Mouse monoclonal anti rhoa

The mouse monoclonal anti-LRP6 antibody was from Santa Cruz (Santa Cruz, CA, USA). Rabbit polyclonal anti-p-LRP6 (Ser1490) was from Bioss (Woburn, MA, USA). Mouse monoclonal anti-β-catenin was from BD (Franklin Lakes, NJ, USA). Anti-phalloidin was from Life (Carlsbad, CA, USA). Mouse monoclonal anti-vinculin was from Boster (Wuhan, China). Mouse monoclonal anti-α-tubulin, acetyl-α-tubulin, tyrosine-α-tubulin were from Sigma-Aldrich (Saint Louis, MO, USA). Rabbit polyclonal anti-detyrosinated-α-tubulin, Mouse monoclonal anti-RhoA, Rac1and Cdc42 were purchased from Abcam (Cambridge, MA, USA). Rabbit polyclonal anti-phosphor-MAP1B (Thr1265) was from Novusbio (Littleton, CO, USA). Rabbit polyclonal anti-MACF1 was from Proteintech (Chicago, IL, USA). Rabbit monoclonal anti-phosphor-GSK3β (Ser9) and mouse monoclonal anti-GSK3β were from Cell Signaling (Beverly, MA, USA). Rabbit polyclonal anti-His, mouse monoclonal anti-His and anti-GST were from Origene (Rockville, MD, USA).

The following primary antibodies were used in this study: Mouse monoclonal anti-RHOG, mouse monoclonal anti-RHOA, rabbit polyclonal anti-RHOC, mouse monoclonal anti-RAC1, rabbit monoclonal anti-ROCK1, rabbit monoclonal anti-ROCK2, rabbit polyclonal anti-p-AKT1 (phospho-S473), rabbit polyclonal anti-pan-AKT, and rabbit polyclonal anti-Actin antibodies, as well as the MEK1/2 inhibitor U0126 and the PI3K inhibitor Wortmannin, which were obtained from Abcam (Abcam Inc., Cambridge, UK). Rabbit polyclonal anti-CDC42 (sc-87), mouse monoclonal anti-p-ERK1 (phospho-Y204), mouse monoclonal anti-ERK, and goat polyclonal anti-STARD13 antibodies were obtained from Santa Cruz (Santa Cruz Biotechnology., Santa Cruz, CA, US). Anti-rabbit and anti-mouse HRP-conjugated secondary antibodies were obtained from Promega (Promega Co., Maddison, WI, USA). The Dominant active Cherry-tagged RAC1-Q61L and Cherry-tagged CDC42-Q61L constructs were a generous gift from Dr. Louis Hodgson.