Alexa fluor 488 conjugated goat anti mouse

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom

Alexa Fluor 488-conjugated goat anti-mouse is a fluorescently labeled secondary antibody. It is designed to detect and bind to mouse primary antibodies, enabling visualization and quantification of target proteins or other biomolecules in various applications, such as immunoassays, flow cytometry, and microscopy.

Automatically generated - may contain errors

Lab products found in correlation

123 protocols using alexa fluor 488 conjugated goat anti mouse

Investigating Quantum Dot Effects on Cell Adhesions

Check if the same lab product or an alternative is used in the 5 most similar protocols

To analyze the effects of QDs on BEAS-2B and HFF-1 cell focal adhesions, confocal microscopy was conducted as previously described [25] . For this end, cells were incubated with primary anti-vinculin mouse monoclonal (no. ab18058, 1:200; Abcam, Cambridge, UK), secondary Alexa Fluor 488-conjugated goat anti-mouse (Molecular Probes, Leiden, Netherlands) and Alexa Fluor 546-conjugated phalloidin (Molecular Probes, Leiden, Netherlands) antibodies. High content image analysis using InCellanalyser 2000 was also conducted to examine morphological changes using primary anti-tubulin (Abcam, Cambridge, UK), secondary Alexa Fluor 488-conjugated goat anti-mouse (Molecular Probes, Life Technologies Europe, BV, Belgium), and AF568-coupled phalloidin (Molecular Probes, Life Technologies Europe, BV, Belgium) antibodies (For more details see supplementary material).

Manshian B.B., Abdelmonem A.M., Kantner K., Pelaz B., Klapper M., Nardi Tironi C., Parak W.J., Himmelreich U, & Soenen S.J. (2016). Evaluation of quantum dot cytotoxicity: interpretation of nanoparticle concentrations versus intracellular nanoparticle numbers. Nanotoxicology, 10(9).

+ Open protocol

+ Expand

Immunofluorescence Analysis of CXXC5 and β-catenin

Check if the same lab product or an alternative is used in the 5 most similar protocols

HaCaT cell lines were seeded in a 12-well plate on coverslips. Cultured cells were washed with PBS and fixed with 10% formalin (Sigma-Aldrich), and then permeabilized with 0.2% Triton X-100. After blocking with 5% BSA in PBS, the cells were blotted with primary antibodies: mouse anti-CXXC5 (Santa Cruz Biotechnology, 1:20) or rabbit anti-β-catenin (Abcam, 1:20) overnight at 4 °C. After washing with PBS, the cells were blotted with appropriate secondary antibodies: Alexa Fluor 488-conjugated goat anti-mouse or Alexa Fluor 555-conjugated goat anti-rabbit (Molecular Probes, Leiden, the Netherlands) for 1 h at room temperature and counterstained with DAPI. Images were taken using an LSM510 confocal microscope (Carl Zeiss Inc.). The fluorescence intensity was quantified using Zen software V3.1 (Carl Zeiss Inc.)

Ryu Y.C., Park J., Kim Y.R., Choi S., Kim G.U., Kim E., Hwang Y., Kim H., Han G., Lee S.H, & Choi K.Y. (2023). CXXC5 Mediates DHT-Induced Androgenetic Alopecia via PGD2. Cells, 12(4), 555.

+ Open protocol

+ Expand

Immunofluorescence Analysis of TNBC

Check if the same lab product or an alternative is used in the 5 most similar protocols

The TNBC tissue microarray was incubated with a specific primary antibody at 4 °C overnight. The cells were then incubated for 1 h with Alexa Fluor 488-conjugated goat anti-mouse and Alexa Fluor 647-conjugated donkey anti-rabbit secondary antibodies (Molecular Probes). Nuclei were counterstained with 0.1 μg/mL DAPI (Sigma). Total fluorescence intensity was examined and analyzed using the Pannoramic MIDI scanner from “3DHISTECH”.

Li X., Zhou D., Cai Y., Yu X., Zheng X., Chen B., Li W., Zeng H., Hassan M., Zhao Y, & Zhou W. (2022). Endoplasmic reticulum stress inhibits AR expression via the PERK/eIF2α/ATF4 pathway in luminal androgen receptor triple-negative breast cancer and prostate cancer. NPJ Breast Cancer, 8, 2.

