HMGB1 in mice serum was quantitated by a Mouse HMGB1 ELISA Kit (S203674, D&B Biological Science and Technology, Shanghai, China) according to the manufacturers’ instructions. MRX II microplate reader (Dynex, Chantilly, VA, United States) was used to measure OD values for each well at 450 nm.
Mrx 2 microplate reader
Manufactured by Dynex
Sourced in United States
The MRX II microplate reader is a compact and versatile instrument designed for a wide range of absorbance-based assays. It features a temperature-controlled incubator, continuous orbital shaking, and a high-precision optical system to provide accurate and consistent results across multiple microplates.
Automatically generated - may contain errors
Lab products found in correlation
Cell counting kit 8 (cck8), by Dojindo Laboratories (9 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (6 mentions)
Cck 8, by Dojindo Laboratories (5 mentions)
Cell counting kit 8 cck 8 assay, by Dojindo Laboratories (5 mentions)
Cck 8 solution, by Dojindo Laboratories (5 mentions)
Cck 8 assay, by Dojindo Laboratories (3 mentions)
Cell counting kit 8 cck 8 solution, by Dojindo Laboratories (3 mentions)
Click it 5 ethynyl 20 deoxyuridine edu imaging kit, by Thermo Fisher Scientific (3 mentions)
Click it edu imaging kit, by Thermo Fisher Scientific (2 mentions)
Hrp conjugated goat anti mouse ige, by Bio-Rad (2 mentions)
Tmb substrate kit, by BD (2 mentions)
Cck 8, by Dynex (2 mentions)
Infliximab, by Johnson & Johnson (2 mentions)
N 1 napthylethylenediamine dihydrochloride, by Merck Group (2 mentions)
Phosphoric acid, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Anti mouse ige, by BioLegend (1 mentions)
Cell counting kit 8 cck 8, by Merck Group (1 mentions)
Wst 8, by Dojindo Laboratories (1 mentions)
Calcusyn software, by Biosoft (1 mentions)
62 protocols using mrx 2 microplate reader
1
Quantifying HMGB1 in Mouse Serum
2
Evaluating OSCC Cell Viability
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OSCC cells or OSCC cells transfected with specific siRNA were cultured on 96-well plates (3×103 cells/well) and allowed to attach for 12 h, serum starved overnight to synchronize the cells, then the culture media was changed to the whole medium including the Doxorubicin or Doxorubicin combined with GC7 at indicated doses for addition 48 h. Next, cell counting kit-8 (10 μL/well, Dojindo, Kumamoto, Japan) was used following the protocol. Briefly, the cells with CCK-8 solution were incubated at 37 ℃ for 3 h, and absorbency was detected via the MRX II microplate reader (Dynex, Chantilly, VA) at 450 nm. Cell viability was estimated as percentages of blank control cells. Click-iT EdU Imaging Kit (Invitrogen, Carlsbad, CA) was used to measure the inhibitive ratio of cell viability following the product protocol.
3
Cell Proliferation Assay Using CCK-8
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Cell Counting Kit-8 (CCK-8; Dojindo Molecular Technologies, inc., Kumamoto, Japan) was used to estimate the cell number according to the manufacturer's protocol. Briefly, the same number of SUNE-1 cells (5×105/well) different levels of ACKR4 expression was seeded in 96-well plates. The number of cells in each well was counted at 0, 6, 12, 24 and 48 h after seeding. At these time points, CCK-8 solution was added and incubated for 30 min at 37°C, and then the absorbance at 450 nm was measured by an MRX II microplate reader (Dynex Technologies, Chantilly, VA, USA).
4
Assessing LUAD Cell Viability
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The cell viability of LUAD cells was detected with Cell Counting Kit-8 (CCK-8, Dojindo, Japan). First, 5×103 cells/well was suspended and cultured for 0 to 72 h. After that, 10 µL of CCK-8 solution was added, and MRX II microplate reader (Dynex Technologies, USA) was used to examine the absorbance of each well at 450 nm.
5
Measuring Total IgE Antibody in Serum
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Serum was assayed for total IgE antibody production. 96 well plates were coated with purified anti-mouse IgE (2 ug/ml, Biolegend, Clone: RME-1) in 0.05 M carbonate/bicarbonate buffer and incubated overnight at 4 °C. Following coating, plates were washed in PBS-Tween and non-specific binding blocked with 3% BSA (Sigma-Aldrich) in PBS for 1 hour at room temperature. Plates were washed and diluted serum (1:10) added to the plate and incubated for 2 h at 37 °C. After washing HRP conjugated goat anti-mouse IgE (1 ug/ml; BioRad) was added to the plates for 1 h. Finally, plates were washed and developed with TMB substrate kit (BD Biosciences, Oxford, UK) according to the manufacturer’s instructions. The reaction was stopped using 0.18 M H2SO4, when sufficient colour had developed. The plates were read by a MRX II microplate reader (DynexTechnologies, VA, USA) at 450 nm, with reference of 570 nm subtracted.
