Chk2(A-12) mouse monoclonal antibody from Santa Cruz Biotechnology (sc-5278) was used. Staining with this antibody was performed in an OMNIS staining device (Dako). Antigen unmasking was performed with EnV Flex TRS high pH reagent no. REF GV804 (Dako) at 97 °C for 20 min. The sections were incubated for 30 min with the primary antibody diluted 1:50 with EnV Flex/HRP polymer (Dako, REF GV800).
For p53 mouse monoclonal antibody (DO7) (Cell Marque; REF 453M-95, catalog number CM-453M-95), staining was performed in an OMNIS staining device (Dako). Antigen unmasking was performed with EnV Flex TRS high pH reagent (Dako; no. REF GV804) at 97 °C for 20 min. The sections were incubated for 30 min with the primary antibody at a dilution of 1:500 with EnV Flex/HRP polymer (Dako, REF GV800).
For p53 mouse monoclonal antibody (DO7) (Cell Marque; REF 453M-95, catalog number CM-453M-95), staining was performed in an OMNIS staining device (Dako). Antigen unmasking was performed with EnV Flex TRS high pH reagent (Dako; no. REF GV804) at 97 °C for 20 min. The sections were incubated for 30 min with the primary antibody at a dilution of 1:500 with EnV Flex/HRP polymer (Dako, REF GV800).