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μct50 scanner

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The Scanco μCT50 scanner is a high-resolution micro-computed tomography (micro-CT) system designed for non-destructive imaging and analysis of small samples. It provides three-dimensional visualization and quantitative data on the internal structure and composition of materials.

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Lab products found in correlation

Avizo 9, by Thermo Fisher Scientific (1 mentions) Matlab, by MathWorks (1 mentions) Amira 5, by Visage Imaging (1 mentions) Inveon ct scanner, by Siemens (1 mentions) Analyzepro, by AnalyzeDirect (1 mentions) Analyzepro 1, by AnalyzeDirect (1 mentions)

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μCT50 (208 citations) μCT50 specimen scanner (2 citations)

12 protocols using μct50 scanner

1

Quantify Bone Regeneration via μCT

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μCT analysis was performed to quantify the volume and quality of the new bone as previously described (Hu et al., 2017a (link); Cheng et al., 2020 (link)). Fracture tibias were dissected free of attached muscle 14 days post-fracture, fixed in 4% PFA and stored in 70% ethanol. Fracture calluses were analyzed in the UCSF Core Center for Musculoskeletal Biology (CCMBM, NIH P30 funded core) using the Scanco μCT50 scanner (Scanco Medical AG, Basserdorf, Switzerland) with 10 μm voxel size and X-ray energies of 55 kVp and 109 μA. A lower excluding threshold of 400 mg hydroxyapatite (HA)/mm3 was applied to segment total mineralized bone matrix from soft tissue in studies of control and treated mice. Linear attenuation was calibrated using a Scanco hydroxyapatite phantom. The regions of interest (ROI) included the entire callus without existing cortical clearly distinguished by its anatomical location and much higher mineral density. μCT reconstruction and quantitative analyses were performed to obtain the following structural parameters: volume fraction (bone volume/total volume as %), trabecular connective density as trabecular bifurcations (#/mm3), and bone mineral density (mg HA/cm3).
Rivera K.O., Cuylear D.L., Duke V.R., O’Hara K.M., Zhong J.X., Elghazali N.A., Finbloom J.A., Kharbikar B.N., Kryger A.N., Miclau T., Marcucio R.S., Bahney C.S, & Desai T.A. (2023). Encapsulation of β-NGF in injectable microrods for localized delivery accelerates endochondral fracture repair. Frontiers in Bioengineering and Biotechnology, 11, 1190371.
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2

Micro-CT Analysis of Fixed Samples

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Fixed samples were scanned using a micro‐CT (μCT) SCANCO μCT50 scanner (SCANCO Medical AG, Brüttisellen, Switzerland; V1.28) at the University of Southern California Molecular Imaging Center. The μCT images were captured at a resolution of 10 μm under an X‐ray source of 90 kVp and 78 μA. Three‐dimensional reconstruction was done using AVIZO 9.5 software (Thermo Fisher Scientific).
Wen Q., Jing J., Han X., Feng J., Yuan Y., Ma Y., Chen S., Ho T, & Chai Y. (2020). Runx2 Regulates Mouse Tooth Root Development Via Activation of WNT Inhibitor NOTUM. Journal of Bone and Mineral Research, 35(11), 2252-2264.
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3

Maxillary Bone Loss Quantification

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Maxillary samples were collected at 0, 1, 3, 6, and 10 days (Jiao et al., 2013 (link)) after surgery and scanned with a Scanco μCT50 scanner (Scanco Medical AG, Brutishly, Switzerland) with a resolution of 20 μm at 70 kVp and 200 μA. The 3D images from the buccal and palatal sides were constructed using Materialize Mimics v17.0 software. As for bone loss evaluation, the distance between the cemento-enamel junction (CEJ) and alveolar bone crest (ABC) was measured by the abovementioned software. Twelve sites from each maxillary molar were chosen for measurement, and averaged distances were calculated. Each measurement was repeated three times.
Li D., Feng Y., Tang H., Huang L., Tong Z., Hu C., Chen X, & Tan J. (2020). A Simplified and Effective Method for Generation of Experimental Murine Periodontitis Model. Frontiers in Bioengineering and Biotechnology, 8, 444.
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4