6
Radiation Effects on Cell Proliferation
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CCK-8 assay measured the proliferation capacity of cells. Transfected cells were seeded into 96-well culture dishes with a density of 3 × 103 cells/well for 12-h culture with or without 4Gy radiation treatment at the dose rate of 200 cGy/min using 137Cs γ-ray source (Atomic Energy of Canada Ltd, Mississauga, Ontario, Canada). Then, cell counting kit-8 (10 μl/well, Dojindo, Kumamoto, Japan) was added according to manufacturer’s protocol, followed by incubation at 37 °C for another 3 h. Absorbance at 450 nm was detected by the MRX II microplate reader (Dynex, Chantilly, VA, USA).
7
Quantifying Doxorubicin, Salinomycin, and PKF 118-310 Cytotoxicity
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Cell viability was measured using Cell Counting Kit-8 (CCK-8; Dojindo; Kumamoto, Japan) following the manufacturer's instructions. HCC cells were plated into 96-well plates (3000 cells/well; 100μl media). Following overnight cultured, the media was removed and replaced with conditioned media containing different concentrations of doxorubicin (0, 0.0625, 0.125, 0.25, 0.5, 1μg/ml), salinomycin (0, 2.5, 5, 10, 20, 40μM) or PKF 118-310 (0, 0.0625, 0.125, 0.25, 0.5, 1μM). The cells were cultured for 48 h, then 10μl CCK-8 solution was added per well and the plates were incubated for 3 h. Absorbance was then measured at 450 nm using a MRX II microplate reader (Dynex, Chantilly, VA, USA). Relative cell viability was determined as a percentage of untreated control HCC cells.
8
Cell Viability Assessment by CCK-8 Assay
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Cell viability was assessed using the CCK-8 assay (Dojindo Molecular Technologies, Inc.), according to the manufacturer's protocol. Briefly, RCC cells treated with TBOPP or DOCK siRNA for 24 h were seeded (3×104 cells/well) into 96-well plates. Following treatment with cisplatin, 10 µl CCK-8 solution was added to each well and incubated for 2 h at room temperature. The absorbance value of each well at a wavelength of 450 nm was measured using an MRX II microplate reader (Dynex Technologies).
9
Evaluating Cisplatin's Impact on Cell Viability
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A cell counting kit-8 (CCK-8; Dojindo, Kumamoto, Japan) assay was used to determine cell viability. Briefly, BC cells were plated into 96-well plates at a density of 3 × 103 cells/well and incubated overnight at 37°C. Subsequently, cells were treated with a series of concentrations of cisplatin (0, 0.625, 1.25, 2.5, 5, or 10 μM) for 48 h or transfected with HOAXA-AS3 siRNA, miR-455-5p inhibitor, or HOXA-AS3 siRNA plus miR-455-5p inhibitor for 48 h. Then, 10 μl of CCK-8 solution was added to each well and cultured for 3 h at 37°C before the absorbance at 450 nm was measured using an MRX II microplate reader (Dynex Technologies, Chantilly, USA). Relative cell viability was calculated as a percentage of the untreated controls.
10
Evaluating Ni and TNF-α Cytotoxicity in NSCLC
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An equal number of NSCLC cells (5×103 cells/well) was seeded in 96-well plates. Treatments, including TNF-α (20, 40 or 80 ng/ml) and Ni (1 mM as the high dose or 0.5 mM as the low dose) were administered at 37°C 24 h post seeding. Single treatment and combination treatment were used. In the combination treatment, 0.5 nM Ni was used together with 20, 40 or 80 ng/ml TNF-α to study the effects of Ni on TNF-α mediated cell death. Following the specified treatment time (6, 12, 24, 36 or 48 h), cell viability was measured by using a Cell Counting kit-8 (CCK-8; Sigma-Aldrich; Merck KGaA). Briefly, 10 µl CCK-8 solution was added to each well and incubated for 30 min at 37°C. Absorbance was measured at 450 nm using an MRX® II microplate reader (Dynex Technologies, Chantilly, VA, USA). The final cell viability was calculated as: [Treatment optical density (OD) value-blank OD value]/(control OD value-blank OD value). The cell viability was normalized to the control group at 0 h of treatment.
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