Diamonsil c18 2 column

HPLC conditions were as follows. Column: Diamonsil C18(2) column (250×4.6 mm, 5 μm) from Dikma Technologies Inc. (Beijing, People’s Republic of China); column temperature: 30°C; mobile phase: acetonitrile and phosphate buffer solution pH 3.0 (9:91, v/v) were used as the mobile phase; flow rate: 1.0 mL·min⁻¹; detection wavelength: 225 nm. Every sample was filtered through a 0.22 μm membrane filter before injection into the HPLC system. Under those conditions, the retention time of febrifugine was about 15.8 minutes. The assay was linear (r²=0.9999) in the concentration range 0.061–15.6 μg·mL⁻¹. This method was validated for linearity, precision, repeatability, stability, and accuracy, and the relative standard deviation was less than 3.5% in all cases. The methods of HPLC analysis were according to a previous study.20

HPLC conditions were as follows. Column: Diamonsil C18(2) column (250×4.6 mm, 5 μm) from Dikma Technologies Inc. (Beijing, People’s Republic of China); column temperature: 30°C; mobile phase: acetonitrile and phosphate buffer solution pH 3.0 (40:60, v/v) were used as the mobile phase; flow rate: 1.0 mL·min⁻¹; detection wavelength: 210 nm. Every sample was filtered through a 0.22 μm membrane filter before injection into the HPLC system. Under those conditions, the retention time of artesunate was approximately 36.5 minutes. The assay was linear (r²=0.9999) in the concentration range 2.05–205 μg·mL⁻¹. This method was validated for linearity, precision, repeatability, stability, and accuracy, and the relative standard deviation was less than 2.0% in all cases. The methods of HPLC analysis were according to the Pharmacopoeia of the People’s Republic of China 2010 and a previous study.18 ,19