Oridonin c20h28o6

Oridonin (C₂₀H₂₈O₆) and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Primary rabbit monoclonal antibodies (diluted at 1:1,000) against phosphorylated-LKB1, phosphorylated- AMPK, E-cadherin (E-Ca), N-cadherin(N-Ca) and β-actin were purchased from Cell Signaling Technology, Inc (Beverly, MA, USA). AICAR and Compound C, OE-LKB1

Oridonin (C₂₀H₂₈O₆) was obtained from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in dimethyl sulfoxide (DMSO). Stock solutions were stored at -20 °C. Antibodies against phosphorylated-Janus Kinase-2 (p-JAK2)(Tyr1007/1008, #3776, 1:1000), JAK2 (#3230, 1:1000), phosphorylated-Signal Transducer and Activator of Transcription 3 (p-STAT3)(Tyr705, #9145, 1:1000), STAT3 (#30835, 1:1000), E-cadherin (#14472, 1:1000), N-cadherin (#13116, 1:1000), Vimentin (#5741, 1:1000) and β-actin (#4970, 1:1000) were obtained from Cell Signaling Technology, Inc (Beverly, MA, USA). Antibody against VEGFA (#ab46154, 1:1000) was purchased from Abcam, Inc (Cambridge, Britain). The ELISA kit was gained from RayBiotech Inc (Norcross, GA, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was acquired from Sigma Chemical Co. (St. Louis, MO, USA).
Human thyroid cancer cell lines TPC-1, BCPAP, thyroid cell line Nthy-ori 3-1 and human umbilical vein endothelial cell line HUVEC were obtained from the American Type Culture Collection (Manassas, VA, USA). The cell lines were cultured in 1640 medium, which contains with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and seeded at 37 °C in a humidified incubator with 5% CO₂.