Bcl 2

Manufactured by Cusabio

Sourced in United States, China

Bcl-2 is a lab equipment product that functions as a key regulator of apoptosis, or programmed cell death, in various cell types. It plays a critical role in maintaining the balance between cell survival and cell death.

Automatically generated - may contain errors

Lab products found in correlation

Caspase 3, by Cusabio (5 mentions) Bcl2 associated x (bax), by Cusabio (3 mentions) C myc, by Cusabio (3 mentions) Il 1β, by Cusabio (3 mentions) Interleukin 6 (il 6), by Cusabio (3 mentions) K ras, by Cusabio (2 mentions) Tnf α, by Cusabio (2 mentions) Il 10, by Cusabio (2 mentions) Bcl2 associated x (bax), by Abcam (1 mentions) Casp3c 1kt, by Cusabio (1 mentions) Il 1β, by Abcam (1 mentions) Glutathione (gsh), by Merck Group (1 mentions) Epigallocatechin gallate (egcg), by Merck Group (1 mentions) Cisplatin, by Merck Group (1 mentions) N acetyl cysteine (nac), by Merck Group (1 mentions) 5 fluorouracil, by Merck Group (1 mentions) Camptothecin, by Merck Group (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Cb 839, by Selleck Chemicals (1 mentions)

7 protocols using bcl 2

Apoptosis Protein Level Quantification

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The levels of the pro-apoptotic proteins (Bax (BioVision, Inc. (San Francisco, CA, USA) Catalog # E4513) and caspase-3 (Sigma-Aldrich (Saint-Louis, MO, USA) Catalog # CASP3C-1KT)) and the anti-apoptotic protein (Bcl-2, Cusabio (Wuhan, China) Catalog # CSB-E08854r) in the cortical homogenate were estimated using commercial kits according to the manufacturer’s protocols.

S. Yousef A.O., A. Fahad A., Abdel Moneim A.E., Metwally D.M., El-khadragy M.F, & Kassab R.B. (2019). The Neuroprotective Role of Coenzyme Q10 Against Lead Acetate-Induced Neurotoxicity Is Mediated by Antioxidant, Anti-Inflammatory and Anti-Apoptotic Activities. International Journal of Environmental Research and Public Health, 16(16), 2895.

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Hepatoprotective Effects of Antioxidants

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The dimethyl nitrosamine (DMN; C₂H₆N₂O; 98% purity) used in this study was produced by Sigma Chemical Co., Saint Louis, MO, USA. Syringic acid (SYRA; 98% purity; C₉H₁₀O₅) is produced by AK Scientific, USA. Tablets of ascorbic acid are produced by Kunimed Pharmachem Ltd, Ikeja, Lagos, Nigeria. Enzyme linked immunosorbent assay (ELISA) kits for the quantification of hepatic levels of TNF-α, Bax, IL-1β, Bcl-2, and caspase-3 were produced by Cusabio Technology Llc, Houston, TX, USA. Primers (Oligonucleotide sequence) for NF-κB and β-actin for the reverse transcriptase-polymerase chain reaction (RT-PCR) analyses were designed by Shanghai ShineGene Molecular Bio-Technologies, Inc., China.

Adeyi O.E., Somade O.T., Ajayi B.O., James A.S., Adeyi A.O., Olayemi Z.M, & Tella N.B. (2023). Syringic acid demonstrates better anti-apoptotic, anti-inflammatory and antioxidative effects than ascorbic acid via maintenance of the endogenous antioxidants and downregulation of pro-inflammatory and apoptotic markers in DMN-induced hepatotoxicity in rats. Biochemistry and Biophysics Reports, 33, 101428.

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Hepatoprotective Effects of EGME

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EGME (C₃H₈O₂; CAS# 109-84-4; 99.5% purity), is a product of BDH Laboratory Supplies, Poole, BH15 1TD, England. Rats IL-10, IL-6, TNF-α, IL-1β, caspase-3, p53, Bax, Bcl-2, c-Myc, and K-Ras enzyme-linked immunosorbent assay (ELISA) kits are manufactured by Cusabio Technology Llc, Houston, TX, USA. The rest of the reagents and chemicals used were obtained from a recognized chemical manufacturing company and were of standard and analytical grade.

Somade O.T., Ajayi B.O., Adeyi O.E., Adeshina A.A., James A.S, & Ayodele P.F. (2020). Ethylene glycol monomethyl ether-induced testicular oxidative stress and time-dependent up-regulation of apoptotic, pro-inflammatory, and oncogenic markers in rats. Metabolism Open, 7, 100051.

