, MD). The severity of pericarditis was assessed by scoring infiltration of the area of pericardium around right ventricle (RV) on H&E-stained sections based on histopathology score from 0 to 4 using the following criteria for hematopoietic infiltrates: grade 0, no inflammation; grade 1, < 20% of RV is involved and/or mild inflammation; grade 2, 20%–50% of RV is involved and/or intermediate inflammation; grade 3, 50%–80% of RV is involved and/or severe inflammation; grade 4, >80% of RV is involved and/or severe inflammation with adjacent myocardial infiltrates. Scoring was performed by two blinded investigators and averaged.
Choi H.S., Won T., Hou X., Chen G., Bracamonte-Baran W., Talor M.V., Jurčová I., Szárszoi O., Čurnova L., Stříž I., Hooper J.E., Melenovský V, & Čiháková D. (2020). Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis. Cell reports, 30(9), 2989-3003.e6.
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5

Immunohistochemistry and Immunofluorescence Protocols

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For paraffin sections, tissues were fixed in Safefix (Thermo Fisher Scientific, Lafayette, CO, USA) and embedded in paraffin. After deparaffinization and hydration, sections (6 μm) were subjected to antigen retrieval by autoclaving in 0.01M sodium citrate solution (pH = 6) for 10 min. For frozen tissues, sections (12 μm) were fixed in 4% paraformaldehyde solution. Depending on the primary antibody (S1 Table), some sections were subjected to antigen retrieval by autoclaving in 0.01M sodium citrate solution (pH = 6) for 10 min. COX-2 and TGFβ antibodies were custom-made as previously described [54 (link), 55 (link)]. For immunohistochemistry, Histostain-Plus kit (Invitrogen, Carlsbad, CA, USA) was used to visualize signals. Immunofluorescence was performed using secondary antibodies conjugated with Alexa 488 or Alexa 594 (Jackson ImmunoResearch, West Grove, PA, USA). Hematoxylin and Hoechst were used for counterstain in immunohistochemistry and immunofluorescence, respectively. For all images of pHH3 staining at lower magnification, the maximum filter of ImageJ was applied to the red staining channel for clear visibility.
Liang X., Daikoku T., Terakawa J., Ogawa Y., Joshi A.R., Ellenson L.H., Sun X, & Dey S.K. (2018). The uterine epithelial loss of Pten is inefficient to induce endometrial cancer with intact stromal Pten. PLoS Genetics, 14(8), e1007630.
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