Analyzing Fracture Callus Mineralization

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μCT analysis was performed as previously described15 (link),85 (link). Fracture tibias were dissected free of attached muscle 14 days post-fracture, fixed in 4% PFA and stored in 70% ethanol. Fracture calluses were analyzed using the Scanco μCT50 scanner (Scanco Medical AG, Basserdorf, Switzerland) with 10 μm voxel size and X-ray energies of 55 kVp and 109 μA. A lower excluding threshold of 400 mg hydroxyapatite (HA)/mm3 was applied to segment total mineralized bone matrix from soft tissue in studies of control and β-NGF treated mice. Linear attenuation was calibrated using a Scanco hydroxyapatite phantom. The regions of interest (ROI) included the entire callus without existing cortical clearly distinguished by its anatomical location and much higher mineral density. μCT reconstruction and quantitative analyses were performed to obtain the following structural parameters: trabecular spacing (mm), trabecular number (#/mm), trabecular connective density as trabecular bifurcations (#/mm3), bone mineral density (mg HA/cm3), bone volume (as %), trabecular thickness (mm), and tissue mineral density (mg HA/cm3).
Rivera K.O., Russo F., Boileau R.M., Tomlinson R.E., Miclau T., Marcucio R.S., Desai T.A, & Bahney C.S. (2020). Local injections of β-NGF accelerates endochondral fracture repair by promoting cartilage to bone conversion. Scientific Reports, 10, 22241.
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5

Micro-CT Analysis of Alveolar Bone Loss

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Formalin-fixed maxillae were subjected to micro-CT image analysis. The specimens were scanned by Scanco μCT50 scanner (Scanco Medical AG, Brutishly, Switzerland) using a voxel size of 18 μm at 70k Vp and 200 μA. The Materialize Mimics v17.0 Software was used for reconstruction, visualization, and quantification. The length between the cementoenamel junction and alveolar bone crest (CEJ-ABC) at two sites for the first molars (distopalatal and distobuccal) and two sites for the second molars (mesiopalatal and mesiobuccal) in three-dimensional images was measured to assess alveolar bone loss (22 (link)). Cortical bone mineral density (BMD) was also assessed.
Zhang Y., Kuang W., Li D., Li Y., Feng Y., Lyu X., Huang G.B., Lian J.Q., Yang X.F., Hu C., Xie Y., Xue S, & Tan J. (2021). Natural Killer-Like B Cells Secreting Interleukin-18 Induces a Proinflammatory Response in Periodontitis. Frontiers in Immunology, 12, 641562.
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6

Micro-CT Analysis of Vertebral Microstructure

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The scanning protocol included an isometric resolution of 15 μm, with X-ray energy settings of 70 kV and 200 μA. The microstructure of the vertebrae was measured using a Scanco μCT50 scanner (Scanco Medical AG, Bassersdorf, Switzerland). Prior to histological processing, samples were fixed in paraformaldehyde and used for micro-CT. The scanned images from each group were evaluated at the same threshold to allow 3-dimensional structural reconstruction of each sample.
Zhao Y., Qiu C., Wang W., Peng J., Cheng X., Shangguan Y., Xu M., Li J., Qu R., Chen X., Jia S., Luo D., Liu L., Li P., Guo F., Vasilev K., Liu L., Hayball J., Dong S., Pan X., Li Y., Guo L., Cheng L, & Li W. (2020). Cortistatin protects against intervertebral disc degeneration through targeting mitochondrial ROS-dependent NLRP3 inflammasome activation. Theranostics, 10(15), 7015-7033.
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7

