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70um strainers

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70μm strainers are laboratory filtration devices used to separate particles or cells larger than 70 micrometers from a liquid or suspension. They provide a consistent pore size to enable efficient filtration.

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Lab products found in correlation

Ckit a4502, by Agilent Technologies (2 mentions) Facsaria 2 cell sorter, by BD (2 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

70um Nylon cell strainer (3 citations)

3 protocols using 70um strainers

1

Single-Cell Isolation and Flow Cytometry

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Cells were dissociated in 0.25% trypsin--EDTA (Gibco BRL) at 37°C for 5 min and collected by centrifugation at 200g in an Eppendorf 5702 R centrifuge. Then the cells were passed through the 70uM strainers (BD Biosciences) to make sure they were digested as single cells before they were subject to the flow cytometry. Mouse testes cells were dissociated with 0.25% Trypsin–EDTA for 30 minutes at 37 °C. Dissociated cells were incubated in 1% BSA in PBS containing primary antibodies (CKIT (A4502; DAKO)) on ice for 20 minutes. Cells were then analyzed for mOrange expression or CKIT/GFP using the BD FACSAriaII cell sorter. Analysis was performed using LSRII (Becton Dickinson) and FlowJo software (Tree Star).
Fang F., Angulo B., Xia N., Sukhwani M., Wang Z., Carey C.C., Mazurie A., Cui J., Wilkinson R., Wiedenheft B., Irie N., Surani M.A., Orwig K.E, & Pera R.A. (2018). A PAX5-OCT4-PRDM1 Developmental Switch Specifies Human Primordial Germ Cells. Nature cell biology, 20(6), 655-665.
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2

Fixation, Dissociation, and Flow Cytometry

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Cells may be fixed with 4% paraformaldehyde and stained with DAPI at room temperature for 15 min. Then, cells were dissociated in 0.25% trypsin--EDTA (Gibco BRL) at 37 °C for 5 min and collected by centrifugation at 200×g in an Eppendorf 5702R centrifuge. Then the cells were passed through the 70uM strainers (BD Biosciences) to make sure they were digested as single cells before they were subject to the flow cytometry. The analysis was performed using LSRII (Becton Dickinson) and FlowJo software (Tree Star).
Fang F., Xia N., Angulo B., Carey J., Cady Z., Durruthy-Durruthy J., Bennett T., Sebastiano V, & Reijo Pera R.A. (2018). A distinct isoform of ZNF207 controls self-renewal and pluripotency of human embryonic stem cells. Nature Communications, 9, 4384.
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3

Single-Cell Isolation and Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were dissociated in 0.25% trypsin--EDTA (Gibco BRL) at 37°C for 5 min and collected by centrifugation at 200g in an Eppendorf 5702 R centrifuge. Then the cells were passed through the 70uM strainers (BD Biosciences) to make sure they were digested as single cells before they were subject to the flow cytometry. Mouse testes cells were dissociated with 0.25% Trypsin–EDTA for 30 minutes at 37 °C. Dissociated cells were incubated in 1% BSA in PBS containing primary antibodies (CKIT (A4502; DAKO)) on ice for 20 minutes. Cells were then analyzed for mOrange expression or CKIT/GFP using the BD FACSAriaII cell sorter. Analysis was performed using LSRII (Becton Dickinson) and FlowJo software (Tree Star).
Fang F., Angulo B., Xia N., Sukhwani M., Wang Z., Carey C.C., Mazurie A., Cui J., Wilkinson R., Wiedenheft B., Irie N., Surani M.A., Orwig K.E, & Pera R.A. (2018). A PAX5-OCT4-PRDM1 Developmental Switch Specifies Human Primordial Germ Cells. Nature cell biology, 20(6), 655-665.
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