+ Open protocol

+ Expand

Immunofluorescent Localization of hPML Isoforms

Check if the same lab product or an alternative is used in the 5 most similar protocols

PML-KO cells stably transduced with FLAG-tagged hPML-I to VI isoforms or WT MEFs were seeded on glass coverslips placed in 3.5-cm wells. After 24 h, the cells were permeabilized and fixed for 10 min in Triton X-100/4 % formaldehyde at room temperature (RT), followed by four washes with PBS. The cells were then treated with 10 % goat serum (Sigma) for 30 min at RT followed by 4 h of incubation with antibodies against FLAG (Sigma, 1:150) or hPML (Santa Cruz, 1:150) or mPML (Enzo Life Sciences, 1:150) in 10 % goat serum at RT. They were then washed four times with PBS and fluorescently stained with Alexa Fluor 488-conjugated goat anti-mouse (Molecular Probes, Eugene, OR) diluted 1:100 in 10 % goat serum for 1 h at RT. The cells were then washed 4 times with PBS before mounting in Vectashield (Vector Laboratories, Peterborough, UK). Hoechst 33342 (0.8 μg/ml; Molecular Probes) was added along with the penultimate PBS wash to reveal DNA. Z-stacks were acquired on an AxioObserver Microscope (Carl Zeiss Canada, Toronto, ON) equipped with the Apotome module, and the median optical slice of each Z-stack was analyzed.

Masroori N., Merindol N, & Berthoux L. (2016). The interferon-induced antiviral protein PML (TRIM19) promotes the restriction and transcriptional silencing of lentiviruses in a context-specific, isoform-specific fashion. Retrovirology, 13, 19.

+ Open protocol

+ Expand

Immunocytofluorescence and oxLDL Uptake Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunocytofluorescence, LECs were fixed with 4% paraformaldehyde and blocked with 5% normal goat serum. Next, cells were incubated with rabbit anti-mouse podoplanin (Sigma Chemical, St Louis, Missouri, USA) for 1 h in a 1:200 dilution. The respective secondary antibody was Alexa Fluor 488-conjugated goat anti-mouse (Molecular Probes, Invitrogen, San Diego, California, USA). In addition, 4′,6-diamidino-2-phenylindole (DAPI, Vectashield, Vector laboratories, Burlingame, Ca) was used to counterstain cell nuclei. Immunofluorescence was visualized with an immunofluorescence microscope system (Nikon Eclipse E600, Kawasaki, Kanagawa, Japan).
For the uptake of oxiLDL, cells were incubated with Alexa Fluor 488-conjugated AcLDL (Thermo Fisher, Waltham, MA, USA) at a concentration of 10 µg/mL for 3 h. Then, cells were washed three times with PBS and fixed with 4% paraformaldehyde. Fluorescence was visualized with an immunofluorescence microscope system (Nikon Eclipse E600, Kawasaki, Kanagawa, Japan).

Ribera J., Portolés I., Córdoba-Jover B., Rodríguez-Vita J., Casals G., González-de la Presa B., Graupera M., Solsona-Vilarrasa E., Garcia-Ruiz C., Fernández-Checa J.C., Soria G., Tudela R., Esteve-Codina A., Espadas G., Sabidó E., Jiménez W., Sessa W.C, & Morales-Ruiz M. (2021). The loss of DHX15 impairs endothelial energy metabolism, lymphatic drainage and tumor metastasis in mice. Communications Biology, 4, 1192.

+ Open protocol

+ Expand

Immunofluorescence Staining of Cellular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were cultured in glass-bottom plates, fixed in 3% paraformaldehyde, and then permeabilized with Tris-buffered saline containing 0.3% Triton X-100. Nonspecific recognition was blocked with Blocking One (Nacalai Tesque). The permeabilized cells were incubated with 1 µg/mL primary Ab overnight at 4 °C and subsequently incubated with Alexa Fluor 488-conjugated goat anti-mouse, goat anti-rabbit, or goat anti-rat Abs (Molecular Probes). Nuclei were visualized using DAPI (Molecular Probes). For actin filament staining, rhodamine phalloidin (Molecular Probes) was used. Immunofluorescence images were obtained using the Leica TCS SPE microscope system (Leica Microsystems). The following primary Abs were used: mouse anti-human CD9 (MM2/57; Invitrogen), mouse anti-human CD81 (JS64; BECKMAN COULTER), mouse anti-human SIRT1 (1F3; CST), rabbit anti-cleaved caspase-3 (CST), and rabbit anti-Ki-67 (D3B5; CST).

Jin Y., Takeda Y., Kondo Y., Tripathi L.P., Kang S., Takeshita H., Kuhara H., Maeda Y., Higashiguchi M., Miyake K., Morimura O., Koba T., Hayama Y., Koyama S., Nakanishi K., Iwasaki T., Tetsumoto S., Tsujino K., Kuroyama M., Iwahori K., Hirata H., Takimoto T., Suzuki M., Nagatomo I., Sugimoto K., Fujii Y., Kida H., Mizuguchi K., Ito M., Kijima T., Rakugi H., Mekada E., Tachibana I, & Kumanogoh A. (2018). Double deletion of tetraspanins CD9 and CD81 in mice leads to a syndrome resembling accelerated aging. Scientific Reports, 8, 5145.