Microcomputed Tomography Analysis of Disc Height

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Microcomputed tomography (μCT) imaging was performed on mouse tails at 10 μm isotropic resolution (300 ms integration time, 55kVp, 72 microA) using a μCT50 scanner (Scanco; Brüttisellen, Switzerland). Volumetric calculations of disc height for experimental levels and intervening controls were performed in Matlab (V9.2.0; Mathworks; Natick, MA, USA) using a custom program adapted from a previous method.30 (link) Disc height was calculated as the distance between the superior endplate and inferior endplate averaged over the entire disc. Average proximal vertebral body height was measured in a similar fashion and defined as the sum of average superior endplate, inferior endplate, and mid-vertebral body measurements. To calculate disc height index (DHI), average disc height was divided by the average height of the proximal vertebral body.
Piazza M., Peck S.H., Gullbrand S.E., Bendigo J.R., Arginteanu T., Zhang Y., Smith H.E., Malhotra N.R, & Smith L.J. (2018). Quantitative MRI correlates with histological grade in a percutaneous needle injury mouse model of disc degeneration. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 36(10), 2771-2779.
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8

Micro-CT Analysis of Murine Rib Cage

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Scans of the mouse rib cage were performed at the USC’s Molecular Imaging Center on a subset of the animals (80-293 days post resection). μCT scans were acquired with the InveonCT scanner (Siemens Medical Solutions USA, Inc., Knoxville, TN) with the following settings: 80kVp, 120uA, no filter, 451 projections covering 220 degrees with 4s/projection, bin=2, and a voxel size of 18.729 microns. Data were reconstructed using Cobra Reconstruction Software (Exxim Computing Corp., Pleasanton, CA). One sample was scanned with higher resolution on the μCT 50 scanner (Scanco Medical AG, Bruttisellen, Switzerland) using the following settings: 70kVp, 114uA, no filter, 2000 projections covering 360 degrees with 1.5s/projection, and a voxel size of 6.8 microns. Higher resolution data were reconstructed using the Scanco software. Datasets were loaded into Amira 5.3.1 (Visage Imaging, Inc., Berlin, Germany) or Osirix (http://www.osirix-viewer.com) for visualization and analysis. Images were segmented to measure mineralized cartilage volumes (mm3) in areas of resection. Measurements of each sample were performed by at least two people blinded to the strain and healing time.
Srour M.K., Fogel J.L., Yamaguchi K.T., Montgomery A.P., Izuhara A.K., Misakian A.L., Lam S., Lakeland D.L., Urata M.M., Lee J.S, & Mariani F.V. (2015). Natural large-scale regeneration of rib cartilage in a mouse model. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 30(2), 297-308.
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9

Maxilla Samples µCT Imaging Protocol

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Maxilla samples were fixed in 4% PFA overnight followed by three washes in PBS. Samples were scanned on a SCANCO μCT50 scanner with 70 kVp voltage and a tube current of 114 μA at 6 μm isotropic voxel size. Scans were analysed by MicroView software.
Zhao J., Birjandi A.A., Ahmed M., Redhead Y., Olea J.V, & Sharpe P. (2022). Telocytes regulate macrophages in periodontal disease. eLife, 11, e72128.
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10

Quantifying Mineralized Tissue Properties

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Hemi-mandibles and forepaws were scanned in a μCT 50 scanner (Scanco Medical, Bassersdorf, Switzerland) at 70 kVp, 76 μA, 0.5 Al filter, 900 ms integration time, and 6 μm (mandible) or 10 μm (forepaw) voxel dimension. Reconstructed images were calibrated to 5 known densities of hydroxyapatite and analyzed using AnalyzePro (version 1.0; AnalyzeDirect, Overland Park, KS). Mineral density heat maps were generated for forepaws and mandibles. A threshold of 650 mg HA/cm3 was set for mineralized tissue of forepaws. Mandible regions of interest were defined from 480 μm forward from the mesial root and 480 μm backward to the distal root, using the most mesial and distal root points as landmarks. For mandibles, thresholds were set for enamel (1650 mg HA/cm3) and dentin/cementum/bone (650 mg HA/cm3) to determine enamel, dentin, and alveolar bone volumes and densities as previously described [24 (link)–26 (link)]. Measurements were performed in combined male and female animals as sex specific analysis did not alter the findings.
Zimmerman K., Li X., von Kroge S., Stabach P., Lester E.R., Chu E.Y., Srivastava S., Somerman M.J., Tommasini S.M., Busse B., Schinke T., Carpenter T.O., Oheim R, & Braddock D.T. (2022). Catalysis-independent ENPP1 protein signaling regulates mammalian bone mass. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 37(9), 1733-1749.
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