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32 protocols using eos 450d

1

Photographic Documentation of an Insect

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The only specimen examined was found when searching for insects sitting on a wall of a house in the Morávka village (Fig. 2) in the Moravskoslezské Beskydy Mts in the NE part of the Czech Republic (Fig. 1). The specimen was photographed alive (see Figs 3,6) by means of a digital camera Canon EOS 450D with a macro lens Canon MP-E 65 mm 1-5× and ring macro flash Canon MR-14EX. Because the insect moved rapidly is was immediately captured for further study. The specimen was caught into a vial and killed by ethyl acetate. The dead dry specimen was subsequently photographed by means of a modified ore microscope with an attached body of Canon EOS 450D. It was manually repositioned upwards between each exposure and the final photographs were compiled from multiple layers (30-35) using Helicon Focus 7. The obtained images were edited in Adobe Photoshop CS6. After photography the specimen was put into a vial with 70% ethanol. Identification was done by the first author. The voucher specimen is deposited in SMOC -Slezské zemské muzeum, Opava, Czech Republic.
Abbreviations: AOL -minimum distance between posterior ocellus and anterior ocellus; F1, F2, etc. -funicle segments one, two, etc.; FV -minimum width of frontovertex; POL -minimum distance between posterior ocelli.
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2

Erythrocyte Suspension Microscopy

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5 μL of erythrocyte suspension was placed into observation chamber composed of two cover glasses which were glued together with nail polish. Samples were observed under Olympus GWB BH-2 (Olympus Corporation, Tokyo, Japan) microscope with phase contrast optics at objective magnification 400×. Images were taken with Canon EOS 450D digital camera (Canon Inc., Tokyo, Japan).
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3

Priapism Monitoring in Stroke Mice

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The mice were observed every day after the pMCAO procedure. The time of priapism occurrence and the recovery of flaccidity (disappearance of priapism) were both recorded. Photographs were taken using a Canon digital camera (Canon EOS 450D).
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4

Blinded Clinical Evaluation of Dental Implants

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All patients were recalled for baseline, and 1‐, 3‐ and 5‐year follow‐up examinations, and the clinical trial ended after the last follow‐up as the quality control of treatments was completed 5 years from the prosthetic treatment was performed. The clinical and radiographic assessments were blinded and performed by one observer, who was not involved in the treatment of the patients. Clinical photographs of the restorations, including the neighboring teeth and marginal peri‐implant mucosa, were taken by using a digital camera (Canon EOS450D, Canon) with a macro lens flash (MACRO RING LITE MR‐14 EX, Canon) at all follow‐up examinations. Digital intraoral radiographs (Digora® Optime digital films, Soredex, Tuusula, Finland) were obtained by using long cone paralleling technique with Eggen’s film holders at each follow‐up examination, and were assessed using the Digora Optime system. Biological, technical and aesthetic variables were registered at all examinations. Trial outcome measures were not changed after the trial commenced.
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5

Standardized Whole-Body Photography

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Before and after each sunscreen application participants had a series of standardized pictures of the whole body taken at fixed distances. We used a digital camera (Canon EOS 450D, EF-S 18–55 mm, Canon Inc., Japan) with an aperture of F:5.6, ISO of 1600 and shutter speed of 1/15. The participants were photographed in a black box with full-height fluorescent tubes (TL08, Philips, The Netherlands) emitting primarily UV-A radiation but also some visible light up to a wavelength of 410 nm. The pictures were assessed as blue channel pictures using GIMP version 2.8.14 (www.gimp.org).
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6

Imaging Techniques for Fossil Analysis

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Fossils were examined under Nikon SMZ 745 T or Zeiss SteREO Discovery.V20 stereomicroscopes. Macrophotography was undertaken using a digital camera Canon EOS 550D (Canon Inc., Tokyo, Japan) coupled with MP-E 65 mm or FE-S 50 mm macro photo lenses, or linked to a Nikon SMZ 745 T microscope illuminated by a Schott spotlight. The photograph of Pal cl 4 was taken with a Canon EOS 450 D and EF-S 60 mm macro photo lens under a film of ethanol. The photographs were processed using the image-editing software Adobe Photoshop CS and those taken at different distances were focus stacked using the stacking software Helicon focus Pro 6.2.2 (Helicon Soft Ltd., Kharkov, Ukraine) or Zerene Stacker (Zerene systems LLC, Richland, U.S.A.). Composed image of the Idoptilus sp. was obtained from digital microscope Keyence VHX VH-Z20UR located at the National Museum in Prague.
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7

Binocular Movement Testing Protocol

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Binocular movement testing was performed by a single experienced ophthalmologist to assure consistency in assessment. A digital camera (Canon EOS 450D, Canon Corporation, Tokyo, Japan) was placed 1m away in front of the patient at eye level. A circular marker with 10 mm in diameter was attached to the forehead of the patient as a reference. The patient was asked to follow an object presented by the examiner and photographs were taken in diagnostic positions of gaze, in accordance with standard clinical practice (7 (link)). Verbal encouragement was given to ensure stability of the patient’s head and maximum effort toward the extremes of gaze.
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8

Quantifying Arabidopsis Leaf Rosette Area

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The leaf area analysis was used to compare the leaf rosettes of control and Vl43‐inoculated Arabidopsis mutant and wild‐type plants. The plants were grown and infected in a 60‐mm pot. After infection, the plants were photographed once a week in a precisely defined distance with a digital camera (Canon EOS 450D). The program WinCAM 2004a (Regent Instruments) was used to analyse the leaf area. A 1 cm × 1 cm white square served as scale to calculate the pixel area of the visible surface.
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9

Maxillary Arch Measurements Protocol

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Plaster models of the maxillary dentition were oriented with the occlusal plane parallel to the horizontal plane. A standardized picture of the maxillary arch was then taken with a digital camera (Canon EOS 450D, Canon Inc., Tokyo, Japan), which was fixed on a tripod at a stable distance from the plaster models, at a 90° angle to the occlusal surface.
On the photographs, perpendicular lines to the mid palatal suture were drawn from the interproximal contacts between the first and second premolars on both sides, and from the most medial end of the third palatal ruga. Then the distance between line 'B' and the third palatal ruga was measured on each side. When line 'B' was located posteriorly to the third palatal ruga, a negative value was recorded for the distance; when it was anteriorly, a positive value was recorded (Figure 3).
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10

Rabbit Dental Model Measurements

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Mandibular dental models of all rabbits were made from elastomeric impressions taken immediately after sacrifice. The amount of tooth movement was measured using Adobe Photoshop software (Adobe Systems Inc., San Jose, CA, USA) from the center of the distal surface of the first PM to the center of the mesial surface of the second PM on standardized photographs of the dental models with a ruler (to scale the photo into a 1:1 ratio) taken by a Canon camera (EOS 450D; Canon, Toyko, Japan).
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