+ Open protocol

+ Expand

Subcellular Localization of HA-RPL11 under miR-101-3p Overexpression

Check if the same lab product or an alternative is used in the 5 most similar protocols

The U2OS cells were co-transfected with HA-tagged RPL11 plasmid and miR-101-3p mimic using Lipofectamine 2000^TM reagent. After incubation for 48 h, the cells were fixed with 100% ice-cold methanol for 10 min, and then permeabilized in PBS containing 0.25% Triton X-100 for 10 min. After washing, the cells were stained with anti-HA (sc-7392, Santa Cruz) overnight at 4 °C, followed by Alexa Fluor 488-conjugated goat anti-mouse (Molecular Probes, Eugene, OR, USA) for 1 h at room temperature. The nucleolus was stained with anti-fibrillarin (ab5821, Abcam), and DNA was detected by DAPI (Sigma-Aldrich). The fluorescence response HA-RPL11 and fibrillarin staining cells were visualized using the Nikon ECLIPS Ti-U fluorescence microscope (Nikon, Tokyo, Japan) and analyzed using NIS-Elements imaging software (Nikon, Tokyo, Japan, ver 4.20).

Park J., Cho M., Cho J., Kim E.E, & Song E.J. (2022). MicroRNA-101-3p Suppresses Cancer Cell Growth by Inhibiting the USP47-Induced Deubiquitination of RPL11. Cancers, 14(4), 964.

+ Open protocol

+ Expand

Immunofluorescence Quantification of DNA Repair Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Indirect immunofluorescence was carried out essentially as described (40 (link)). The primary antibodies used were: FANCD2 (Abcam, ab2187, 1:4000), CtIP (mouse monoclonal, 1:400) and RAD51 (Calbiochem, PC130, 1:1000). The secondary antibodies used were: Alexa Fluor 594-conjugated goat anti-rabbit (1:1000) and Alexa Fluor 488-conjugated goat anti-mouse (1:1000; Molecular Probes). For statistical analysis of nuclear foci formation, images were taken using a Leica DM LB2 microscope with a Hamamatsu Orca-ER camera. Quantification of CtIP and RAD51 foci was carried out using ImageJ.

Thompson E.L., Yeo J.E., Lee E.A., Kan Y., Raghunandan M., Wiek C., Hanenberg H., Schärer O.D., Hendrickson E.A, & Sobeck A. (2017). FANCI and FANCD2 have common as well as independent functions during the cellular replication stress response. Nucleic Acids Research, 45(20), 11837-11857.

+ Open protocol

+ Expand

Immunofluorescent Imaging of Integrin Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

HASM cells were serum starved for 48 hours followed by Af pretreatment for the indicated times. Lysates were prepared from cells, lungs, or BAL fluid using radioimmunoprecipitation (RIPA) lysis buffer supplemented with protease inhibitor and phosphatase inhibitor cocktails (Roche) and sonication. Primary antibody staining was detected using near-infrared conjugated secondary antibodies and quantified with the LiCor Odyssey Imaging System and ImageStudio software (LiCor Biosciences). For immunofluorescent staining, cells were fixed in 3.7% paraformaldehyde and permeabilized with PBS containing 0.2% vol/vol Triton X-100. Cells were then blocked in 2% (wt/vol) BSA in PBS. Cells were incubated with mouse anti-vinculin antibody (1:100, Sigma) or rabbit anti-α9 integrin (Santa Cruz, 1:75) overnight at 4°C in blocking buffer. Cells were then incubated with AlexaFluor488-conjugated goat anti-mouse or goat anti-rabbit (1:200) secondary antibody followed by staining with Texas Red-conjugated phalloidin (Molecular Probes). Cells were mounted on coverglass with ProLong® Gold antifade reagent with DAPI. Images were obtained using the 63x oil immersion (NA 1.4, zoom 2) objective of a Leica SP5 confocal microscope and analyzed using Imaris software (BitPlane).

Balenga N.A., Klichinsky M., Xie Z., Chan E.C., Zhao M., Jude J., Laviolette M., Panettieri RA J.r, & Druey K.M. (2015). A fungal protease allergen provokes airway hyperresponsiveness in asthma. Nature communications, 6, 6763.

+ Open protocol

+ Expand

Flow Cytometry of Neural Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

FACS was performed as previously reported [1 (link),2 (link)]: The primary antibodies used were anti-tyrosine-hydroxylase (TH) monoclonal antibody (1:100, ab129991, Abcam) and anti-βIII-tubulin mouse monoclonal antibody (1:500) The secondary antibodies used were goat anti-mouse IgG H&L phycoerythrin (1:500, ab97041, Abcam) and Alexa Fluor^® 488-conjugated goat anti-mouse (1:500, A11001, Molecular Probes). The cells were analyzed by FACS Verse (BD Bioscience, San Jose, CA, USA), using FACS suiteTM software (BD Bioscience). FACS analyses were repeated in triplicate.

Yamane M., Takaoka N., Obara K., Shirai K., Aki R., Hamada Y., Arakawa N., Hoffman R.M, & Amoh Y. (2021). Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Can Extensively Differentiate to Tyrosine-Hydroxylase-Expressing Dopamine-Secreting Neurons. Cells, 10(4), 864.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!