Western blotting was performed as described previously (Chen et al. 2013b (link); Sun et al. 2014 (link)). Proteins were probed with the following antibodies: SIAH1 rabbit pAb (Abcam, Cambridge, MA, USA), Phospho-p53 (Ser15) rabbit pAb (Cell Signaling, Beverly, MA, USA), p53 mouse mAb (Abcam, Cambridge, MA, USA), β-Actin mouse mAb (Santa Cruz, SantaCruz, CA, USA), Phospho-p38 MAPK (Thr180/Tyr182) rabbit mAb (Cell Signaling, Beverly, MA, USA), p38 MAPK rabbit pAb (Cell Signaling, Beverly, MA, USA), Phospho-MAPKAPK-2 (Thr222) antibody (Cell Signaling, Beverly, MA, USA), MAPKAPK-2 antibody (Cell Signaling, Beverly, MA, USA), PUMA rabbit pAb (Abcam, Cambridge, MA, USA), Bak rabbit pAb (Cell Signaling, Beverly, MA, USA) and Cleaved Caspase-3 (Asp175) rabbit pAb (Cell Signaling, Beverly, MA, USA). The membranes were developed on a Kodak X-OMAT 2000A imaging system (Kodak, Rochester, NY, USA). The intensity of the protein band was analyzed by ImageJ software (1.46b, National Institutes of Health, USA). All Western blot analyses were performed in triplicate.
Phospho p53 ser15 rabbit pab
Manufactured by Cell Signaling Technology
Sourced in United States
Phospho-p53 (Ser15) rabbit pAb is a primary antibody that detects p53 protein phosphorylated at serine 15. It is suitable for Western Blotting applications.
Automatically generated - may contain errors
Lab products found in correlation
Phospho p38 mapk thr180 tyr182 rabbit mab, by Cell Signaling Technology (2 mentions)
β actin mouse mab, by Santa Cruz Biotechnology (2 mentions)
Siah1 rabbit pab, by Abcam (2 mentions)
P53 mouse mab, by Abcam (2 mentions)
P38 mapk rabbit pab, by Cell Signaling Technology (2 mentions)
Cleaved caspase 3 asp175 rabbit pab, by Cell Signaling Technology (2 mentions)
Puma rabbit pab, by Abcam (2 mentions)
Molecular imager chemidoc xrs, by Bio-Rad (1 mentions)
2 protocols using phospho p53 ser15 rabbit pab
1
Protein Profiling by Western Blotting
2
Western Blotting Analysis of Apoptosis-Related Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blotting was performed by standard protocols as described previously (Chen et al., 2013b (link); Sun et al., 2014 (link)). Proteins were probed with the following antibodies: SIAH1 rabbit pAb (Abcam, Cambridge, MA, United States), β-Actin mouse mAb (Santa Cruz, Santa Cruz, CA, United States), Phospho-p38 MAPK (Thr180/Tyr182) rabbit mAb (Cell Signaling Technology, Inc., Beverly, MA, United States), p38 MAPK rabbit pAb (Cell Signaling Technology, Inc., Beverly, MA, United States), Phospho-p53 (Ser15) rabbit pAb (Cell Signaling Technology, Inc., Beverly, MA, United States), p53 mouse mAb (Abcam, Cambridge, MA, United States), p53 upregulated modulator of apoptosis (PUMA) rabbit pAb (Abcam, Cambridge, MA, United States), Bcl-2 homologous antagonist/killer (Bak) rabbit pAb (Cell Signaling Technology, Inc., Beverly, MA, United States), and cleaved caspase-3 (Asp175) rabbit pAb (Cell Signaling Technology, Inc., Beverly, MA, United States). The membranes were developed on Molecular Imager ChemiDoc XRS + (Bio-Rad, Hercules, CA, United States), and the intensity of the protein band was analyzed by ImageJ software (1.48V, National Institutes of Health, United States). All Western blot analyses were performed in triplicate.
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