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Evaluation of Inflammatory Markers in Rats

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MC (C₃H₈O₂; CAS# 109-84-4; 99.5 % purity), is a product of BDH Laboratory Supplies, Poole, BH15 1TD, England. Rats TNF-α, IL-1β, IL-6, IL-10, caspase 3, p53, Bax, Bcl-2, c-Myc, and K-Ras enzyme-linked immunosorbent assay (ELISA) kits are products of Cusabio Technology Llc, Houston, TX, USA. Reagents/chemicals used in this study were of accepted grade, products of BDH Chemical Ltd, Poole, England or Sigma Chemical Co., Saint Louis, MO, USA.

Somade O.T., Ajayi B.O., Olushola M.O, & Omoseebi E.O. (2020). Methyl cellosolve-induced renal oxidative stress and time-dependent up-regulation of pro-inflammatory cytokines, apoptotic, and oncogenic markers in rats. Toxicology Reports, 7, 779-787.

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Hepatic and Renal Inflammatory Markers

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Hepatic and renal levels of interleukin-1beta (IL-1β), tumor necrosis factor-alpha (TNF-α) (Abcam, Cambridge, MA, USA), Bcl-2, caspase-3 and nuclear factor kappa B (Cusabio^®, Wuhan, China) were determined using previously listed highly specific ELISA-based kits.

Gad El-Karim D.R., Lebda M.A., Alotaibi B.S., El-kott A.F., Ghamry H.I, & Shukry M. (2023). Lutein Modulates Oxidative Stress, Inflammatory and Apoptotic Biomarkers Related to Di-(2-Ethylhexyl) Phthalate (DEHP) Hepato-Nephrotoxicity in Male Rats: Role of Nuclear Factor Kappa B. Toxics, 11(9), 742.

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Comprehensive Antibody and Compound Panel

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The following antibodies were used: GLS (#ab93434, Abcam, Cambridge, MA, USA), AREG (#sc74501, Santa Cruz Biotechnology, Dallas, USA), MLX (#85570 s, Cell signaling, Danvers, MA, USA), Lamin B1 (#12586 s, Cell signaling), caspase 3 (#9664 s, Cell signaling), GAPDH (#CSB-PA00025A0Rb, Cusabio biotech, Wuhan, China), Bcl-2 (#CSB-PA000983, Cusabio biotech) and β-actin (#ABT264, Sigma, St. Louis, MO, USA). AOA, EGCG, DMKG, NAC, glutathione, DOX, cisplatin, camptothecin, ETS, and 5-fluorouracil were purchased from Sigma. BPTES was purchased from Cayman Chemical (Ann Arbor, MI, USA). CB-839 was obtained from Selleckchem (Houston, TX, USA). shRNAs for AREG (SHCLNDTRCN0000117992 and SHCLNDTRCN0000117996) were purchased from Sigma. shRNAs for GLS were described previously [10 (link)].

Hwang S., Yang S., Kim M., Hong Y., Kim B., Lee E.K, & Jeong S.M. (2021). Mitochondrial glutamine metabolism regulates sensitivity of cancer cells after chemotherapy via amphiregulin. Cell Death Discovery, 7, 395.

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Cytokine and Apoptosis Protein Assay Protocol

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2-ME (C₃H₈O₂; CAS# 109-84-4; 99.5% purity), is a product of BDH Laboratory Supplies, Poole, BH15 1TD, England. Rats TNF-α (CSB-E11987r), IL-1β (CSB-E08055r), IL-6 (CSB-E04640r), IL-10 (CSB-E04595r), caspase-3 (CSB-E08857r), p53 (CSB-E08336r), Bax (CSB-EL002573RA), Bcl-2 (CSB-E08854r), c-Myc (CSB-E09260h), and K-Ras (CSB-EL012493h) enzyme-linked immunosorbent assay (ELISA) kits are products of Cusabio Technology Llc, Houston, TX, USA. All other used chemicals and reagents were of analytical grade and were products of Sigma Chemical Co., Saint Louis, MO, USA or BDH Chemical Ltd, Poole, England.

Somade O.T., Ajayi B.O., Olunaike O.E, & Jimoh L.A. (2020). Hepatic oxidative stress, up-regulation of pro-inflammatory cytokines, apoptotic and oncogenic markers following 2-methoxyethanol administrations in rats. Biochemistry and Biophysics Reports, 24, 100806.

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